Adhesion sites of neural tumor cells: biochemical composition

Culp, Lloyd A.; Ansbacher, Riva; Domen, Constance

doi:10.1021/bi00566a036

Cited by 56 publications

(22 citation statements)

References 37 publications

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“…The formation of hyaluronate-containing cell coats is consistent with previous reports of hyaluronic acid synthesis in rat and human glioma lines in vitro (24)(25)(26). In addition, there is histochemical evidence for the existence of mucosubstances in tumor biopsy specimen of both intracranial gliomas and optic nerve gliomas (27)(28)(29)(30).…”

Section: Discussionsupporting

confidence: 91%

Ultrastructural features of the lymphocyte-stimulated halos produced by human glioma-derived cells in vitro

Oberc-Greenwood¹,

Muul²,

Gately³

et al. 1986

J Neuro-Oncol

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Many glioma-derived cell lines have the capability of escaping cell-mediated immune attack. One mechanism of escape is the secretion of a hyaluronidase-sensitive mucopolysaccharide coat by these cells. This coat prevents contact and tumor cell killing by specific cytolytic allogeneic lymphocytes. The production of the coat by the tumor cells is stimulated by a macromolecular factor released by peripheral blood mononuclear (PBMC) cells in culture. We have examined the morphologic and ultrastructural features of this extracellular matrix. Three coat-producing lines were studied. Under phase contrast light microscopy, the coat is a clear pericellular 'halo'. To stain this zone, ruthenium red and Alcian Blue 8 G stains, which bind to acid mucopolysaccharides (to a large extent, hyaluronic acid), were used. The two stains produced similar results. With light microscopy, a weblike pattern of stain was evident throughout the halo region. With transmission electron microscopy, staining was found along the plasma membrane of the glioma cells and their microvilli, stretching in long, branching filaments from these surfaces and, in some instances, from one microvillus to the next. Since mucopolysaccharide matrices have a large aqueous component, it was necessary to determine whether dehydration alters the stain pattern. Therefore, undehydrated ruthenium red stained specimens from each culture were embedded in Quetal 651 (Ted Pella, Inc., Tustin, CA), a water soluble plastic. No morphologic differences were noted between the hydrated and dehydrated specimens. This study indicates that numerous long microvilli and a secreted mucopolysaccharide matrix are important structural elements of the lymphocyte-stimulated tumor cell halo in vitro. The mechanism by which the PBMC factor stimulates coat formation and the importance of the coat in in vivo tumor defenses remain to be elucidated.

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Section: Discussionsupporting

confidence: 91%

Ultrastructural features of the lymphocyte-stimulated halos produced by human glioma-derived cells in vitro

Oberc-Greenwood¹,

Muul²,

Gately³

et al. 1986

J Neuro-Oncol

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“…Heparan sulfate-proteoglycans may also function in the control of cell motility and adhesion, since heparan sulfate is present in the newly formed adhesion sites of fibroblasts , glioma cells, and neuroblastoma cells (e.g., Culp et al 1979Culp et al , 1980. Heparinase digestion of the cell surface prevents the !…”

Section: Discussionmentioning

confidence: 99%

Studies on Extracellular Matrix Components That Promote Neurite Outgrowth

Lander¹,

Tomaselli²,

Calof³

et al. 1983

Cold Spring Harbor Symposia on Quantitative Biology

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“…Tumor metasis is one of the more important unsolved biological phenomena in which the cell surface plays an important role [3,61. The significance of plasma membrane carbohydrates in the metastasis of malignant B16 melanoma cells is unknown.…”

Section: Discussionmentioning

confidence: 99%

The role of glycoconjugates in metastatic melanoma blood‐borne arrest and cell surface properties

Irimura

Nicolson

1981

J. Supramol. Struct. Cell. Biochem.

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The role of glycoconjugates in cell surface and blood-borne implantation properties of murine metastatic melanoma sublines of low (B16-F1) or high (B16-F10) potential to colonize lungs was investigated by treating melanoma cells with the antibiotic tunicamycin. This drug prevents glycosylation of glycoproteins by inhibiting the formation of lipid-linked oligosaccharide precursors. The degree of tunicamycin-mediated modifications in glycoproteins was assessed by monitoring the decrease in cell surface sialogalactoproteins by binding of 125I-labeled Ricinus communis agglutinin I. Scanning electron microscopy of tunicamycin-treated B16-F1 and B16-F10 cells showed morphologic changes such as cell rounding and formation of numerous surface blebs. Tunicamycin-treated B16-F1 and B16-F10 cells lost their lung colonization abilities when injected intravenously into C57BL/6 mice, concomitant with lowered rates of adhesion to endothelial cell monolayers, endothelial extracellular matrix (basal lamina), and polyvinyl-immobilized fibronectin in vitro, suggesting that this drug inhibits experimental metastasis by modifying the surface glycoproteins involved in determining the adhesive properties of malignant cells.

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Adhesion sites of neural tumor cells: biochemical composition

Cited by 56 publications

References 37 publications

Ultrastructural features of the lymphocyte-stimulated halos produced by human glioma-derived cells in vitro

Ultrastructural features of the lymphocyte-stimulated halos produced by human glioma-derived cells in vitro

Studies on Extracellular Matrix Components That Promote Neurite Outgrowth

The role of glycoconjugates in metastatic melanoma blood‐borne arrest and cell surface properties

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