Adipocyte MTERF4 regulates non-shivering adaptive thermogenesis and sympathetic-dependent glucose homeostasis

Castillo, Anna; Vila, María; Pedriza, Inés; Pardo, Rosario; Cámara, Yolanda; Martin, Erika; Beiroa, Daniel; Torres‐Torronteras, Javier; Oteo, Marta; Morcillo, Miguel A.; Martí, Ramón; Simó, Rafael; Nogueiras, Rubén; Villena, Josep A.

doi:10.1016/j.bbadis.2019.01.025

Cited by 5 publications

(5 citation statements)

References 57 publications

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“…In vertebrates, the biological function of mTERFs in the regulation of mitochondrial transcription, replication, and translation has been well-documented (Castillo et al, 2019). Although land plants possess more mTERFs than mammals, the functions of mTERFs in plants are rather limited (Kleine and Leister, 2015;Quesada, 2016).…”

Section: Hvmterf Genes May Play Important Roles During Plant Growth Abiotic Stress and Phytohormone Responsesmentioning

confidence: 99%

Identification, Characterization, and Expression Profile Analysis of the mTERF Gene Family and Its Role in the Response to Abiotic Stress in Barley (Hordeum vulgare L.)

Pan

Yuan

et al. 2021

Front. Plant Sci.

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Plant mitochondrial transcription termination factor (mTERF) family regulates organellar gene expression (OGE) and is functionally characterized in diverse species. However, limited data are available about its functions in the agriculturally important cereal barley (Hordeum vulgare L.). In this study, we identified 60 mTERFs in the barley genome (HvmTERFs) through a comprehensive search against the most updated barley reference genome, Morex V2. Then, phylogenetic analysis categorized these genes into nine subfamilies, with approximately half of the HvmTERFs belonging to subfamily IX. Members within the same subfamily generally possessed conserved motif composition and exon-intron structure. Both segmental and tandem duplication contributed to the expansion of HvmTERFs, and the duplicated gene pairs were subjected to strong purifying selection. Expression analysis suggested that many HvmTERFs may play important roles in barley development (e.g., seedlings, leaves, and developing inflorescences) and abiotic stresses (e.g., cold, salt, and metal ion), and HvmTERF21 and HvmTERF23 were significant induced by various abiotic stresses and/or phytohormone treatment. Finally, the nucleotide diversity was decreased by only 4.5% for HvmTERFs during the process of barley domestication. Collectively, this is the first report to characterize HvmTERFs, which will not only provide important insights into further evolutionary studies but also contribute to a better understanding of the potential functions of HvmTERFs and ultimately will be useful in future gene functional studies.

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Section: Hvmterf Genes May Play Important Roles During Plant Growth Abiotic Stress and Phytohormone Responsesmentioning

confidence: 99%

Identification, Characterization, and Expression Profile Analysis of the mTERF Gene Family and Its Role in the Response to Abiotic Stress in Barley (Hordeum vulgare L.)

Pan

Yuan

et al. 2021

Front. Plant Sci.

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“…Gene expression was analyzed as previously described [46], with minor modifications. Briefly, total RNA was first isolated from cells with NZYol (NZYtech) and cDNA was then synthesized from 200 ng of RNA using SuperScript II reverse transcriptase (Invitrogen) and oligo dT primers.…”

Section: Gene Expressionmentioning

confidence: 99%

Enhancing Glycolysis Protects against Ischemia-Reperfusion Injury by Reducing ROS Production

et al. 2020

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After myocardial ischemia-reperfusion, fatty acid oxidation shows fast recovery while glucose oxidation rates remain depressed. A metabolic shift aimed at increasing glucose oxidation has shown to be beneficial in models of myocardial ischemia-reperfusion. However, strategies aimed at increasing glucose consumption in the clinic have provided mixed results and have not yet reached routine clinical practice. A better understanding of the mechanisms underlying the protection afforded by increased glucose oxidation may facilitate the transfer to the clinic. The purpose of this study was to evaluate if the modulation of reactive oxygen species (ROS) was involved in the protection afforded by increased glucose oxidation. Firstly, we characterized an H9C2 cellular model in which the use of glucose or galactose as substrates can modulate glycolysis and oxidative phosphorylation pathways. In this model, there were no differences in morphology, cell number, or ATP and PCr levels. However, galactose-grown cells consumed more oxygen and had an increased Krebs cycle turnover, while cells grown in glucose had increased aerobic glycolysis rate as demonstrated by higher lactate and alanine production. Increased aerobic glycolysis was associated with reduced ROS levels and protected the cells against simulated ischemia-reperfusion injury. Furthermore, ROS scavenger N-acetyl cysteine (NAC) was able to reduce the amount of ROS and to prevent cell death. Lastly, cells grown in galactose showed higher activation of mTOR/Akt signaling pathways. In conclusion, our results provide evidence indicating that metabolic shift towards increased glycolysis reduces mitochondrial ROS production and prevents cell death during ischemia-reperfusion injury.

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“…Unexpectedly in our data, maternal RGYB was able to increase UCP1 expression in BAT of offspring but inefficient to revert the reduction of CI and CIII. Castillo et al 62 showed that the mitochondrial transcription termination factor-4 (MTERF4) absence in BAT leads to reduced OXPHOS mitochondrial protein levels and impaired assembly of OXPHOS CI, III and IV due to deficient translation of mitochondrial DNA-encoded proteins. MTERF4-FAT-KO mice showed low OXPHOS, reduced thermogenesis activity but no alteration in UCP1 protein expression.…”

Section: Discussionmentioning

confidence: 99%

Maternal Roux-en-Y gastric bypass surgery reduces lipid deposition and increases UCP1 expression in the brown adipose tissue of male offspring

Ceglarek

Bertasso

Pietrobon

et al. 2021

Sci Rep

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Maternal obesity induced by cafeteria diet (CAF) predisposes offspring to obesity and metabolic diseases, events that could be avoided by maternal bariatric surgery (BS). Herein we evaluated whether maternal BS is able to modulate brown adipose tissue (BAT) morphology and function in adult male rats born from obese female rats submitted to Roux-en-Y gastric bypass (RYGB). For this, adult male rat offspring were obtained from female rats that consumed standard diet (CTL), or CAF diet, and were submitted to simulated operation or RYGB. Analysis of offspring showed that, at 120 days of life, the maternal CAF diet induced adiposity and decreased the expression of mitochondrial Complex I (CI) and Complex III (CIII) in the BAT, resulting in higher accumulation of lipids than in BAT from offspring of CTL dams. Moreover, maternal RYGB increased UCP1 expression and prevented excessive deposition of lipids in the BAT of adult male offspring rats. However, maternal RYGB failed to reverse the effects of maternal diet on CI and CIII expression. Thus, maternal CAF promotes higher lipid deposition in the BAT of offspring, contributing to elevated adiposity. Maternal RYGB prevented obesity in offspring, probably by increasing the expression of UCP1.

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Adipocyte MTERF4 regulates non-shivering adaptive thermogenesis and sympathetic-dependent glucose homeostasis

Cited by 5 publications

References 57 publications

Identification, Characterization, and Expression Profile Analysis of the mTERF Gene Family and Its Role in the Response to Abiotic Stress in Barley (Hordeum vulgare L.)

Identification, Characterization, and Expression Profile Analysis of the mTERF Gene Family and Its Role in the Response to Abiotic Stress in Barley (Hordeum vulgare L.)

Enhancing Glycolysis Protects against Ischemia-Reperfusion Injury by Reducing ROS Production

Maternal Roux-en-Y gastric bypass surgery reduces lipid deposition and increases UCP1 expression in the brown adipose tissue of male offspring

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