Adhesion profile and differentiation capacity of human adipose tissue derived mesenchymal stem cells grown on metal ion (Zn, Ag and Cu) doped hydroxyapatite nano-coated surfaces

Bostancıoğlu, Rakibe Beklem; Gürbüz, Mevlüt; Akyurekli, Ayse Gul; Doğan, Aydın; Koparal, Ali Savaş; Koparal, Ayşe Tansu

doi:10.1016/j.colsurfb.2017.04.015

Cited by 31 publications

(15 citation statements)

References 38 publications

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“…On the other hand, cell adhesion depends on the surface roughness. 59 Webster et al reported that nano-grained ceramics with surface structures 100 nm can improve the bioactivity of Ti implants and enhance osteoblast adhesion. 60,61 In this aspect, nanoscaled modification by FAgHA is an alternative route to improve the osseointegration of Ti-based orthopedic implants.…”

Section: Cell Morphologymentioning

confidence: 99%

“…The growth of the cells may be retarded by the roughness caused by the HA particles in microstructure composites, although nanocomposites themselves promote cell proliferation. 59 The FAgHA nanocomposite can be of great potential applications in the field of bone tissue engineering for the growth and cell proliferation of osteoblasts and bone formation. However, the possibility of toxicity due to the accumulation of Ag ions in body fluids raises the concern for the use of Ag in the FHA.…”

Section: Alp Activity Testmentioning

confidence: 99%

“…Their small amounts can even have positive effects on cells and can improve cytocompatibility. 59 International Journal of Nanomedicine downloaded from https://www.dovepress.com/ by 44.224.250.200 on 17-Oct-2020 For personal use only.…”

Section: Alp Activity Testmentioning

confidence: 99%

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Nanostructured Ag+-substituted fluorhydroxyapatite-TiO2 coatings for enhanced bactericidal effects and osteoinductivity of Ti for biomedical applications

Huang

Song

Chang

et al. 2018

IJN

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BackgroundPoor mechanical properties, undesirable fast dissolution rate, and lack of antibacterial activity limit the application of hydroxyapatite (HA) as an implant coating material. To overcome these limitations, a hybrid coating of Ag+-substituted fluorhydroxyapatite and titania nanotube (TNT) was prepared.MethodsThe incorporation of silver into the HA-TiO2 hybrid coating improves its antimicrobial properties. The addition of F as a second binary element increases the structural stability of the coating. The TNT/F-and-Ag-substituted HA (FAgHA) bilayer coating on the Ti substrate was confirmed by X-ray diffraction, scanning electron microscope, energy-dispersive X-ray spectroscopy (EDS), and X-ray photoelectron spectroscopy (XPS).ResultsThe results indicate that the FAgHA/TNT nanocomposite coating has a dense and uniform morphology with a nano-rod-like structure. The solubility measurement result shows that the substitution of F− ions into the AgHA structure has a positive effect on the dissolution resistance of HA. The adhesion strength of FAgHA/TNT has significantly increased because of the interlocking of the roughened surface with nano-rod-like particles that entered into the voids of the TiO2 nanotubes. Compared with that of the bare Ti, the corrosion current density of FAgHA/TNT-coated Ti substrate decreased from 3.71 to 0.18 μA, and its corrosion resistance increased by almost two orders of magnitude. Moreover, despite pure HA, the FAgHA killed all viable Staphylococcus aureus after 24 hours of incubation. Although the fabricated FAgHA/TNT coating is hydrophobic, it induced deposition of the typical spherical apatite when immersed in a simulated body fluid (SBF); the osteoblasts spread very well on the surface of the coating. In addition, in vitro cell culture tests demonstrated cell viability and alkaline phosphatase (ALP) similar to pure HA, which indicated good cytocompatibility. Interestingly, compared with bare Ti, FAgHA/TNT-coated Ti surface was innocent for cell vitality and even more beneficial for cell osteogenesis in vitro.ConclusionEnhancing the osseointegration and preventing infection in implants, the FAgHA/TNT-coated Ti makes implants more successful.

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Section: Cell Morphologymentioning

confidence: 99%

Section: Alp Activity Testmentioning

confidence: 99%

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Nanostructured Ag+-substituted fluorhydroxyapatite-TiO2 coatings for enhanced bactericidal effects and osteoinductivity of Ti for biomedical applications

Huang

Song

Chang

et al. 2018

IJN

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“…The isolation process produces the stromal vascular fraction (SVF), a heterogenous cell population containing different cell types, including ASCs, endothelial precursor cells (EPCs), endothelial cells (ECs), lymphocytes, macrophages, smooth muscle cells, pericytes, and pre-adipocytes [Bora and Majumdar, 2017]. Culturing the SVF on a coatingfree plastic surface leads to ASC adherence and proliferation, a key characteristic of ASCs/MSCs, while culture on specific surfaces can lead to unwanted effects on e.g., the proliferation and initiation of differentiation of ASCs [Van Dijk et al, 2008;Somaiah et al, 2015;Bostancioglu et al, 2017;Gao et al, 2019]. Positive staining for CD73, CD90, and CD105 as well as negative staining for CD31 and CD45 are required to phenotypically define ASCs [Bourin et al, 2014].…”

Section: Introductionmentioning

confidence: 99%

Adipose-Derived Stromal/Stem Cell Culture: Effects of Different Concentrations of Human Platelet Lysate in Media

Rivera‐Ballesteros

Brooks

Haastrup

et al. 2020

Cells Tissues Organs

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Adipose-derived stromal/stem cells (ASCs) are being tested as a possible treatment for a wide range of diseases to exploit the immunomodulatory and regenerative potential demonstrated in vitro. Pooled human platelet lysate (pHPL) has replaced fetal bovine serum (FBS) as the preferred growth supplement because of its xeno-free origin and improved cell proliferation. Much has been done toward reducing the concentration of pHPL required when expanding ASCs. However, little is known on how increasing the concentration of pHPL affects ASC potency, which could lead to changes with possible beneficial applications. This study investigated the effect of 5, 10, or 20% pHPL in culture media on ASC proliferation and phenotypic marker expression, including chemokine receptors CXCR2, CXCR3, CXCR4, and VLA-4. Adipogenic and osteogenic properties, as well as immunosuppressive properties, including the ability to induce indoleamine-pyrrole 2,3-dioxygenase 1 (IDO1) and suppress T cell proliferation, were also examined. We observed a significant increase in cell yield (approximately 2-fold) and a corresponding reduction in population doubling time and cell volume when doubling the concentration of pHPL in the growth media. ASCs maintained expression of phenotypic surface markers CD73, CD90, and CD105 and were negative for CD45 and CD31. The ability to induce IDO1 and suppress T cell proliferation was observed as well. Adipogenesis and osteogenesis, however, seem to be increased at higher concentrations of pHPL (20% > 10% > 5%), while expression of chemokine receptors CXCR2 and CXCR3 was lower. In conclusion, increasing the pHPL concentration to 20% could be used to optimize culture conditions when producing cells for clinical treatments and may even be used to enhance beneficial ASC properties depending on the desired therapeutic effect.

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“…Thus, aiming to firstly improve the cell adhesion, three different microcarrier coatings were tested in spinner flasks: 10% (v/v) and 20% (v/v) of AB HS and fibronectin. The latter was used as a control due to its wide use as an adhesion protein for MSC cultivation (Kang et al, 2014;Bostancioglu et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Successful Use of Human AB Serum to Support the Expansion of Adipose Tissue-Derived Mesenchymal Stem/Stromal Cell in a Microcarrier-Based Platform

Moreira

Mizukami

Souza

et al. 2020

Front. Bioeng. Biotechnol.

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Mesenchymal stem/stromal cells (MSC) are promising candidates for cell-based therapies and for the promotion of tissue repair, hence the increase of clinical trials in a worldwide scale. In particular, adipose tissue-derived stem/stromal cells (AT MSC) present easy accessibility and a rather straightforward process of isolation, providing a clear advantage over other sources. The high demand of cell doses (millions of cells/kg), needed for infusion in clinical settings, requires a scalable and efficient manufacturing of AT MSC under xenogeneic(xeno)-free culture conditions. Here we describe the successful use of human AB serum [10%(v/v)] as a culture supplement, as well as coating substrate for the expansion of these cells in microcarriers using (i) a spinner flask and (ii) a 500-mL mini-bioreactor (Applikon TM Biotechnology). Cells were characterized by immunophenotype and multilineage differentiation potential. Upon an initial cell adhesion in the spinner flask of 35 ± 2.5%, culture reached a maximal cell density of 2.6 ± 0.1 × 10 5 at day 7, obtaining a 15 ± 1-fold increase. The implementation of the culture in the 500-mL mini-bioreactor presented an initial cell adhesion of 22 ± 5%, but it reached maximal cell density of 2.7 ± 0.4 × 10 5 at day 7, obtaining a 27 ± 8-fold increase. Importantly, in both stirred systems, cells retained their immunophenotype and multilineage differentiation potential (osteo-, chondro-and adipogenic lineages). Overall, the scalability of this microcarrier-based system presented herein is of major importance for the purpose of achieving clinically meaningful cell numbers.

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Adhesion profile and differentiation capacity of human adipose tissue derived mesenchymal stem cells grown on metal ion (Zn, Ag and Cu) doped hydroxyapatite nano-coated surfaces

Cited by 31 publications

References 38 publications

Nanostructured Ag<sup>+</sup>-substituted fluorhydroxyapatite-TiO<sub>2</sub> coatings for enhanced bactericidal effects and osteoinductivity of Ti for biomedical applications

Nanostructured Ag<sup>+</sup>-substituted fluorhydroxyapatite-TiO<sub>2</sub> coatings for enhanced bactericidal effects and osteoinductivity of Ti for biomedical applications

Adipose-Derived Stromal/Stem Cell Culture: Effects of Different Concentrations of Human Platelet Lysate in Media

Successful Use of Human AB Serum to Support the Expansion of Adipose Tissue-Derived Mesenchymal Stem/Stromal Cell in a Microcarrier-Based Platform

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