Adhesion of L1210 cells to sulfonated styrene copolymer surfaces in the absence of serum

Kowalczyńska, Hanna M.

doi:10.1016/0927-7765(94)01163-y

Cited by 16 publications

(60 citation statements)

References 32 publications

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“…These interactions were related to five parameters such as type and percent distribution of the chemical groups on the surface [1], surface roughness [1][2][3][4][5], surface wettability [6][7][8], surface free energy (SFE) of the substrate [6,9], and size and shape of the cell used for testing [10,11]. The distance between surface patterns and focal adhesion clusters on the cell membrane is also an important parameter for the cell to form a strong adhesion on a substrate [1][2][3]12,13].…”

Section: Introductionmentioning

confidence: 99%

Selective adsorption of L1210 leukemia cells/human leukocytes on micropatterned surfaces prepared from polystyrene/polypropylene-polyethylene blends

Gengec¹,

Gulsuner²,

Erbil³

et al. 2014

Colloids and Surfaces B: Biointerfaces

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a b s t r a c tThe objective of this study is to prepare polymeric surfaces which will adsorb L1210 leukemia cells selectively more than that of healthy human leukocytes in order to develop new treatment options for people with leukemia. Chemically heterogeneous and micropatterned surfaces were formed on round glass slides by dip coating with accompanying phase-separation process where only commercial polymers were used. Surface properties were determined by using optical microscopy, 3D profilometry, SEM and measuring contact angles. Polymer, solvent/nonsolvent types, blend composition and temperature were found to be effective in controlling the dimensions of surface microislands. MTT tests were applied for cell viability performance of these surfaces. Polystyrene/polyethylene-polypropylene blend surfaces were found to show considerable positive selectivity to L1210 leukemia cells where L1210/healthy leukocytes adsorption ratio approached to 9-fold in vitro. Effects of wettability, surface free energy, microisland size geometry on the adsorption performances of L1210/leukocytes pairs are discussed.

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Section: Introductionmentioning

confidence: 99%

Selective adsorption of L1210 leukemia cells/human leukocytes on micropatterned surfaces prepared from polystyrene/polypropylene-polyethylene blends

Gengec¹,

Gulsuner²,

Erbil³

et al. 2014

Colloids and Surfaces B: Biointerfaces

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“…However, these results do not To the best of our knowledge, the present work is the first attempt to describe the interaction of PC-3 cells with biologically active human plasma fibronectin adsorbed on strongly polar polystyrene surfaces of a defined chemical composition and topography [14,22].…”

Section: Discussionmentioning

confidence: 73%

“…The polystyrene solution after dilution with toluene to 5% concentration was deposited onto specially cleaned glass Sulfonation of polystyrene surfaces. The surfaces of polystyrene films were sulfonated according to the original method previously described [22,37]. The sulfonation reaction was carried out in a water-free atmosphere at 18°C with sulfur trioxide, a highly active sulfonating agent , at the gas/ /polymer interface.…”

Section: Methodsmentioning

confidence: 99%

“…The chamber bottom consisted of a cover slip coated with a polymer film. In the experiments with the shortest cell-substratum interaction time (t int = 30 and 60 s), another type of chamber (120 μm height) was used, as previously described in detail [41]. The cell concentration was 0.5 × 10 6 /ml.…”

Section: Fibronectin Isolationmentioning

confidence: 99%

“…Fibronectin adsorbed on strongly hydrophilic polystyrene surfaces, in contrast to the adsorption on hydrophobic surfaces, is characterized by extended, biologically active conformation with the unfolded cellbinding domain (CCBD), which stimulates not only the early phase of adhesion, but also the cell spreading process [13,14,20,21]. The polystyrene surfaces used in the present work have been modified according to the original method developed by Kowalczyń-ska and Kamiński [22], which unlike other methods of surface modification [16,[23][24][25], is mild and highly efficacious, making it possible to eliminate the harmful hydrolyzing effect of sulfuric acid.…”

Section: Introductionmentioning

confidence: 99%

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Interaction of PC-3 cells with fibronectin adsorbed on sulfonated polystyrene surfaces

Stachurska

Kowalczyńska²

2012

Folia Histochem Cytobiol.

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Abstract:The ability of cancer cells to invade neighboring tissues is crucial for cell dissemination and tumor metastasis. It is generally assumed that cell adhesion to extracellular matrix proteins is an important stage of cancer progression. Hence, adhesion of cancer cells under in vitro conditions to proteins adsorbed on a substratum surface has been studied to provide a better understanding of cell-protein interaction mechanisms. A protein, adsorbed in an appropriate conformation on a substratum surface, creates a biologically active layer that regulates such cell functions as adhesion, spreading, proliferation and migration. In our study, we examined the interaction of PC-3 cells under in vitro conditions with fibronectin adsorbed on sulfonated polystyrene surfaces of a defined chemical composition and topography. We investigated cell adhesion to fibronectin and cell spreading. Using automatic, sequential microscopic image registration, we are the first to present observations of the dynamics of PC-3 cell spreading and the cell shape during this process. Our results show that cell adhesion and the shape of spreading cells strongly depend on the time interaction with fibronectin. The analysis of images of cytoskeletal protein distribution in the cell region near the cell-substratum interface revealed that induction of a signal cascade took place, which led to the reorganization of the cytoskeletal proteins and the activation of focal adhesion kinase (FAK).

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Signal transduction and cytoskeletal reorganization are required for cell detachment from cell culture surfaces grafted with a temperature-responsive polymer

Yamato

Okuhara

Karikusa

et al. 1999

J. Biomed. Mater. Res.

134

105

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We have developed a new cell culture substrate grafted with a temperature-responsive polymer, poly(N-isopropylacrylamide) (PIPAAm) using an electron beam irradiation method. These surfaces are hydrophobic in culture at 37 degrees C due to the hydration/dehydration changes intrinsic to PIPAAm at 32 degrees C, and they become highly hydrophilic below 32 degrees C. At 37 degrees C grafted and ungrafted surfaces showed no difference with regard to attachment, spreading, growth, confluent cell density, and morphology of bovine aortic endothelial cells. Stress fibers, peripheral bands, and focal contacts were established in similar ways. After the medium temperature was decreased to 20 degrees C, spread cells lost their flattened morphology, acquiring a rounded cell appearance similar to that of cells immediately after plating. After mild agitation cells floated free from the dish surface without trypsin treatment. Neither cell morphological changes nor cell detachment occurred on ungrafted surfaces. An ATP synthesis inhibitor, sodium azide, and a tyrosine kinase inhibitor, genistein, suppressed cell morphological changes and cell detachment while a protein synthesis inhibitor, cycloheximide, slightly enhanced cell detachment. An actin filament stabilizer, phalloidin, and its depolymerizer, cytochalasin D, also inhibited cell detachment. These findings suggest that cell detachment on grafted surfaces is mediated by intracellular signal transduction and reorganization of the cytoskeleton. While trypsinization causes damage to the cell membrane surface and extracellular matrix proteins, this alternative low temperature treatment is exceptionally noninvasive. The temperature-responsive cell culture surface also should prove useful for investigating the molecular machinery involved in cell-surface detachment.

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Adhesion of L1210 cells to sulfonated styrene copolymer surfaces in the absence of serum

Cited by 16 publications

References 32 publications

Selective adsorption of L1210 leukemia cells/human leukocytes on micropatterned surfaces prepared from polystyrene/polypropylene-polyethylene blends

Selective adsorption of L1210 leukemia cells/human leukocytes on micropatterned surfaces prepared from polystyrene/polypropylene-polyethylene blends

Interaction of PC-3 cells with fibronectin adsorbed on sulfonated polystyrene surfaces

Signal transduction and cytoskeletal reorganization are required for cell detachment from cell culture surfaces grafted with a temperature-responsive polymer

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