AdipoAtlas: A reference lipidome for human white adipose tissue

Lange, M.; Angelidou, Georgia; Ni, Zhixu; Criscuolo, Angela; Schiller, Jürgen; Blüher, Matthias; Fedorova, Maria

doi:10.1016/j.xcrm.2021.100407

Cited by 72 publications

(80 citation statements)

References 71 publications

(110 reference statements)

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“…The compound annotation has been evaluated using the Kendrick mass defect (KMD) to the CH 2 base [53]. The KMD value of each compound has been plotted versus the specific retention time (Figure S2).…”

Section: Methods Validationmentioning

confidence: 99%

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Grape Lipidomics: An Extensive Profiling thorough UHPLC-MS/MS Method

et al. 2021

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Lipids play many essential roles in living organisms, which accounts for the great diversity of these amphiphilic molecules within the individual lipid classes, while their composition depends on intrinsic and extrinsic factors. Recent developments in mass spectrometric methods have significantly contributed to the widespread application of the liquid chromatography-mass spectrometry (LC–MS) approach to the analysis of plant lipids. However, only a few investigators have studied the extensive composition of grape lipids. The present work describes the development of an ultrahigh performance liquid chromatography tandem mass spectrometry (UHPLC–MS/MS) method that includes 8098 MRM; the method has been validated using a reference sample of grapes at maturity with a successful analysis and semi-quantification of 412 compounds. The aforementioned method was subsequently applied also to the analysis of the lipid profile variation during the Ribolla Gialla cv. grape maturation process. The partial least squares (PLS) regression model fitted to our experimental data showed that a higher proportion of certain glycerophospholipids (i.e., glycerophosphoethanolamines, PE and glycerophosphoglycerols, PG) and of some hydrolysates from those groups (i.e., lyso-glycerophosphocholines, LPC and lyso-glycerophosphoethanolamines, LPE) can be positively associated with the increasing °Brix rate, while a negative association was found for ceramides (CER) and galactolipids digalactosyldiacylglycerols (DGDG). The validated method has proven to be robust and informative for profiling grape lipids, with the possibility of application to other studies and matrices.

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Section: Methods Validationmentioning

confidence: 99%

“…To ensure a confident lipid identification, we manually curated lipid annotations by plotting the retention time of a given lipid species against its KMD value to the CH 2 base [53]. The IS mix was used to assess the recovery and to build the calibration curves determining the range of linearity and limit of detection (LOD).…”

Section: Methods Validationmentioning

confidence: 99%

Grape Lipidomics: An Extensive Profiling thorough UHPLC-MS/MS Method

et al. 2021

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“…These differences likely reflect the metabolic activity and function of each tissue, as phospholipids regulate UCP1 within mitochondrial inner membranes of thermogenic BAT cells, whilst TG species are typically stored in WAT ( Leiria and Tseng, 2020 ). Despite the lower levels of ether lipids in SAT compared to BAT, lipidomic analysis of human WAT reported that plasmalogens are still highly abundant relative to other lipid species ( Lange et al, 2021 ). Findings showed that PE plasmalogens were the most abundant species containing PUFA as well as the fourth most abundant lipid class overall.…”

Section: In Vitro and In Vivo Studiesmentioning

confidence: 99%

“…Findings showed that PE plasmalogens were the most abundant species containing PUFA as well as the fourth most abundant lipid class overall. Interestingly, PE plasmalogen levels were around 3 times higher than PE-diacyl species ( Lange et al, 2021 ). Interestingly, another study that conducted lipidomic analysis on primary brown, white and beige adipocytes in vitro revealed contrary results ( Schweizer et al, 2019 ).…”

Section: In Vitro and In Vivo Studiesmentioning

confidence: 99%

“…Whilst our understanding of the adipose lipidome remains limited, particularly in relation to ether lipids, research has begun to explore the apparent remodelling of adipose tissue in the setting of obesity. Multiple studies have now comprehensively analysed the lipidomic signatures of adipose tissue from obese and lean individuals ( Pietiläinen et al, 2011 ; Lange et al, 2021 ). One study conducted by Pietiläinen et al (2011) revealed unique changes in the composition of ether lipids in twin pairs discordant for obesity ( Pietiläinen et al, 2011 ).…”

Section: In Vitro and In Vivo Studiesmentioning

confidence: 99%

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Ether Lipids in Obesity: From Cells to Population Studies

et al. 2022

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Ether lipids are a unique class of glycero- and glycerophospho-lipid that carry an ether or vinyl ether linked fatty alcohol at the sn-1 position of the glycerol backbone. These specialised lipids are important endogenous anti-oxidants with additional roles in regulating membrane fluidity and dynamics, intracellular signalling, immunomodulation and cholesterol metabolism. Lipidomic profiling of human population cohorts has identified new associations between reduced circulatory plasmalogen levels, an abundant and biologically active sub-class of ether lipids, with obesity and body-mass index. These findings align with the growing body of work exploring novel roles for ether lipids within adipose tissue. In this regard, ether lipids have now been linked to facilitating lipid droplet formation, regulating thermogenesis and mediating beiging of white adipose tissue in early life. This review will assess recent findings in both population studies and studies using cell and animal models to delineate the functional and protective roles of ether lipids in the setting of obesity. We will also discuss the therapeutic potential of ether lipid supplementation to attenuate diet-induced obesity.

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The lipid droplet lipidome

Wölk,

Fedorova

2024

FEBS Letters

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Lipid droplets (LDs) are intracellular organelles with a hydrophobic core formed by neutral lipids surrounded by a phospholipid monolayer harboring a variety of regulatory and enzymatically active proteins. Over the last few decades, our understanding of LD biology has evolved significantly. Nowadays, LDs are appreciated not just as passive energy storage units, but rather as active players in the regulation of lipid metabolism and quality control machineries. To fulfill their functions in controlling cellular metabolic states, LDs need to be highly dynamic and responsive organelles. A large body of evidence supports a dynamic nature of the LD proteome and its contact sites with other organelles. However, much less is known about the lipidome of LDs. Numerous examples clearly indicate the intrinsic link between LD lipids and proteins, calling for a deeper characterization of the LD lipidome in various physiological and pathological settings. Here, we reviewed the current state of knowledge in the field of the LD lipidome, providing a brief overview of the lipid classes and their molecular species present within the neutral core and phospholipid monolayer.

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AdipoAtlas: A reference lipidome for human white adipose tissue

Cited by 72 publications

References 71 publications

Grape Lipidomics: An Extensive Profiling thorough UHPLC-MS/MS Method

Grape Lipidomics: An Extensive Profiling thorough UHPLC-MS/MS Method

Ether Lipids in Obesity: From Cells to Population Studies

The lipid droplet lipidome

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