Adhesion of the genome-sequenced Lactococcus lactis subsp. cremoris IBB477 strain is mediated by specific molecular determinants

Abstract

Understanding the nature of mucus-microbe interactions will provide important information that can help to elucidate the mechanisms underlying probiotic adhesion. This study focused on the adhesive properties of the Lactococcus lactis subsp. cremoris IBB477 strain, previously shown to persist in the gastrointestinal tract of germ-free rats. The shear flow-induced detachment of L. lactis cells was investigated under laminar flow conditions. Such a dynamic approach demonstrated increased adhesion to bare and mucin-coated polystyrene for IBB477, compared to that observed for the MG1820 control strain. To identify potential genetic determinants giving adhesive properties to IBB477, the improved high-quality draft genome sequence comprising chromosome and five plasmids was obtained and analysed. The number of putative adhesion proteins was determined on the basis of surface/extracellular localisation and/or the presence of adhesion domains. To identify proteins essential for the IBB477 specific adhesion property, nine deletion mutants in chromosomal genes have been constructed and analysed using adhesion tests on bare polystyrene as well as mucin-, fibronectin- or collagen IV-coated polystyrene plates in comparison to the wild-type strain. These experiments demonstrated that gene AJ89_07570 encoding a protein containing DUF285, MucBP and four Big_3 domains is involved in adhesion to bare and mucin-coated polystyrene. To summarise, in the present work, we characterised the adhesion of IBB477 under laminar flow conditions; identified the putative adherence factors present in IBB477, which is the first L. lactis strain exhibiting adhesive and mucoadhesive properties to be sequenced and demonstrated that one of the proteins containing adhesion domains contributes to adhesion.Electronic supplementary materialThe online version of this article (doi:10.1007/s00253-016-7813-0) contains supplementary material, which is available to authorized users.

Contribution of plasmid-encoded peptidase S8 (PrtP) to adhesion and transit in the gut of Lactococcus lactis IBB477 strain

“…With regard to our previous results (Radziwill-Bienkowska et al 2016), we conclude that both chromosomal and plasmid-encoded molecular factors contribute to adhesive properties of L. lactis IBB477.…”

In vitro characteristics of Lactobacillus spp. strains isolated from the chicken digestive tract and their role in the inhibition of Campylobacter colonization

“…It is postulated that highly adhesive probiotic bacteria have the greatest beneficial effects on host health and they should, at least transiently, colonize the host gut (Bermudez‐Brito et al, ). The adhesion microtiter plate assay is a well‐known and simple method for a preliminary assessment of strain adhesion ability (Radziwill‐Bienkowska et al., ), which, as we observed in our previous studies, can correlate with its adhesiveness to biotic surfaces (Aleksandrzak‐Piekarczyk et al., ; Radziwill‐Bienkowska et al., , ). In this study, we checked the adhesive properties of 64 Lactobacillus strains isolated from chicken gastrointestinal tracts and compared them with highly adhesive or nonadhesive L. rhamnosus strains (GG or LOCK 0908, respectively).…”

Impact of Prebiotics and Synbiotics Administered in ovo on the Immune Response against Experimental Antigens in Chicken Broilers

“…The lactobacilli bacteria used in the experiment of Brisbin et al [10] are members of the chicken intestinal microbiota. In the present study, the bacterial strains were given only once, in ovo, in the air chamber at E12, and were not identified as a natural component of the chicken microbiome [23,45]. The presence of a low agglutination titer on D7 (in individual chickens from each group before immunization), as observed in our study (Figure 3), indicates the occurrence of a natural antibody that cross reacts with SRBC [46].…”