Adhesion receptor expression by hematopoietic cell lines and murine progenitors

Becker, Pamela S.; Nilsson, Susan K.; Li, Zhifang; Berrios, Virla M.; Dooner, Mark S.; Cooper, Cathleen L.; Hsieh, Chung Cheng; Quesenberry, Peter J.

doi:10.1016/s0301-472x(98)00037-x

Cited by 131 publications

(95 citation statements)

References 35 publications

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“…These categories included transcription factors (22), protein synthesis regulators (11), surface proteins (11), mitochondrial sequences (10), RNA metabolism proteins (10), signaling pathway factors (9), and cytokines (8). In separate studies, we have also shown that Lin Ϫ Rho dull Ho dull marrow cells express adhesion proteins 48,49 and cytokine receptors 50 on the cell surface. These data indicate a different picture of the primitive marrow stem cell than is usually envisioned-a functional cell in which a large number of stem cell-specific, as opposed to differentiated, functions are manifest (Figure 2).…”

Section: Stem Cell Genes and Functionsmentioning

confidence: 85%

“…73,74 Studies of adhesion protein cell-surface expression on Lin Ϫ Rho dull Ho dull and Lin Ϫ Sca-1 ϩ hematopoietic stem cells with cell cycle transit showed fluctuations of ␣-4, ␣-5, ␣-6, ␤-1, L-selectin, and platelet-endothelial cell adhesion molecule-1 (PECAM-1) at different points in the cell cycle. 48,49 A probable causal role for these fluctuations in the engraftment fluctuations was shown by homing studies in which it was found that CFDA-SE (5-(and -6)-carboxyfluorescein diacetate succinimidyl ester)-labeled Lin Ϫ Sca ϩ stem cells cultured in IL-3, IL-6, IL-11, and steel factor showed a marked depression in homing at a time when engraftment and ␣-4 were also markedly depressed (48 hours of culture). 75 A schematic summarizing the results with marrow cells cultured in IL-3, IL-6, IL-11, and steel factor is shown in Figure 4.…”

Section: Changing Engraftable Multipotent Stem Cell Phenotype With Cementioning

confidence: 99%

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The chiaroscuro stem cell: a unified stem cell theory

Quesenberry

Colvin

Lambert

2002

Blood

120

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Hematopoiesis has been considered hierarchical in nature, but recent data suggest that the system is not hierarchical and is, in fact, quite functionally plastic. Existing data indicate that engraftment and progenitor phenotypes vary inversely with cell cycle transit and that gene expression also varies widely. These observations suggest that there is no progenitor/stem cell hierarchy, but rather a reversible continuum. This may, in turn, be dependent on shifting chromatin and gene expression with cell cycle transit. If the phenotype of these primitive marrow cells changes from engraftable stem cell to progenitor and back to engraftable stem cell with cycle transit, then this suggests that the identity of the engraftable stem cell may be partially masked in nonsynchronized marrow cell popula- IntroductionWe present here a different view of early hematopoiesis. This is not the standard dogma of many investigators in the field, but is built upon solid work by many laboratories over many years. This is a perspective, and thus naturally reflects the biases of the authors. These, in turn, have a basis in many published studies from our laboratory and other laboratories and also from extensive unpublished, but abstracted, data from our laboratory. It is appropriate to consider this contribution as speculative in nature.The term chiaroscuro refers to the treatment of light and shade in painting. Our current view of the changing phenotype of the marrow stem cell suggests a chiaroscuro nature to this picture.In general, models of stem cell regulation have been hierarchical. 1,2 A primitive stem cell, with great potential, gives rise to a proliferating progenitor pool, which in turn gives rise to recognizable differentiated cells. During this process, proliferative potential is lost, while specific differentiated features are acquired. Presumptively, but without definite proof, there is also self-renewal at the most primitive stem cell level, and this is also lost with differentiation. Many data exist to support such a hierarchical model. Marrow cells have been separated with short-and long-term repopulation potential 3,4 and progenitors have been characterized as exclusively committed to the production of restricted progeny. 5,6 In addition, the clear expansion of different progenitor types in cytokinestimulated in vitro culture with a loss of long-term engraftment capacity speaks to the existence of a progenitor hierarchy, at least at the more differentiated progenitor levels. 7,8 A model encompassing these features is shown in Figure 1.This model does not fit with all published data. For instance, the "daughter cell" or paired-progenitor experiments of Suda and colleagues 9 indicate that a primitive progenitor cell can make totally different lineage choices during one cell cycle transit, such that, for example, one daughter cell forms an erythroid colony while the other daughter (or sister) cell forms a neutrophilmacrophage colony. Out of a total of 387 pairs evaluated, 68 pairs of colonies consisted of dissimilar com...

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Section: Stem Cell Genes and Functionsmentioning

confidence: 85%

Section: Changing Engraftable Multipotent Stem Cell Phenotype With Cementioning

confidence: 99%

The chiaroscuro stem cell: a unified stem cell theory

Quesenberry

Colvin

Lambert

2002

Blood

120

View full text Add to dashboard Cite

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“…Using syngeneic inbred mice, many aspects of stem cell homing have been described (Vos et al, 1980;Visser and Eliason, 1983;Bertoncello et al, 1985;Tavassoli and Aizawa, 1987;Hardy et al, 1991;Hendrikx et al, 1996;Nilsson et al, 1996;Nibley et al, 1997;Shaaban et al, 1999;Lanzkron et al, 1999a;Askenasy et al, 2003). The process involves rapid phenotypic changes in the infused stem cells and the interactions of multiple adhesion protein and their receptors (Papayannopoulou and Craddock, 1997;Frenette et al, 1998;Becker et al, 1999;Lapidot et al, 2005). Marrow stem cells enter S phase within about 12 h after infusion in vivo.…”

mentioning

confidence: 99%

Murine allogeneic in vivo stem cell homing^,

Colvin

Lambert²,

Dooner

et al. 2006

Journal Cellular Physiology

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Stem cell homing has been studied in syngeneic models and appears to be rapid (<1 h) and dependent on cellular adhesion and migration factors. We utilized a full H2-mismatched transplantation model to determine the basics of allogeneic homing. C57BL/6J Lin-Sca-1+ cells were labeled with CFSE and injected in non-myeloablated BALB/c mice. Fluorescent cell detection was via high-speed FACS analysis. Alternatively, B6.SJL whole bone marrow cells were injected in lethally irradiated BALB/ c mice (10 Gy). One, 3, 6, and 24 h after transplant, marrow was harvested and cells were either plated for high proliferative potential colony-forming cell (HPP-CFC) assay or secondarily injected into myeloablated (8 Gy) C57BL/6J mice using 10% competing C57BL/6J marrow. Chimerism was evaluated at 8 weeks. CFSE+ cells were detected in the bone marrow 1, 3, and 6 h after injection. The numbers were moderately lower when compared to syngeneic homing possibly due to strain effect. Conversely, utilizing a surrogate or secondary assay, we observed a decline of secondary engraftment of harvested cells over time, but not of HPP-CFC. Combining experiments and normalizing the 1-h time point to 100% (to allow comparison), we observed a mean relative engraftment of 87 ± 29%, 72 ± 21%, 84 ± 35% of the 1 h level at 3, 6, and 24 h respectively. HPP-CFC assay showed no significant variation as a homing surrogate over 1-6 h. These data indicate a rapid homing into allogeneic recipients with a plateau at 1 h. The decline of secondary engraftability over time may indicate a phenotype alteration of homed cells.Hematopoetic stem cells (HSC) are defined by their capacity to fully reconstitute marrow of lethally irradiated hosts and give rise to all marrow-derived hematopoietic elements. Stem cell transplantation is a clinical approach used to restore defective hematopoiesis due either to highdose chemoradiotherapy or to intrinsic marrow diseases. In this process, intravenously infused stem cells rapidly find their way to specific periendosteal location in the marrow-termed niches. The totality of this process defines homing . Using syngeneic inbred mice, many aspects of stem cell homing have been described (Vos et al

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“…7 The quiescent stage of CD34 + cells is important because induction of cell proliferation is associated with a loss of the potential to reconstitute hematopoiesis and with changes in the expression of cellular receptors. [8][9][10] In this regard, application of retroviruses to HSCs is limited, because efficient gene transfer is only seen in actively replicating target cells. In a retrovirus system, the fraction of dividing cells needs to be expanded before transduction by prestimulation with cytokines in order to increase the transduction efficiency of HSCs up to 40%.…”

Section: Discussionmentioning

confidence: 99%