Adhesion of macrophages on collagen irradiated with ultraviolet light

Watanabe, Kazuo; Koyama, Yoh-ichi

doi:10.1163/156856299x00414

Cited by 6 publications

(5 citation statements)

References 35 publications

(33 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1994), and a variety of biopolymers (CS, HA, alginate, polyethylene glycol, fibrin, fibronectin) (Lee et al 2001a; Stalling et al 2009; Erickson et al 2012) which can be entrapped or crosslinked after incorporating functional groups such as methacrylate, thiolate or tyramine have been shown to be efficacious and biocompatible with controlled cytotoxicity levels when used in cell culture, animal and human studies (Akens et al, 2002; von Rechenberg et al, 2004; Bryant et al, 2000; Sharma et al, 2013). The properties of crosslinked collagen have been extensively investigated and benefits include enhanced mechanical properties (Wollensak et al, 2003b; Weadock et al, 1995; Koide and Daito, 1997), reduced susceptibility to enzymatic degradation (Spoerl et al, 2004; Stanescu and Stanescu, 1988; Lee et al, 2001b), and reduced adhesion of macrophages (Van Luyn et al, 1998; Bailey, 2000; Watanabe and Koyama, 1999). …”

Section: Discussionmentioning

confidence: 99%

Resurfacing damaged articular cartilage to restore compressive properties

Grenier

Donnelly

Gittens

et al. 2015

Journal of Biomechanics

View full text Add to dashboard Cite

Surface damage to articular cartilage is recognized as the initial underlying process causing the loss of mechanical function in early-stage osteoarthritis. In this study, we developed structure-modifying treatments to potentially prevent, stabilize or reverse the loss in mechanical function. Various polymers (chondroitin sulfate, carboxymethylcellulose, sodium hyaluronate) and photoinitiators (riboflavin, irgacure 2959) were applied to the surface of collagenase-degraded cartilage and crosslinked in situ using UV light irradiation. While matrix permeability and deformation significantly increased following collagenase-induced degradation of the superficial zone, resurfacing using tyramine-substituted sodium hyaluronate and riboflavin decreased both values to a level comparable to that of intact cartilage. Repetitive loading of resurfaced cartilage showed minimal variation in the mechanical response over a 7 day period. Cartilage resurfaced using a low concentration of riboflavin had viable cells in all zones while a higher concentration resulted in a thin layer of cell death in the uppermost superficial zone. Our approach to repair surface damage initiates a new therapeutic advance in the treatment of injured articular cartilage with potential benefits that include enhanced mechanical properties, reduced susceptibility to enzymatic degradation and reduced adhesion of macrophages.

show abstract

Section: Discussionmentioning

confidence: 99%

Resurfacing damaged articular cartilage to restore compressive properties

Grenier

Donnelly

Gittens

et al. 2015

Journal of Biomechanics

View full text Add to dashboard Cite

show abstract

“…Various methods for cross-linking the collagen molecule or the gelatin molecule have been reported: dehydrothermal cross-linking [10], glutaraldehyde treatment [3,11], carbodiimide treatment [3], UV irradiation [7,12] and using a photo-curable cross-linker [13].…”

Section: Introductionmentioning

confidence: 99%

A novel collagen hydrogel cross-linked by gamma-ray irradiation in acidic pH conditions

Inoue

Bessho²,

Furuta³

et al. 2006

Journal of Biomaterials Science, Polymer Edition

View full text Add to dashboard Cite

We made a new type of collagen gel by gamma-ray irradiation of an acidic solution of type-I collagen, and performed comparative studies on a conventional gel and the new type of gel. The neutral gel, a conventional 0.3% (w/v) collagen gel, was formed at neutral pH and then irradiated by gamma-rays. The acidic gel, a 0.3% (w/v) collagen gel, was formed directly from the acidic solution of collagen by y-ray irradiation. Both types of gel were prepared, swollen in water and then dried for the measurement of specific water content. The neutral gel showed a relatively high specific water content and shrunk moderately, depending on the dose, while the acidic gel showed lower specific water content and shrunk clearly by y-ray irradiation. A three-dimensional tangled network of microfibrils was clearly observed in the neutral gels by scanning electron microscopy, but not in the acidic gels. From these results, we concluded that the acidic gel was quite different from a conventional collagen gel. Sodium dodecylsulfate-polyacrylamide gel electrophoresis showed that the alpha1 subunit and alpha2 subunit of the collagen molecule were cross-linked. The triple-helical structure of collagen was only partially perturbed, but not denatured completely, because the circular dichroism spectrum of the collagen solution irradiated at 1.3 kGy was similar to that of native collagen solution. Amino-acid analysis revealed that tyrosine, phenylalanine and histidine decreased by irradiation in the neutral gel. In the case of the acidic gel, these three amino acids and methionine decreased. We considered that these amino acids were cross-linking points between the collagen subunits during the gamma-ray irradiation.

show abstract

“…The results show that the CCRMs treated with Co-60 (2 kGy) and EB (2 kGy) can activate M1 macrophages. Overall, the CCRMs treated with UV can downregulate the expression of pro-inflammatory-related genes and upregulate the expression of anti-inflammatory genes, which may be related to the crosslinking of amino acid residues in collagen by ultraviolet light …”

Section: Results and Discussionmentioning

confidence: 99%

“…Interestingly, it is found from Figure 4 45 and upregulate the expression of antiinflammatory genes, which may be related to the crosslinking of amino acid residues in collagen by ultraviolet light. 46 2.5. ELISA.…”

Section: Expression Of Genes Related To Macrophagementioning

confidence: 99%

Effects of Different Radiation Sources on the Performance of Collagen-Based Corneal Repair Materials and Macrophage Polarization

et al. 2022

View full text Add to dashboard Cite

Owing to the lack of donor corneas, there is an urgent need for suitable corneal substitutes. As the main component of the corneal stroma, collagen has great advantages as a corneal repair material. If there are microorganisms such as bacteria in the corneal repair material, it may induce postoperative infection, causing the failure of corneal transplantation. Therefore, irradiation, as a common sterilization method, is often used to control the microorganisms in the material. However, it has not been reported which type of radiation source and what doses can sterilize more effectively without affecting the properties of collagen-based corneal repair materials (CCRMs) and have a positive impact on macrophage polarization. In this study, three different radiation sources of ultraviolet, cobalt-60, and electron beam at four different doses of 2, 5, 8, and 10 kGy were used to irradiate CCRMs. The swelling, stretching, transmittance, and degradation of the irradiated CCRMs were characterized, and the proliferation of human corneal epithelial cells on the irradiated CCRMs was characterized using the CCK8 kit. The results showed that low dose (<5 kGy) of radiation had little effect on the performance of CCRMs. Three irradiation methods with less influence were selected for the further study on RAW264.7 macrophage polarization. The results indicated that CCRMs treated with UV could downregulate the expression of pro-inflammatory related genes and upregulate the expression of anti-inflammatory genes in macrophages, which indicated that UV irradiation is a beneficial process for the preparation of CCRMs.

show abstract

Adhesion of macrophages on collagen irradiated with ultraviolet light

Cited by 6 publications

References 35 publications

Resurfacing damaged articular cartilage to restore compressive properties

Resurfacing damaged articular cartilage to restore compressive properties

A novel collagen hydrogel cross-linked by gamma-ray irradiation in acidic pH conditions

Effects of Different Radiation Sources on the Performance of Collagen-Based Corneal Repair Materials and Macrophage Polarization

Contact Info

Product

Resources

About