Adhesiveness of opportunistic Staphylococcus aureus to materials used in dental office: In vitro study

Merghni, Abderrahmen; Békir, Karima; Kadmi, Yassine; Dallel, Ines; Janel, Sébastien; Bovio, Simone; Barois, Nicolas; Lafont, Frank; Mastouri, Maha

doi:10.1016/j.micpath.2016.12.014

Cited by 11 publications

(11 citation statements)

References 39 publications

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“…The microbiological finding of Staphylococcus spp. on PTFE surfaces in our study agrees with what has been shown in a series of investigations by Merghni and coworkers (Merghni et al, 2016; Merghni et al, 2017; Merghni, Ben Nejma, Hentati, Mahjoub, & Mastouri, 2014). The authors evaluated the adhesive ability of oral staphylococcal strains isolated from the oral cavity and orthodontic appliances or in vitro from different abiotic surfaces commonly encountered in dental practices.…”

Section: Discussionsupporting

confidence: 93%

Early plaque formation on PTFE membranes with expanded or dense surface structures applied in the oral cavity of human volunteers

Turri

Čirgić

Shah

et al. 2020

Clinical & Exp Dental Res

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Objectives This clinical randomized study aimed to evaluate the early plaque formation on nonresorbable polytetrafluoroethylene (PTFE) membranes having either a dense (d‐PTFE) or an expanded (e‐PTFE) microstructure and exposed to the oral cavity. Material and Methods Twelve individuals were enrolled in this study. In a split‐mouth design, five test membranes (e‐PTFE) with a dual‐layer configuration and five control membranes (d‐PTFE) were bonded on the buccal surfaces of posterior teeth of each subject. All study subjects refrained from toothbrushing during the study period. Specimens were detached from the teeth at 4 and 24 hr and subjected to viability counting, confocal microscopy, and scanning electron microscopy. Plaque samples were harvested from neighboring teeth at baseline, 4, and 24 hr, as control. Wilcoxon signed rank test was applied. Results No bond failure of the membranes was reported. Between the early and late time points, viable bacterial counts increased on all membranes, with no difference between the test and control. The number of Staphylococcus spp. decreased on the tooth surfaces and increased on both membranes overtime, with a significant difference compared to teeth. The total biomass and average biofilm thickness of live and dead cells were significantly greater at the d‐PTFE barriers after 4 hr. Conclusion This study demonstrated that the e‐PTFE membrane was associated with a lesser degree of biofilm accumulation during the initial exposure compared to the d‐PTFE membrane. The present experimental setup provides a valuable toolbox to study the in vivo behavior of different membranes used in guided bone regeneration (GBR).

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Section: Discussionsupporting

confidence: 93%

Early plaque formation on PTFE membranes with expanded or dense surface structures applied in the oral cavity of human volunteers

Turri

Čirgić

Shah

et al. 2020

Clinical & Exp Dental Res

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“…EPS adherent to the medically surface (skin, implants, wearing) are accounting for most of microbial infections in the internal fixation devices [16]. Bioinert surfaces attract the biofilm formation [18–20]. The ligand of bacterial fimbriae can be bound to electrovalent bond or hydrophobic bond on the surface of the material.…”

Section: Mechanism Of the Iris—biofilmmentioning

confidence: 99%

Coatings as the useful drug delivery system for the prevention of implant-related infections

Pan

Zhou

2018

J Orthop Surg Res

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Implant-related infections (IRIs) which led to a large amount of medical expenditure were caused by bacteria and fungi that involve the implants in the operation or in ward. Traditional treatments of IRIs were comprised of repeated radical debridement, replacement of internal fixators, and intravenous antibiotics. It needed a long time and numbers of surgeries to cure, which meant a catastrophe to patients. So how to prevent it was more important than to cure it. As an excellent local release system, coating is a good idea by its local drug infusion and barrier effect on resisting biofilms which were the main cause of IRIs. So in this review, materials used for coatings and evidences of prevention were elaborated.

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“…It is possible that additional hydrogen and ionic bonds can be established on a conditioning film formed on the substratum surface [59]. Whether these are formed or are stronger than the binding of the conditioning film to the surface, depends on the bacterial species, the binding partners involved and the subsurface [4,29,38,60]. For instance, we observe higher rupture length for BP coatings on the surface but no increase in adhesion force compared to the uncoated state.…”

Section: Sample Characterization Xps Reveals Thatmentioning

confidence: 77%

“…The contact angle is a typical surface property measured in combination with adhesion. It has been shown that the hydrophobicity greatly influences the adhesion of bacteria to a surface in both ways, but in general bacteria adhere better to hydrophobic surfaces [29,32,56,57]. The contact angle is amongst other properties influenced by surface roughness which often varies between surfaces.…”

Section: Sample Characterization Xps Reveals Thatmentioning

confidence: 99%

“…Single-cell force spectroscopy (SCFS) with an atomic force microscope has proven to be an ideal method to determine the adhesion parameters of bacterial cell wall macromolecules forming interactions with the surface [24][25][26]. For the purpose of this study, we have chosen the opportunistic pathogen Staphylococcus aureus, as it forms clinically relevant biofilms [27], and is a common cause of implant failures and inflammation in the oral cavity [28][29][30] and beyond [10].…”

Section: Introductionmentioning

confidence: 99%

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Hydroxyapatite pellets as versatile model surfaces for systematic studies on enamel

Mischo

Faidt

McMillan

et al. 2021

Preprint

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Research into materials for medical application draws inspiration from naturally occurring or synthesized surfaces, just like many other research directions. For medical application of materials, particular attention has to be paid to biocompatibility, osseointegration and bacterial adhesion behavior. To understand their properties and behavior, experimental studies with natural materials such as teeth are strongly required. The results, however, may be highly case-dependent because natural surfaces have the disadvantage of being subject to wide variations, for instance in their chemical composition, structure, morphology, roughness, and porosity. A synthetic surface which mimics enamel in its performance with respect to bacterial adhesion and biocompatibility would, therefore, facilitate systematic studies much better. In this study, we discuss the possibility of using hydroxyapatite (HAp) pellets to simulate the surfaces of teeth and show the possibility and limitations of using a model surface. We performed single-cell force spectroscopy with single Staphylococcus aureus cells to measure adhesion-related parameters such as adhesion force and rupture length of adhesins binding to HAp and enamel. We also examine the influence of blood plasma and saliva on the adhesion properties of S. aureus. The results of these measurements are matched to water wettability, elemental composition of the samples and the change in the macromolecules adsorbed over time. We found that the adhesion properties of S. aureus were similar on both samples under all conditions: Significant decreases in adhesion strength were found equally in the presence of saliva or blood plasma on both surfaces. We therefore conclude that HAp pellets are a good alternative for natural dental material. This is especially true when slight variations in the physicochemical properties of the natural materials may affect the experimental series.

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Adhesiveness of opportunistic Staphylococcus aureus to materials used in dental office: In vitro study

Cited by 11 publications

References 39 publications

Early plaque formation on PTFE membranes with expanded or dense surface structures applied in the oral cavity of human volunteers

Early plaque formation on PTFE membranes with expanded or dense surface structures applied in the oral cavity of human volunteers

Coatings as the useful drug delivery system for the prevention of implant-related infections

Hydroxyapatite pellets as versatile model surfaces for systematic studies on enamel

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