Adhesion to and invasion of HEp-2 cells by Campylobacter spp

Abstract

Twenty-one isolates were tested for their ability to adhere to and invade HEp-2 cells in vitro. Of the 21 organisms tested, 2 did not invade the HEp-2 cells, and 1 of these did not adhere to the epithelial cells. Campylobacterjejuni clinical isolates were more invasive than the nonclinical strains that were tested. When HEp-2 cells were treated with cytochalasin B, the invasiveness of C. jejuni was reduced, indicating active participation of the host cell in the uptake of these organisms. The number of intracellular C. jejuni isolates decreased when Campylobacter whole-cell lysates were adsorbed onto HEp-2 cell monolayers. Experiments were also conducted to identify the functional sites of the antigens responsible for expression of Campylobacter invasion. Oxidation of lysates with sodium meta-periodate significantly affected its inhibitory capacity. This implies that the Campylobacter invasive ligand appears to be dependent upon an intact carbohydrate moiety.

A major role for intestinal epithelial nucleotide oligomerization domain 1 (NOD1) in eliciting host bactericidal immune responses to Campylobacter jejuni

“…Intestinal epithelial NOD1 and NOD2 gene expression is not modulated in response to C. jejuni Bacterial adherence to and subsequent invasion of the IEC is a well established early event in C. jejunimediated disease pathogenesis (Konkel and Joens, 1989;Szymanski et al, 1995;Monteville et al, 2003).…”

Uptake pathways of clinical and healthy animal isolates of Campylobacter jejuni into INT-407 cells

“…The adherence and invasion assays were performed by the method of Konkel and Joens [21] with some modi¢cation. Brie£y, 100 Wl of bacterial suspension, containing CFU approximately 100 times higher than cell number, was inoculated into duplicate wells of a 24-well tissue culture plate containing semicon£uent monolayers of INT-407 cells.…”

Isolation of motile and non‐motile insertional mutants of Campylobacter jejuni: the role of motility in adherence and invasion of eukaryotic cells

“…However. C. jejuni and 0. coli have recently been shown to be naturally transformable (Wang and Taylor, 1990), although this observation has been only of limited use in terms of genetic exploifation of the genus, Campylobaoter diarrhoea most often presents with symptoms typical of an invasive disease (Skirrow and Blaser, 1992), Consistent with this, several kiboratories have reported that some Campylobacter strains can invade a variety of eukaryotic cells in culture at relatively low levels by a microfilament (MF)-dependent mechanism (Konkei and Joens. 1989;Konkel et ai, 1992); more recently, a higher level microtubule (MT)-dependent system has been described for other strains, including C. jejuni 8^-^76 (Oelschlaegor et ai.…”