Adhesive strength of marine mussel extracts on porcine skin

Ninan, Lal

doi:10.1016/s0142-9612(03)00257-6

Cited by 148 publications

(92 citation statements)

References 49 publications

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“…These problems have made difficult to accept for wide use. In spite of the possible complications, many authors have considered ECA a safe bio-adhesive for use in vivo due to the qualities it shares with the other CAs such as its resistance to exuded actions, the ability to support up to a traction of 500g, fast polymerization, provisory toxicity and related effects, bactericide and hemostatic effects when applied to the wound 2,6,12 . Furthermore, many studies have suggested a more widespread use of ECA over other CAs due to its low cost, fast degradation and ample disposability 4,8,14,15 .…”

Section: Discussionmentioning

confidence: 99%

“…The ideal adhesive must provide adequate tensile strength against biological fluids and keep tissues joined until the proper cicatrisation occurs, but it should not alter immune system function in the wound. Furthermore, it should be biodegradable, nontoxic, readily available and quick drying 2,3 . Since the end of the 1950's, synthetic adhesives have been used in medical applications 4 .…”

Section: Introductionmentioning

confidence: 99%

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Comparative study of suture and cyanoacrylates in skin closure of rats

Souza¹,

Oliveira²,

Soares³

et al. 2007

Acta Cir. Bras.

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Purpose:To compare the biocompatibility of ethyl-cyanoacrylate (ECA) and octylcyanoacrylate (OCA) wound closures to sutures in rat skin. Methods: Twenty-four male Wistar rats were subjected to three incisions which were closed using ECA, OCA or sutures . Rats were divided into four groups which received biopsies on the 3 rd , 7 th , 14 th or 21 st postoperative days. Necrosis, inflammation, dermatitis, infection, dehiscence, cicatricial enlargement and costs were examined; the histopathology evaluated was epithelialization, deep openings, foreign substance reaction, residues of synthesis material, fibrosis, inflammation, dehiscence and necrosis. Results: The tissue adhesives presented the largest dehiscence levels, and ECA the lowest cost while the other measures were similar. Regarding histopathology, deep openings were more common with OCA and granulomas were most frequently obtained with ECA. The two tissue adhesives produces less inflammation than the inicial suture from post-operative day 7, while ECA and OCA cause similar inflammatory reactions. ECA did not differ significantly from OCA and sutures on other measures. Conclusion: ECA was well tolerated in this study and did not induce necrosis, allergic reactions or infections, presenting several advantages in relation to OCA and sutures, including lower costs and fewer complications. Key words: Tissue adhesives. Cyanoacrylates. Materials Testing. Rats. RESUMOObjetivo: Investigar a biocompatibilidade do etil-cianoacrilato (ECA) em fechamento de pele em ratos comparativamente ao octil-cianoacrilato (OCA) e à sutura. Métodos: Rattus norvegicus albinus (n=24) foram submetidos a três incisões, cada uma fechada por um dos métodos de síntese estudados. Quatro grupos (n=6) foram feitos, conforme o dia pós-operatório (DPO) em que foram eutanasiados: 3DPO, 7DPO,14DPO e 21DPO. Necrose, edema, eritema, dermatite, infecção, deiscência, alargamento cicatricial e custos foram os itens examinados; histopatologicamente avaliou-se epitelização, abertura profunda, reação de corpo estranho, resíduos de material de síntese, fibrose, reação inflamatória, deiscência e necrose. Resultados: Os adesivos apresentaram os maiores níveis de deiscências, e o ECA o custo mais baixo; nos demais itens, não houve diferenças. Histopatologia: A abertura profunda foi mais comum com o OCA; ECA foi o material mais encontrado em granulomas; os adesivos foram mais brandos que a sutura a partir do 7º DPO no quesito inflamação, enquanto que o ECA causou uma reação similar ao OCA; nos outros itens, ECA não apresentou diferença importante em relação ao OCA e à sutura. Conclusão: O ECA foi bem tolerado neste grupo de estudo, sem induzir necrose, reações alérgicas e infecção, apresentando diversas vantagens de uso em relação ao OCA e à sutura. Descritores: Adesivos Teciduais. Cianoacrilatos. Teste de Materiais. Ratos. -ORIGINAL ARTICLE Souza SC et al 310

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparative study of suture and cyanoacrylates in skin closure of rats

Souza¹,

Oliveira²,

Soares³

et al. 2007

Acta Cir. Bras.

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“…These investigators used distinctly different curing conditions and joint configurations, which makes direct comparison cumbersome. In a previous paper Ninan et al [41] demonstrated that extracts of mussel adhesive protein formed strong bonds with porcine skin. However, the time required to achieve a strong bond was between 12 and 16 h when the adhesive was incubated in a humid environment at 37°C.…”

Section: Introductionmentioning

confidence: 99%

Adhesive strength and curing rate of marine mussel protein extracts on porcine small intestinal submucosa

et al. 2007

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An adhesive protein extracted from marine mussel (Mytilus edulis) was used to bond strips of connective tissue for the purpose of evaluating the use of curing agents to improve adhesive curing. Specifically, mussel adhesive protein solution (MAPS, 0.5 mM dihydroxyphenylalanine) was applied, with or without the curing agents, to the ends of two overlapping strips of porcine small intestinal submucosa. The bond strength of this lap joint was determined after curing for 1 h at room temperature (25°C). The strength of joints formed using only MAPS or with only the ethyl, butyl or octyl cyanoacrylate adhesives were determined. Although joints bonded using ethyl cyanoacrylate were strongest, those using MAPS were stronger than those using butyl and octyl cyanoacrylates. The addition of 25 mM solutions of the transition metal ions V 5+ , Fe 3+ and Cr 6+ , which are all oxidants, increased the bond strength of the MAPS joints. The V 5+ gave the strongest bonds and the Fe 3+ the second strongest. In subsequent tests with V 5+ and Fe 3+ solutions, the bond strength increased with V 5+ concentration, but it did not increase with Fe 3+ concentration. Addition of 250 mM V 5+ gave a very strong bond.

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“…Purified mussel adhesive proteins have been investigated on pig skin tissue and bonding strength was tested (Ninan et al 2003). Despite some limitations for possible clinical use, the results of this study suggested the potential use of Mefp as an adhesive for soft tissues.…”

Section: (I) Adhesion In Underwater Applicationsmentioning

confidence: 99%

Mimicking biopolymers on a molecular scale: nano(bio)technology based on engineered proteins

Grunwald

Rischka

Kast

et al. 2009

Phil. Trans. R. Soc. A.

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Proteins are ubiquitous biopolymers that adopt distinct three-dimensional structures and fulfil a multitude of elementary functions in organisms. Recent systematic studies in molecular biology and biotechnology have improved the understanding of basic functional and architectural principles of proteins, making them attractive candidates as concept generators for technological development in material science, particularly in biomedicine and nano(bio)technology. This paper highlights the potential of molecular biomimetics in mimicking high-performance proteins and provides concepts for applications in four case studies, i.e. spider silk, antifreeze proteins, blue mussel adhesive proteins and viral ion channels.

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Adhesive strength of marine mussel extracts on porcine skin

Cited by 148 publications

References 49 publications

Comparative study of suture and cyanoacrylates in skin closure of rats

Comparative study of suture and cyanoacrylates in skin closure of rats

Adhesive strength and curing rate of marine mussel protein extracts on porcine small intestinal submucosa

Mimicking biopolymers on a molecular scale: nano(bio)technology based on engineered proteins

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