Adhesion of Latex Spheres to Giant Phospholipid Vesicles: Statics and Dynamics

Dietrich, Christian; Angelova, Maria; Pouligny, B.

doi:10.1051/jp2:1997208

Cited by 120 publications

(153 citation statements)

References 11 publications

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“…Trapping can be simply achieved using not one, but a couple of counterpropagating beams. The concept, which has been explored in the 1970s, [2][3][4]17 and later used in a few applications, [5][6][7][8] is illustrated in Figs. 1͑b͒ and 1͑c͒.…”

Section: A Basicsmentioning

confidence: 99%

“…[5][6][7][8] Much more widespread is the so-called "optical tweezer" geometry, 9 based on a single tightly focused beam. In comparison to the two-beam concept, the single-beam trap has the twofold advantage of simplicity and size independence; i.e., particles with sizes ranging from less than 1 m up to about 20 m can be trapped with the same setup, without any adjustment except that of the beam power.…”

Section: Introductionmentioning

confidence: 99%

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Implementing both short- and long-working-distance optical trappings into a commercial microscope

Kraikivski

Pouligny

Dimova

2006

Review of Scientific Instruments

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Optical tweezers are now a widespread tool based on three-dimensional trapping by a tightly focused single laser beam. This configuration only works with large numerical aperture and short-working-distance (SWD) objectives, restricting optical manipulation to the high magnification end of the microscope nosepiece. Certain applications of optical trapping demand long-working distances (LWDs) at moderate magnification, imposing a more complex two-beam trapping configuration. In this article, we describe a complete setup that incorporates both SWD and LWD optical trapping functionalities into a single Axiovert 200M Zeiss microscope. We evaluate the performance of the setup in both trapping modes with latex particles, either fluorescent or not, of different sizes, in the 1–20μm range. We provide practical information allowing for optimal configuration of the two-beam geometry, in relation with longitudinal and lateral stabilities of the trap.

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Section: A Basicsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Implementing both short- and long-working-distance optical trappings into a commercial microscope

Kraikivski

Pouligny

Dimova

2006

Review of Scientific Instruments

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“…A typical situation is shown in Fig. 1; here a Recently, it was shown by Angelova et al [1] is far from 0 or 180°, which means that the particle and Dietrich et al [2] that micrometre-sized intercepts a definite portion of the vesicle contour. spheres of polystyrene or glass could be attached If the membrane is in the fluid state (this is so, to lipid bilayers without any special surface treatfor instance, with egg lecithin at room temperment.…”

Section: Introductionmentioning

confidence: 94%

“…2. In such an experiment, the particle plays steps [2], but after equilibrium has been achieved, the role of a macroscopic mechanical probe, which a takes on a constant value. This value is not may be used to feel the friction opposed by the universal, as it depends strongly on the initial membrane to its motion.…”

Section: Introductionmentioning

confidence: 99%

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Motion of a massive particle attached to a spherical interface: statistical properties of the particle path

Velikov¹,

Danov²,

Angelova³

et al. 1999

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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We have studied the motion of a Brownian particle on a spherical interface under gravity, with the aim of setting up a protocol to measure the friction (f) felt by such a particle in experimental conditions. Our analysis is based on the Schmoluchowski equation for particle motion. Essentially we derive a practical criterion to find f from the average particle path. Our statements are illustrated by a few experimental and numerical examples. Numerical paths are obtained by computer simulation and experimental paths are those of micrometre-sized latex or glass particles attached to spherical giant lipid (SOPC ) vesicles. From experimental values of f, we estimate the surface shear viscosity of SOPC bilayers to be in the range 3-8×10−6 Poise.

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Giant Vesicles: Micromanipulation of Membrane Bilayers

Menger¹,

Keiper

1998

Adv. Mater.

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Lipid membranes attract throngs of scientists with diverse goals, ranging from the discovery of new materials to the determination of cell-fusion mechanisms. Conventionally, one of two modes of study is selected: a) spectroscopic and kinetic analysis of submicroscopic lipid vesicles or b) characterization of lipid films. These methods have yielded substantial information on the structure and cooperative nature of lipid assemblies. Yet neither approach is fully satisfying. Interpretations are often based on inference rather than on direct observation. In the case of vesicular membranes, data are ªaveragesº, i.e., taken over large populations rather than on an individual entity. With both submicroscopic vesicles and deposited films it is difficult to assess important morphological changes, e.g., the healing of damaged membranes or endocytosis and other membrane distortions.

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Adhesion of Latex Spheres to Giant Phospholipid Vesicles: Statics and Dynamics

Abstract: We studied the sequence of phenomena which occur when a solid microsphere is brought in contact with an isolated giant lipid vesicle. We used Latex beads, a few microns in diameter, which were manipulated individually by means of a long-working-distance optical trap.

Cited by 120 publications

References 11 publications

Implementing both short- and long-working-distance optical trappings into a commercial microscope

Implementing both short- and long-working-distance optical trappings into a commercial microscope

Motion of a massive particle attached to a spherical interface: statistical properties of the particle path

Giant Vesicles: Micromanipulation of Membrane Bilayers

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