Adhesive and robust multilayered poly(lactic acid) nanosheets for hemostatic dressing in liver injury model

Komachi, Takuya; Sumiyoshi, Hideaki; Inagaki, Yutaka; Takeoka, Shinji; Yu, Na; Okamura, Yosuke

doi:10.1002/jbm.b.33714

Cited by 24 publications

(29 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the field of biomaterials, polymer thin films with a thickness in the order of 10–100 nm, which are known as nanosheets, have been proposed as novel materials for surgical application ( Okamura et al., 2009 , 2013 ; Fujie et al., 2010 ; Hagisawa et al., 2013 ; Komachi et al., 2017 ) and bioimaging ( Zhang et al., 2017 , 2018b ; Someya et al., 2018 ). Nanosheets have unique properties, including high adhesion strength, flexibility, and transparency.…”

Section: Introductionmentioning

confidence: 99%

PEO-CYTOP Fluoropolymer Nanosheets as a Novel Open-Skull Window for Imaging of the Living Mouse Brain

Takahashi,

Zhang,

Kawakami

et al. 2020

iScience

Self Cite

View full text Add to dashboard Cite

Summary In vivo two-photon deep imaging with a broad field of view has revealed functional connectivity among brain regions. Here, we developed a novel observation method that utilizes a polyethylene-oxide-coated CYTOP (PEO-CYTOP) nanosheet with a thickness of ∼130 nm that exhibited a water retention effect and a hydrophilized adhesive surface. PEO-CYTOP nanosheets firmly adhered to brain surfaces, which suppressed bleeding from superficial veins. By taking advantage of the excellent optical properties of PEO-CYTOP nanosheets, we performed in vivo deep imaging in mouse brains at high resolution. Moreover, PEO-CYTOP nanosheets enabled to prepare large cranial windows, achieving in vivo imaging of neural structure and Ca 2+ elevation in a large field of view. Furthermore, the PEO-CYTOP nanosheets functioned as a sealing material, even after the removal of the dura. These results indicate that this method would be suitable for the investigation of neural functions that are composed of interactions among multiple regions.

show abstract

Section: Introductionmentioning

confidence: 99%

PEO-CYTOP Fluoropolymer Nanosheets as a Novel Open-Skull Window for Imaging of the Living Mouse Brain

Takahashi,

Zhang,

Kawakami

et al. 2020

iScience

Self Cite

View full text Add to dashboard Cite

show abstract

“…A previous study demonstrates that an extracellular matrix (ECM) patch graft contributes to repairing defects in the stomach (Hussey, Cramer, & Badylak, ), but the concept of a patch is merely applied to the surface area of gastrointestinal tract, meaning it cannot thoroughly act on all internal damaged hepatocytes, as resulted from inflammation of the liver. Moreover, a Polylactic acid (PLA)‐based nanosheets were fabricated for only hemostatic dressing in liver injury model without promoting liver regeneration (Komachi et al, ). Therefore, this study proposes a novel hypothesis; if a biomaterial can be injected via the hepatic portal vein into a damaged liver to act on the hepatocytes, it can comprehensively repair the damaged hepatocytes, thus, it may be an effective therapeutic method for patients with liver fibrosis or even liver cirrhosis.…”

Section: Introductionmentioning

confidence: 99%

Decellularized liver matrix as substrates for rescue of acute hepatocytes toxicity

2019

View full text Add to dashboard Cite

More and more scholars regard the lesion of liver cirrhosis as a series of progressive clinical stages. Besides, liver cirrhosis is a late stage of scarring (fibrosis) of the liver, and has long been a common cause of death for the global adult population, thus, the treatment of liver cirrhosis is a key point investigated in the biomedical field. Here, we propose a novel hypothesis; if decellularized liver matrix (DLM) is possible injected into the injurant‐induced fiberized liver via the hepatic portal vein to thoroughly repair the liver, it may be an effective therapeutic method for liver fibrosis or even liver cirrhosis. This study mixed rat DLM with gelatin‐hydroxyphenylpropionic acid (Glt‐HPA) to form a three‐dimensional structure to simulate the in vivo liver environment, and cultured the primary rat hepatocytes in it. Afterward, the hepatocytes were treated using D‐galactosamine (GaIN), CHCl3, and CCl4‐containing medium to simulate the toxin‐mediated liver fibrosis in vitro. Finally, they were cultured in a DLM‐containing medium to observe the viability and functions of the damaged hepatocytes, and the hypothesis of this research was proved, meaning that Glt‐HPA‐DLM acting on damaged hepatocytes may repair them. Results have shown that Glt‐HPA‐DLM was effective for hepatocytes culture and repaired injured hepatocytes from GaIN, CHCl3, and CCl4 (albumin synthesis was increased by 219, 108, and 12%, respectively, whereas relative lactate dehydrogenase activity was reduced by 38, 68, and 67%, after 5 days of culture, separately). This research shows promising effects against hepatic fibrosis and may have potential for liver cirrhosis in vivo.

show abstract

“…Using a spin-coating method, the thickness of CYTOP nanosheet is adjustable and is set to be 130 nm in this study (S1 Fig). A water-soluble polymer layer is coated before CYTOP casting, and nanosheet can then float on the surface of water with dissolution of the underlying layer [9][10][11][12][13][14]. Nanosheet wrapping on a tissue specimen is accomplished with the help of a wire loop, while the marginal part of the nanosheet can automatically adhere to any surface (Fig 2).…”

Section: Resultsmentioning

confidence: 99%

Nanosheet wrapping-assisted coverslip-free imaging for looking deeper into a tissue at high resolution

et al. 2020

Self Cite

View full text Add to dashboard Cite

In order to achieve deep tissue imaging, a number of optical clearing agents have been developed. However, in a conventional microscopy setup, an objective lens can only be moved until it is in contact with a coverslip, which restricts the maximum focusing depth into a cleared tissue specimen. Until now, it is still a fact that the working distance of a high magnification objective lens with a high numerical aperture is always about 100 μm. In this study, a polymer thin film (also called as nanosheet) composed of fluoropolymer with a thickness of 130 nm, less than one-thousandth that of a 170 μm thick coverslip, is employed to replace the coverslip. Owing to its excellent characteristics, such as high optical transparency, mechanical robustness, chemical resistance, and water retention ability, nanosheet is uniquely capable of providing a coverslip-free imaging. By wrapping the tissue specimen with a nanosheet, an extra distance of 170 μm for the movement of objective lens is obtained. Results show an equivalently high resolution imaging can be obtained if a homogenous refractive index between immersion liquid and mounting media is adjusted. This method will facilitate a variety of imaging tasks with off-the-shelf high magnification objectives.

show abstract

Adhesive and robust multilayered poly(lactic acid) nanosheets for hemostatic dressing in liver injury model

Cited by 24 publications

References 40 publications

PEO-CYTOP Fluoropolymer Nanosheets as a Novel Open-Skull Window for Imaging of the Living Mouse Brain

PEO-CYTOP Fluoropolymer Nanosheets as a Novel Open-Skull Window for Imaging of the Living Mouse Brain

Decellularized liver matrix as substrates for rescue of acute hepatocytes toxicity

Nanosheet wrapping-assisted coverslip-free imaging for looking deeper into a tissue at high resolution

Contact Info

Product

Resources

About