Adhesion of L929 mouse ribroblast cells on poly(styrene)/poly(methyl methacrylate) films

Abstract

Filmes de poli(estireno) (PS), poli(metacrilato de metila) (PMMA), blenda de PS/PMMA (1:1) e copolímero PS-b-PMMA foram preparados e avaliados quanto à adesão celular usando fibroblastos de camundongos L929. Embora todos os filmes poliméricos tenham se mostrado bons substratos para o crescimento e proliferação celular, estes processos foram levemente favorecidos na blenda PS:PMMA. O número e a morfologia foram idênticos para cultura de células nos filmes e na lamínula de vidro ou na placa de plástico. A característica química dos filmes poliméricos é adequada para suportar o ataque e proliferação das células, sugerindo que esses filmes são bons candidatos para usos biomédicos.Films of pure poly(styrene) (PS), pure poly(methyl methacrylate) (PMMA), a 1:1 PS/PMMA blend and a PS-b-PMMA copolymer, were prepared and tested for cell adhesion using L929 mouse fibroblasts. All polymer films were found to be good substrates for cell adhesion and proliferation, and both processes were slightly favored on films of the 1:1 PS/PMMA blend. The same results were obtained in terms of cell number and morphology for cells cultured on films, glass coverslips or plastic plates. The chemical characteristics of polymer films make them suitable supports for cell attachment and proliferation, indicating that these films are good candidates for biomedical uses.

Polymer films with surfaces unmodified and modified by non-thermal plasma as new substrates for cell adhesion

“…2(A and B) are in agreement with data published in the literature [26], and are promising in terms of the potential use of the PS/PMMA blend in biomedical applications. Some factors such as the surface wettability of polymer films can influence the adhesion and proliferation of cells, since the first layer of the cell that touches the surface is comprised of water [10,11].…”

“…in the blend) [26]. Considering this result, the aim of the study reported herein was to verify the advantages of using this film as a biomaterial.…”

“…4 shows the AFM images of the unmodified and modified PS, 1:1 PS/PMMA and PMMA films. As can be observed, the film originating from the blend (1:1 PS/PMMA) has low and high regions caused by the phase separation of its components [26]. Hence, a preliminary study with AFM images was necessary.…”

“…Although all films showed chemical properties able to support cell adherence and proliferation, these processes were slightly favored in the blend. The authors concluded that the use of the 1:1 blend in future experiments would be advantageous since this material has an additional benefit which is its cost-effectiveness [26].…”

Super-resolved 3-D imaging of live cells’ organelles from bright-field photon transmission micrographs

“…Similar results have been depicted in scanning microscopy images and described in literature. [23][24][25][26] For the microscopy experiments, the dish bottoms were not treated.…”