Adhesive Growth of Pancreatic Islet Cells on a Polyglycolic Acid Fibrous Scaffold

Song, Chao; Huang, Yudong; Xie, Wan; Hou, Yan; Huang, R.P.; Song, Yimin; Liu, X.M.; Zheng, Weiyan; Shi, Yanlin

doi:10.1016/j.transproceed.2008.02.088

Cited by 25 publications

(30 citation statements)

References 13 publications

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“…For example, rat islets cultured in a porous poly(glycolic acid) (PGA) scaffold were nearly twofold more viable and had fourfold greater insulin secretion when compared to those cultured on untreated TCPS in 2D after 15 days. 96 In a separate study, human islets were entrapped in a collagen gel containing fibronectin and collagen IV within the pores of a poly(lactic acid-co-glycolic acid) (PLGA) scaffold. 97 Interestingly, the islets within the PLGA scaffold maintained a glucose stimulation index similar to that of fresh islets and better than those cultured in a 3D collagen I gel, but in the absence of the scaffold, or those cultured in suspension.…”

Section: Three-dimensional Porous Scaffoldsmentioning

confidence: 99%

Tissue Engineering Approaches to Cell-Based Type 1 Diabetes Therapy

Amer

Mahoney

Bryant

2014

Tissue Engineering Part B: Reviews

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Type 1 diabetes mellitus is an autoimmune disease resulting from the destruction of insulin-producing pancreatic b-cells. Cell-based therapies, involving the transplantation of functional b-cells into diabetic patients, have been explored as a potential long-term treatment for this condition; however, success is limited. A tissue engineering approach of culturing insulin-producing cells with extracellular matrix (ECM) molecules in threedimensional (3D) constructs has the potential to enhance the efficacy of cell-based therapies for diabetes. When cultured in 3D environments, insulin-producing cells are often more viable and secrete more insulin than those in two dimensions. The addition of ECM molecules to the culture environments, depending on the specific type of molecule, can further enhance the viability and insulin secretion. This review addresses the different cell sources that can be utilized as b-cell replacements, the essential ECM molecules for the survival of these cells, and the 3D culture techniques that have been used to benefit cell function.

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Section: Three-dimensional Porous Scaffoldsmentioning

confidence: 99%

Tissue Engineering Approaches to Cell-Based Type 1 Diabetes Therapy

Amer

Mahoney

Bryant

2014

Tissue Engineering Part B: Reviews

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“…12 Three-dimensional (3D) culture is crucial for mimicking the role of native ECM, which could be achieved with biodegradable polymeric scaffolds thereby offering long-term islet survival and function. [13][14][15][16] The present study utilizes the principles of tissue engineering via combination of cells, biomaterials, and growth factors to generate insulin-producing biohybrid pancreatic construct. The scaffold of study named as dextran and gelatin (DEXGEL) has an open interconnected macroporous nature fabricated by in situ cross-linking of two components namely gelatin and dextran dialdehyde (DDA) followed by a freeze-drying process.…”

Section: Introductionmentioning

confidence: 99%

Enhanced Survival and Function of Islet-Like Clusters Differentiated from Adipose Stem Cells on a Three-Dimensional Natural Polymeric Scaffold: AnIn VitroStudy

AloysiousNeena

NairPrabha

2014

Tissue Engineering Part A

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Autologous adipose stem cells owing to its pluripotent nature offer a valuable source for pancreatic beta cell replacement in the treatment of diabetes mellitus. However, maintaining longevity and functionality of stem cell-derived islet-like cells for long-term in vitro culture is challenging. Signaling interaction between islets and surrounding extracellular matrix (ECM) is an important factor for islet survival and function. Tissue engineering strategy to use scaffolds as substitute for ECM is a key to the problem. In the present study, we fabricated a three-dimensional (3D) biodegradable scaffold comprised of natural polymers dextran and gelatin (DEXGEL) for differentiation of adipose stem cells to islet-like clusters (ILCs). Adipose stem cells derived from subcutaneous fat of New Zealand white rabbits were differentiated to ILCs on DEXGEL scaffold and two-dimensional (2D) culture plates via three stage protocol using cocktail of growth factors. The ILCs differentiated on DEXGEL scaffold exhibited characteristic islet morphology, and expressed islet-specific hormones (insulin, glucagon, and somatostatin). The insulin secretion in response to glucose challenge and viability of ILCs on DEXGEL scaffold were significantly higher in comparison to ILCs on 2D culture. Our results demonstrated for the first time that DEXGEL scaffold simulated an extracellular environment for effective differentiation of rabbit adipose stem cells to ILCs.

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“…To improve the yield of pure islets, in addition to collagenase, the methods included Ficoll or accutase. Ficoll solutions allow gradient centrifugation of pancreatic islets and improve the retrieval of pure islets in humans and rodents, following collagenase digestion (Chun et al, 2008). Accutase is an enzymatic solution extracted from an invertebrate species containing collagenases and other proteases, and employed to isolate pancreatic islets or, more commonly, to separate β-cells after the islets have been isolated from human and rodent pancreas (Toyama et al, 2003).…”

mentioning

confidence: 99%