Adhesion receptor CD11b/CD18 contributes to neutrophil diapedesis across the bovine blood–milk barrier

Smits, Elke; Burvenich, Christian; Guidry, A.J.; Massart-Leën, Annemie

doi:10.1016/s0165-2427(00)00157-4

Cited by 32 publications

(32 citation statements)

References 33 publications

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“…Indeed, LPS directly or indirectly [10] induces adherence of circulating PMN to the endothelium by the upregulation of CD11b/CD18 [30]. The CD11b/CD 18 molecule is crucial to bovine PMN diapedesis across the blood/milk barrier [36]. In addition, the increased concentrations of BSA in milk from challenged quarters suggest an increased permeability of the blood/milk barrier.…”

Section: Discussionmentioning

confidence: 99%

Local and systemic effects of endotoxin mastitis on the chemiluminescence of milk and blood neutrophils in dairy cows

et al. 2001

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-The local and systemic effects of intramammary lipopolysaccharide (LPS) injection on the chemiluminescence (CL) of milk and blood polymorphonuclear leukocytes (PMN) were investigated in six healthy early lactation cows. Clinical signs of acute mastitis such as fever, increased heart rate and a decreased milk production were observed in all cows. Before LPS challenge, the CL activity of milk PMN was significantly lower than that of blood PMN (P < 0.01). A significant negative correlation was found between pre-challenge milk and blood PMN CL and, the decreased milk production in unchallenged quarters. The CL activity of milk PMN from LPS-injected quarters increased following LPS challenge, whereas it remained unchanged in control quarters. The CL activity of blood PMN showed a biphasic increase, with two peaks and a valley below pre-challenge CL activity (P < 0.01). At post-challenge hours (PCH) 6 and 12, the CL activity of milk PMN from LPS-injected quarters exceeded that of blood PMN (P < 0.05 and P < 0.001, respectively). The decreased CL activity of blood PMN and the enhanced CL activity of milk PMN during endotoxin-induced mastitis was reflected by changes in the shape of the CL curve. In blood PMN, a decrease of the second peak of the CL curve suggests that the myeloperoxidase (MPO)-H 2 O 2 system is impaired during endotoxin-induced mastitis. In contrast, the MPO-H 2 O 2 system was enhanced in milk PMN from challenged quarters. The highest duration and intensity of reactive oxygen intermediate (ROI) production was observed in milk PMN from LPS-injected quarters at PCH 12. The increased viability of PMN in LPS-injected quarters and to a lesser extent in control quarters suggests possible effects of both facilitated diapedesis and inflammatory mediators on milk PMN survival. In conclusion, our results suggest that a combination of local and systemic action of E. coli endotoxin is involved in the priming of milk PMN during mastitis. chemiluminescence / polymorphonuclear leukocyte / lipopolysaccharide / milk / blood Vet. Res. 32 (2001) [131][132][133][134][135][136][137][138][139][140][141][142][143][144] 131

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Section: Discussionmentioning

confidence: 99%

Local and systemic effects of endotoxin mastitis on the chemiluminescence of milk and blood neutrophils in dairy cows

et al. 2001

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“…In order to gain insight into the molecular mechanism by which 17β-estradiol affects PMN migration, the influence of 17β-estradiol on the expression of molecules important in PMN migration [18,26,32], was evaluated. The participation of the β 2 -integrin subunits CD11b and CD18 in the migratory process is well established for human as well as for bovine PMN (reviewed in [32,39]). The presence and functional role of CD47 is much less documented than that of CD11b and CD18, and not yet reported for bovine PMN.…”

Section: Discussionmentioning

confidence: 99%

“…PMN were pretreated with antibodies to human CD47 or bovine CD18 (positive control) [32] during 30 min at 37 °C before being added to the upper chamber of the in vitro diapedesis model. Inserts were placed upright in 24-well plates.…”

Section: Pmn Diapedesis Assaymentioning

confidence: 99%

“…Adhesion molecules are involved in this process. Among the latter, the β 2 -integrin subunits CD11b and CD18 are of critical importance for migration of bovine PMN through collagen and epithelial cells [32]. Additionally, an integrin associated protein termed CD47 has more recently been shown to play an important role in the migration of human PMN through intestinal epithelial cells and collagen [18].…”

Section: Introductionmentioning

confidence: 99%

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Influence of sex steroids on the viability and CD11b, CD18 and CD47 expression of blood neutrophils from dairy cows in the last month of gestation

et al. 2006

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-In the period around parturition, cows experience an increased susceptibility for the development of Escherichia coli mastitis. This increased susceptibility has been correlated with a decreased functionality of neutrophils. In the current study, it is suggested that the decreased neutrophil functionality may be induced by the extensive alterations in sex steroid levels occurring around parturition. It was first hypothesized that 17β-estradiol and progesterone influence the viability, apoptosis and necrosis of blood neutrophils from cows in their last month of gestation. Subsequently, it was hypothesized that 17β-estradiol modulates the expression of CD11b, CD18 or CD47 thereby explaining its influence on the migration of bovine neutrophils. Neither 17β-estradiol nor progesterone significantly influenced viability, apoptosis or necrosis in spontaneous apoptosis conditions. However, when apoptosis was induced with TNF-α and gliotoxin, progesterone exerted a survival effect (P < 0.05). In addition, 17β-estradiol treatment of bovine blood neutrophils significantly decreased the expression of CD47 (P < 0.05) but not of CD11b or CD18. It can be concluded that 17β-estradiol and progesterone do not affect spontaneous apoptosis of bovine blood neutrophils while a survival effect was observed for progesterone on induced neutrophils apoptosis. Moreover, our results concerning the influence of 17β-estradiol on the CD11b, CD18 and CD47 expression extend previous demonstrations of the suppressive effect of 17β-estradiol on neutrophils migration and indicate that the altered expression of CD47 may contribute to this phenomenon. sex steroid / β 2 -integrin / CD47 / viability / polymorphonuclear neutrophil leukocyte

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“…Blood PMN are recruited across inflammation. There is, however, sparse information on the underlying mechanisms governing PMN trafficking through the mammary ECM and alveolar epithelium into the milk spaces [16]. The ECM and epithelial basement membrane of the mammary gland and other tissues consist of a complex matrix of mucopolysaccharides produced by various interstitial cells and the alveolar epithelium [7,17].…”

Section: Introductionmentioning

confidence: 99%

CD44 is highly expressed on milk neutrophils in bovine mastitis and plays a role in their adhesion to matrix and mammary epithelium

Gonen¹,

Nedvetzki²,

Naor³

et al. 2008

Vet. Res.

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-Mastitis, inflammation of the mammary gland, is a common and economically important disease in dairy animals. Mammary pathogenic organisms, such as Escherichia coli, invade the teat canal, milk ducts, and mammary alveolar space, replicate in mammary secretions, and elicit a local inflammatory response characterized by massive recruitment of blood polymorphonuclear neutrophil leukocytes (PMN) into the alveoli and milk ducts. CD44 is a trans-membrane glycoprotein previously shown to play a role in mediation and control of blood PMN recruitment in response to inflammatory signals. Here we show, for the first time, increased expression of CD44 on recruited milk PMN in bovine mastitis and the expression of a CD44 variant, CD44v10, on these PMN. Furthermore, we demonstrate that CD44 mediates specific adhesion of bovine blood PMN to hyaluronic acid and mammary epithelial cells. Our results suggest that in mastitis CD44 plays a role in recruiting blood PMN into the mammary glands, the exact nature of this role needs to be elucidated. mastitis / CD44 / neutrophils

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Adhesion receptor CD11b/CD18 contributes to neutrophil diapedesis across the bovine blood–milk barrier

Cited by 32 publications

References 33 publications

Local and systemic effects of endotoxin mastitis on the chemiluminescence of milk and blood neutrophils in dairy cows

Local and systemic effects of endotoxin mastitis on the chemiluminescence of milk and blood neutrophils in dairy cows

Influence of sex steroids on the viability and CD11b, CD18 and CD47 expression of blood neutrophils from dairy cows in the last month of gestation

CD44 is highly expressed on milk neutrophils in bovine mastitis and plays a role in their adhesion to matrix and mammary epithelium

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