Adhesion receptors in malignant transformation and dissemination of gastrointestinal tumors

Streit, Michael; Schmidt, R; Hilgenfeld, R. U.; Thiel, E.; Kreuser, E. D.

doi:10.1007/s001090050027

Cited by 21 publications

(33 citation statements)

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“…Abnormalities of E-cadherin expression have been described in colorectal carcinoma [28] and have been correlated with increased invasiveness, metastatic potential and poor patient prognosis in this tumor type [29] . Recent research showed that E-cadherin expression was significantly lower in the de novo group tissue than that in the prudent group [30,31] . In the present study, by Western blotting analysis, we also confirmed that LST-R1 cells have a prominent lower expression of E-cadherin than that in several other cell lines.…”

Section: Discussionmentioning

confidence: 96%

“…Twenty-four hours later, the numbers of LST-R1 cells invading the membrane were significantly higher (179.3 ± 40 cells/well) than those of lovo (139.3 ± Table 1. [30],-3 [9],der(3)del(p12)t(2;3)(q21;q11) [21],-3 [9],der(4)t(4;10)(q11;p11) [30],d er(4)dup(14)(q22q31)t(4;14)(q?;q?) [9],der(4)t(3;4)(p21;p16)dup(14)(q22 q31)t(4;14)(q?;q?)…”

Section: The Invasion Ability Of Lst-r1 In Vitromentioning

confidence: 99%

“…[21],-4 [30],der(5;8)(p10;p10) [30],+i(5)(p10)×2 [30],+der (5;20)(p10;q10)del(5)(q31) [30],der(6)t(2:6)(p13;q23) [30],der(7)t(7;15)(p2 2;q?) [30],der(7)t(7;17)(p13;?) [29],der(8)(3;8)(?…”

Section: The Invasion Ability Of Lst-r1 In Vitrounclassified

“…;q22) [30],der(9)t(9;19)(p1 1;?) [30],+der(9)t(9;19)(q11;?) [30],+r(9)(p13q13) [29],der(10;13)(q10;q10) [30],der(10)t(4;10)(p11;11)t(4;8)(q31;q13)t(3;10)(p23;q23) [30],der(12)t(3: 12)(q24;q11) [30],der(12)t(5;12)(?…”

Section: The Invasion Ability Of Lst-r1 In Vitrounclassified

“…[30],+der(9)t(9;19)(q11;?) [30],+r(9)(p13q13) [29],der(10;13)(q10;q10) [30],der(10)t(4;10)(p11;11)t(4;8)(q31;q13)t(3;10)(p23;q23) [30],der(12)t(3: 12)(q24;q11) [30],der(12)t(5;12)(? ;p11) [30],+der(12)t(2;12)(q22;q11) [30], +der(12)t(2;12)(p?;p11)t(2;12)(q24;q?)…”

Section: The Invasion Ability Of Lst-r1 In Vitrounclassified

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Establishment and characterization of a new cell line derived from human colorectal laterally spreading tumor

Wang¹,

Lai²,

Yeung³

et al. 2008

WJG

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CONCLUSION:We established and characterized a colorectal cancer cell line, LST-R1 and LST-R1 has an obvious malignant tendency, which maybe partially attributed to the changes of the expression of some adhesion molecules, such as E-cadherin. It is also a versatile tool for exploring the original and progressive mechanisms of laterally spreading tumor and the early colon cancer genesis. INTRODUCTIONTwo routes have been described that can lead to colon carcinogenesis: one is the adenoma-carcinoma model, which is the main carcinogenetic pathway of prudent colorectal tumor and has been widely accepted in the west. However, an increasing number of superficial or flat colorectal tumors have been reported, particular in Japan [1,2] . The superficial tumors are considered to have a distinct clinicopathologic, genetic feature and behave a different carcinogenetic pathway, called de novo pathway [3][4][5][6] . Laterally spreading tumor (LST) is a unique subtype of superficial colorectal tumor, which was first reported by Kudo S in 1993 [7,8] and many names such as granular cluster type, nodule-aggregating tumor, creeping tumor, superficial spreading (epithelial) tumor, flower-bed-like lesion had been used. In 1998, it was defined as tumors originated from the colorectal mucous membranes and mainly extends laterally rather than vertically with its diameter greater than 1 cm ( Figure 1A) [9][10][11][12] . It has a tendency to affect the rectum, sigmoid colon, and caecum. In some studies, it has been reported that the carcinogenesis rate of LST is 8.64%-52.5% [13][14][15] . Abstract AIM: To study the molecular mechanism of laterally spreading tumor (LST), a cell line [Laterally Spreading Tumor-Rectum 1 (LST-R1)] was derived and the characteristics of this cell line were investigated. BASIC RESEARCH METHODS:A new cell line (LST-R1) originated from laterally spreading tumor was established. Properties of the cell line were characterized using scanning and transmission electron microscopy, immunohistochemistry method, cytogenetic analysis and nude mice xenograft experiments. In vitro invasion assay, cDNA microarray and Western blotting were used to compare the difference between the LST-R1 and other colorectal cancer cell lines derived from prudent colon cancer. RESULTS:Our study demonstrated that both epithelial special antigen (ESA) and cytokeratin-20 (CK20) were expressed in LST-R1. The cells presented microvilli and tight junction with large nuclei. The karyotypic analysis showed hyperdiploid features with structural chromosome aberrations. The in vivo tumorigenicity was also demonstrated in nude mice xenograft experiments. The invasion assay suggested this cell line has a higher invasive ability. cDNA microarray and Western blotting show the loss of the expression of E-cadherin in LST-R1 cells.

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Section: Discussionmentioning

confidence: 96%

Section: The Invasion Ability Of Lst-r1 In Vitromentioning

confidence: 99%

“…[21],-4 [30],der(5;8)(p10;p10) [30],+i(5)(p10)×2 [30],+der (5;20)(p10;q10)del(5)(q31) [30],der(6)t(2:6)(p13;q23) [30],der(7)t(7;15)(p2 2;q?) [30],der(7)t(7;17)(p13;?) [29],der(8)(3;8)(?…”

Section: The Invasion Ability Of Lst-r1 In Vitrounclassified

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Establishment and characterization of a new cell line derived from human colorectal laterally spreading tumor

Wang¹,

Lai²,

Yeung³

et al. 2008

WJG

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What can we learn from the phenomenon of preferential lymph node metastasis in carcinoma?

Gendreau

Whalen

1999

J. Surg. Oncol.

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Inhibition of constitutive NF‐κB activity suppresses tumorigenicity of ewing sarcoma EW7 cells

Javelaud

Poupon

Besançon

2001

Intl Journal of Cancer

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Ewing sarcoma is 1 of the most aggressive tumors that can affect children and young adults. Despite advances in therapy, the prognosis remains poor emphasizing the need for defining new targets for treatment. We investigated a possible role of nuclear factor-B (NF-B) activity of Ewing sarcoma-derived EW7 cells in their tumorigenicity. In these cells, expression of a degradation-resistant form of the inhibitory factor IB␣ inhibited NF-B activity without affecting their in vitro proliferation rate. It causes, however, a remarkable loss of their ability to generate tumors in nude mice that correlates with both a decrease in extracellular matrix (ECM) protein secretion and an acquisition of sensitivity to murine tumor necrosis factor ␣ (TNF␣)-induced apoptosis. These data support the concept that NF-B activity plays a role in the tumorigenicity of Ewing sarcoma cells, identifying NF-B as a potential target for reducing Ewing tumor progression.

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Adhesion receptors in malignant transformation and dissemination of gastrointestinal tumors

Cited by 21 publications

References 0 publications

Establishment and characterization of a new cell line derived from human colorectal laterally spreading tumor

Establishment and characterization of a new cell line derived from human colorectal laterally spreading tumor

What can we learn from the phenomenon of preferential lymph node metastasis in carcinoma?

Inhibition of constitutive NF‐κB activity suppresses tumorigenicity of ewing sarcoma EW7 cells

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