Adhesive properties of five mesophilic, cellulolytic Clostridia isolated from the same biotope

Gelhaye, Éric; Benoit, Lydie; Petitdemange, H.; Gay, Robert

doi:10.1016/0378-1097(93)90001-i

Cited by 2 publications

(2 citation statements)

References 7 publications

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“…The effect of shredding and mechanical or freeze milling would be to give respective increases in the surface area of the paper available for bacterial adhesion. Although clostridia from MSW adhere less well to newspaper than to filter paper (Gelhaye et al 1993), the expected consequence of increasing the surface area of any form of cellulose would be to increase its susceptibility to microbial degradation (Weimer et al 1991). However, there was no significant effect of any of the pretreatments on substrate solubilization.…”

Section: Discussionmentioning

confidence: 96%

Newspaper as a substrate for cellulolytic landfill bacteria

Cummings¹,

Stewart²

1994

Journal of Applied Bacteriology

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Five cellulolytic bacterial isolates (Clostridium and Eubacterium spp.) from a methane‐producing landfill were examined to determine their ability to utilize newspaper as a substrate for growth. Solubilization was poor with even the most actively cellulolytic bacteria. The major factor causing the low activity seemed to be that as much as 24% of the newspaper was composed of the high molecular weight polymer lignin, which exerts a protective effect on the attack of otherwise susceptible polymers. The presence of ink on heavily printed paper also reduced the rate of cellulose solubilization. Although the ink did not appear directly toxic to the bacteria it masked the surface of the paper, covering the cellulose fibres and preventing bacterial adhesion to the substrate. The action of the cellulolytic isolates was also strongly inhibited below the optimum growth temperature of 37°C.

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Section: Discussionmentioning

confidence: 96%

Newspaper as a substrate for cellulolytic landfill bacteria

Cummings¹,

Stewart²

1994

Journal of Applied Bacteriology

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“…In the early stages of batch cultures, C. cellulolyticum is essentially found in close contact with cellulose and the release of bacterial cells at the end of growth is correlated with the exhaustion of accessible cellulose [108,109]. Gelhaye et al showed that adhesion to cellulose was a two step process [110,111], where reversible and site specific cell–cellulose interaction occurs first, followed by cell–cell interaction due to aggregation of cells on the surface of the insoluble substrate. Cells in the upper layer of the biofilm are not in direct contact with the insoluble substrate.…”

Section: The Physiology Of C Cellulolyticum On Cellulosementioning

confidence: 99%

Clostridium cellulolyticum: model organism of mesophilic cellulolytic clostridia

Desvaux¹

2005

FEMS Microbiol Rev

182

102

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Clostridium cellulolyticum ATCC 35319 is a non-ruminal mesophilic cellulolytic bacterium originally isolated from decayed grass. As with most truly cellulolytic clostridia, C. cellulolyticum possesses an extracellular multi-enzymatic complex, the cellulosome. The catalytic components of the cellulosome release soluble cello-oligosaccharides from cellulose providing the primary carbon substrates to support bacterial growth. As most cellulolytic bacteria, C. cellulolyticum was initially characterised by limited carbon consumption and subsequent limited growth in comparison to other saccharolytic clostridia. The first metabolic studies performed in batch cultures suggested nutrient(s) limitation and/or by-product(s) inhibition as the reasons for this limited growth. In most recent investigations using chemostat cultures, metabolic flux analysis suggests a self-intoxication of bacterial metabolism resulting from an inefficiently regulated carbon flow. The investigation of C. cellulolyticum physiology with cellobiose, as a model of soluble cellodextrin, and with pure cellulose, as a carbon source more closely related to lignocellulosic compounds, strengthen the idea of a bacterium particularly well adapted, and even restricted, to a cellulolytic lifestyle. The metabolic flux analysis from continuous cultures revealed that (i) in comparison to cellobiose, the cellulose hydrolysis by the cellulosome introduces an extra regulation of entering carbon flow resulting in globally lower metabolic fluxes on cellulose than on cellobiose, (ii) the glucose 1-phosphate/glucose 6-phosphate branch point controls the carbon flow directed towards glycolysis and dissipates carbon excess towards the formation of cellodextrins, glycogen and exopolysaccharides, (iii) the pyruvate/acetyl-CoA metabolic node is essential to the regulation of electronic and energetic fluxes. This in-depth analysis of C. cellulolyticum metabolism has permitted the first attempt to engineer metabolically a cellulolytic microorganism.

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Adhesive properties of five mesophilic, cellulolytic Clostridia isolated from the same biotope

Cited by 2 publications

References 7 publications

Newspaper as a substrate for cellulolytic landfill bacteria

Newspaper as a substrate for cellulolytic landfill bacteria

Clostridium cellulolyticum: model organism of mesophilic cellulolytic clostridia

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