Adhesive protein expression on human endothelial cells after in vitro contact with woven Dacron

Granchi, Donatella; Cenni, E.; Verri, E.; Ciapetti, G.; Gori, Alessandra; Gamberini, Simonetta; Leo, Alessandro Di; Pizzoferrato, Alberto

doi:10.1016/s0142-9612(97)00161-0

Cited by 32 publications

(19 citation statements)

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“…[9][10][11] In contrast, the MFI of ICAM-1 expression increases during proliferation, indicating a gradual and specific activation of ECs. The fact that only the ICAM-1 expression is affected could be an indication of the way cell activation is triggered.…”

Section: Discussionmentioning

confidence: 95%

ICAM‐1 and VCAM‐1 expression by endothelial cells grown on fibronectin‐coated TCPS and PS

Zijpp

Poot

Feijén

2003

J Biomedical Materials Res

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Small-diameter vascular grafts rapidly fail as a result of blood coagulation and platelet deposition. Endothelial cells lining the inner side of blood vessels can provide the graft lumen with an antithrombogenic surface. One of the remaining problems is cell detachment after restoration of blood flow, because of infiltration of leukocytes that respond to an inflammatory-like activation of the endothelial cells. This endothelial activation is possibly caused by the surface characteristics of the underlying polymer. To get more insight into the effects of the polymer surface on endothelial cell activation, we seeded human umbilical vein endothelial cells (HUVECs) in various densities and subsequently grew them on tissue culture polystyrene (TCPS; hydrophilic) and polystyrene (PS; hydrophobic) surfaces. To improve cell adhesion, surfaces were coated with purified fibronectin prior to cell seeding. During proliferation, the expressions of the leukocyte adhesion molecules ICAM-1 and VCAM-1 were determined. Results indicate that ICAM-1 expression is not influenced by the character of the polymer surface, and that VCAM-1 expression is slightly higher on the TCPS surface. Expressions of both adhesion molecules are influenced by the seeding density and time of proliferation. At low seeding densities (< or = 10,000 cells/cm(2)), a relatively low percentage of nonexogenously activated cells expressed ICAM-1 during the first 3 days of proliferation compared to higher seeding densities. Although less pronounced, this was also observed for the percentage of cells expressing VCAM-1. During proliferation, the amount of ICAM-1 per endothelial cell increased, whereas the expression of VCAM-1 remained low. The absence of large differences in leukocyte adhesion molecule expression by endothelial cells grown on TCPS or PS is possibly caused by coating of the surfaces with fibronectin. It is known that surface hydrophilicity influences protein adsorption. Although this had no or little effect on leukocyte adhesion molecule expression, endothelial cell growth was affected, because proliferation was slower on the hydrophobic PS.

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Section: Discussionmentioning

confidence: 95%

ICAM‐1 and VCAM‐1 expression by endothelial cells grown on fibronectin‐coated TCPS and PS

Zijpp

Poot

Feijén

2003

J Biomedical Materials Res

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“…A 2-h preactivation of neutrophils may not be long enough to induce activation of EC agonists; however, it is more likely the time of preactivation was too long given that previous studies have shown upregulation of CD11b, L-selectin shedding, and oxidative burst in neutrophils exposed to different biomaterials and other agonists after 30 min. 11,18 Another consideration that should be made in assessing the role of biomaterial-treated neutrophils on EC proinflammatory phenotype is the time of coculture with EC as Mesri and Altieri 21 used a 10-h incubation time for coculture of neutrophils with EC.…”

Section: Discussionmentioning

confidence: 99%

Proinflammatory phenotype of endothelial cells after coculture with biomaterial‐treated blood cells

Lester¹,

Babensee²

2003

J Biomedical Materials Res

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An understanding of the endothelial cell/blood/ biomaterial interactions is central to advancing the success of cardiovascular devices that continue to fail because of the lack of nonthrombogenic biomaterials. A simplified endothelial cell/blood cell/biomaterial static model was used to assess these interactions. Human whole blood or isolated blood cells (mononuclear cells, neutrophils, platelets) were pretreated with biomaterial beads with different surface chemistries: polystyrene (PS), PS beads grafted with 3-kDa polyethylene glycol (PEG) with either hydroxyl (PS-PEG-OH) or amine (PS-PEG-NH 2 ) terminal groups at bead concentrations of 5.4 or 54 × 10 4 beads/mL. Leukocyte and platelet activation and microparticle formation was assessed using flow cytometry. Biomaterial-activated whole blood or isolated cells or mononuclear cell fractions were applied to human umbilical cord endothelial cells (HUVEC) for static coculture, and the resultant proinflammatory HUVEC phenotype was characterized. ICAM-1 and E-selectin expression on HUVEC was increased after 4-h static coculture with biomaterial-treated human whole blood or mononuclear cells but not neutrophils or platelets. VCAM-1 expression on HUVEC was similarly increased after 24-h static coculture but not after 4 h of coculture. Increased concentrations of cytokines, IL-6, IL-8, and MCP-1, were detected in the supernatant of cocultures of HUVEC with biomaterial-treated whole blood or mononuclear cells but not neutrophils or platelets, compared with the media control. After 24 h, cytokine release was significantly increased for both IL-8 and MCP-1 but not IL-6 above concentrations after 4 h of coculture. Neither the cell adhesion molecule (CAM) expression nor cytokine release induced by coculture with biomaterialtreated whole blood or isolated cells was dependent on either material surface chemistry or material surface area. The changes in HUVEC CAM expression and cytokine release induced by biomaterial-treated mononuclear cells can be attributed predominantly to adherent cells on beads and nonadherent bulk cells with moderate regulation by the soluble supernatant; however, mononuclear cell-derived microparticles induced no significant changes in CAM expression or cytokine release after static coculture with HUVEC.

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“…CD31 belongs to the superfamily of the immunoglobulin and occurs to regulate EC-EC adhesion and EC-leukocyte adhesion. It is usually believed that an increased expression of CD31 is in favor of endothelialization [29], while a decreased expression of CD31 by EC is a possible implication of cell damage [30]. CD106 and CD54 belong to the immunoglobulin superfamily as well, and mainly regulate EC-leukocyte adhesion [29,[31][32][33].…”

Section: Ec Phenotype Analysismentioning

confidence: 99%

“…It is usually believed that an increased expression of CD31 is in favor of endothelialization [29], while a decreased expression of CD31 by EC is a possible implication of cell damage [30]. CD106 and CD54 belong to the immunoglobulin superfamily as well, and mainly regulate EC-leukocyte adhesion [29,[31][32][33]. The expression of all three surface makers by the ECs cultured on the gelatin-modified PET NFM indicates the preservation of EC's characteristic phenotype.…”

Section: Ec Phenotype Analysismentioning

confidence: 99%

Surface engineering of electrospun polyethylene terephthalate (PET) nanofibers towards development of a new material for blood vessel engineering

et al. 2005

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Adhesive protein expression on human endothelial cells after in vitro contact with woven Dacron

Cited by 32 publications

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ICAM‐1 and VCAM‐1 expression by endothelial cells grown on fibronectin‐coated TCPS and PS

ICAM‐1 and VCAM‐1 expression by endothelial cells grown on fibronectin‐coated TCPS and PS

Proinflammatory phenotype of endothelial cells after coculture with biomaterial‐treated blood cells

Surface engineering of electrospun polyethylene terephthalate (PET) nanofibers towards development of a new material for blood vessel engineering

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