Adipocyte metabolism is improved by TNF receptor-targeting small RNAs identified from dried nuts

Aquilano, Katia; Ceci, Veronica; Gismondi, Angelo; Stefano, Susanna De; Iacovelli, Federico; Faraonio, Raffaella; Marco, Gabriele Di; Poerio, Noemi; Minutolo, Antonella; Minopoli, Giuseppina; Marcone, Antonia; Fraziano, Maurizio; Tortolici, Flavia; Sennato, Simona; Casciardi, Stefano; Potestà, Marina; Bernardini, Roberta; Mattei, Maurizio; Falconi, Mattia; Montesano, Carla; Rufini, Stefano; Canini, Antonella; Barbato, Daniele Lettieri

doi:10.1038/s42003-019-0563-7

Cited by 58 publications

(55 citation statements)

References 63 publications

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“…Zhang 18 and others works 16,20,45 introduced the crosskingdom interaction concept in which miRNAs present in plant extracts, introduced by diet, are able to control gene expression in human cells. Different studies 21,26,46,47 have confirmed this hypothesis: plant sRNAs containing Relative RT-qPCR expression analysis of the most conserved mol-miRNAs contained in the pool of sRNAs extracted from MOES and MOES MVs.…”

Section: Discussionmentioning

confidence: 99%

“…Following the characterization of the miRs content in MOES MVs, a bioinformatic analysis was performed to identify the possible human genes targeted by the miRs present in the mol-sR pool and involved in the apoptosis mechanism 16,20,35 .…”

Section: Computational Prediction Of Genes Targeted By Mol-mirs Recovmentioning

confidence: 99%

“…MiRNAs are short stretches of noncoding RNAs that play an essential role in gene regulation; they regulate protein expression at the post-transcriptional level in animals and plants. More than four hundred conserved miRNAs have been identified from plants, and in silico and in vitro functional validation have highlighted the possible role of plant miRNAs as regulators of protein expression in mammals 11,[14][15][16][17][18][19][20][21] .…”

Section: Introductionmentioning

confidence: 99%

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Effect of microvesicles from Moringa oleifera containing miRNA on proliferation and apoptosis in tumor cell lines

et al. 2020

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Human microvesicles are key mediators of cell-cell communication. Exosomes function as microRNA transporters, playing a crucial role in physiological and pathological processes. Plant microvesicles (MVs) display similar features to mammalian exosomes, and these MVs might enhance plant microRNA delivery in mammals. Considering that plant microRNAs have been newly identified as bioactive constituents in medicinal plants, and that their potential role as regulators in mammals has been underlined, in this study, we characterized MVs purified from Moringa oleifera seeds aqueous extract (MOES MVs) and used flow cytometry methods to quantify the ability to deliver their content to host cells. The microRNAs present in MOES MVs were characterized, and through a bioinformatic analysis, specific human apoptosis-related target genes of plant miRNAs were identified. In tumor cell lines, MOES MVs treatment reduced viability, increased apoptosis levels associated with a decrease in B-cell lymphoma 2 protein expression and reduced mitochondrial membrane potential. Interestingly, the effects observed with MOES MVs treatment were comparable to those observed with MOES treatment and transfection with the pool of small RNAs isolated from MOES, used as a control. These results highlight the role of microRNAs transported by MOES MVs as natural bioactive plant compounds that counteract tumorigenesis.

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Section: Discussionmentioning

confidence: 99%

Section: Computational Prediction Of Genes Targeted By Mol-mirs Recovmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of microvesicles from Moringa oleifera containing miRNA on proliferation and apoptosis in tumor cell lines

et al. 2020

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“…Dried nuts also contain microRNAs (miR156c and miR159a) that exert an anti-inflammatory action by targeting TNF-α receptor in mammalian adipose tissue. In mice, these molecules were associated with a reduction of inflammatory cytokines in adipocytes and visceral adipose depots upon different pro-inflammatory conditions such as hypoxia, cellular hypertrophy, diet-induced obesity, in association with the downregulation of the TNF-α inflammatory signaling pathway [47]. Table 1 presents the amounts of energy, fats, dietary fiber, selenium, and phenolic compounds in 1 portion (30 g) of the main nuts.…”

Section: Nut Composition and Its Implication On Body Weight And Cardimentioning

confidence: 99%

Adipokines and Adipose Tissue-Related Metabolites, Nuts and Cardiovascular Disease

Weschenfelder¹,

Quadros²,

Santos³

et al. 2020

Metabolites

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Adipose tissue is a complex structure responsible for fat storage and releasing polypeptides (adipokines) and metabolites, with systemic actions including body weight balance, appetite regulation, glucose homeostasis, and blood pressure control. Signals sent from different tissues are generated and integrated in adipose tissue; thus, there is a close connection between this endocrine organ and different organs and systems such as the gut and the cardiovascular system. It is known that functional foods, especially different nuts, may be related to a net of molecular mechanisms contributing to cardiometabolic health. Despite being energy-dense foods, nut consumption has been associated with no weight gain, weight loss, and lower risk of becoming overweight or obese. Several studies have reported beneficial effects after nut consumption on glucose control, appetite suppression, metabolites related to adipose tissue and gut microbiota, and on adipokines due to their fatty acid profile, vegetable proteins, l-arginine, dietary fibers, vitamins, minerals, and phytosterols. The aim of this review is to briefly describe possible mechanisms implicated in weight homeostasis related to different nuts, as well as studies that have evaluated the effects of nut consumption on adipokines and metabolites related to adipose tissue and gut microbiota in animal models, healthy individuals, and primary and secondary cardiovascular prevention.

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“…Indeed, it was demonstrated that exosome-like nanoparticles (EPDELNs) of edible plant could determine EPDELN-mediated interspecies communication by eliciting anti-inflammatory cytokines genes, anti-oxidation, and activation of Wnt signaling, which are critical for preserving intestinal homeostasis (Mu et al, 2014). In exosome-like nut nanovesicles, plant miR159a and miR156c reduce the level of tumor necrosis factor (TNF) receptor superfamily member 1a (Tnfrsf1a) transcript leading to a decrease in TNF-α signaling pathway and inflammatory markers (Aquilano et al, 2019). Moreover, it was recently reported that MIR167e-5p represses intestinal cell proliferation by targeting β-catenin (Li et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Plant miRNAs Reduce Cancer Cell Proliferation by Targeting MALAT1 and NEAT1: A Beneficial Cross-Kingdom Interaction

Marzano

Caratozzolo

Consiglio³

et al. 2020

Front. Genet.

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MicroRNAs (miRNAs) are ubiquitous regulators of gene expression, evolutionarily conserved in plants and mammals. In recent years, although a growing number of papers debate the role of plant miRNAs on human gene expression, the molecular mechanisms through which this effect is achieved are still not completely elucidated. Some evidence suggest that this interaction might be sequence specific, and in this work, we investigated this possibility by transcriptomic and bioinformatics approaches. Plant and human miRNA sequences from primary databases were collected and compared for their similarities (global or local alignments). Out of 2,588 human miRNAs, 1,606 showed a perfect match of their seed sequence with the 5 end of 3,172 plant miRNAs. Further selections were applied based on the role of the human target genes or of the miRNA in cell cycle regulation (as an oncogene, tumor suppressor, or a biomarker for prognosis, or diagnosis in cancer). Based on these criteria, 20 human miRNAs were selected as potential functional analogous of 7 plant miRNAs, which were in turn transfected in different cell lines to evaluate their effect on cell proliferation. A significant decrease was observed in colorectal carcinoma HCT116 cell line. RNA-Seq demonstrated that 446 genes were differentially expressed 72 h after transfection. Noteworthy, we demonstrated that the plant mtr-miR-5754 and gma-miR4995 directly target the tumor-associated long non-coding RNA metastasisassociated lung adenocarcinoma transcript 1 (MALAT1) and nuclear paraspeckle assembly transcript 1 (NEAT1) in a sequence-specific manner. In conclusion, according to other recent discoveries, our study strengthens and expands the hypothesis that plant miRNAs can have a regulatory effect in mammals by targeting both protein-coding and non-coding RNA, thus suggesting new biotechnological applications.

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Adipocyte metabolism is improved by TNF receptor-targeting small RNAs identified from dried nuts

Cited by 58 publications

References 63 publications

Effect of microvesicles from Moringa oleifera containing miRNA on proliferation and apoptosis in tumor cell lines

Effect of microvesicles from Moringa oleifera containing miRNA on proliferation and apoptosis in tumor cell lines

Adipokines and Adipose Tissue-Related Metabolites, Nuts and Cardiovascular Disease

Plant miRNAs Reduce Cancer Cell Proliferation by Targeting MALAT1 and NEAT1: A Beneficial Cross-Kingdom Interaction

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