Adhesion of <i>Legionella pneumophila</i> on glass and plumbing materials commonly used in domestic water systems

Assaidi, Abdelwahid; Ellouali, Mostafa; Latrache, Hassan; Mabrouki, Mustapha; Timinouni, Mohammed; Zahir, Hafida; Tankiouine, Safae; Barguigua, Abouddihaj; Mliji, El Mostafa

doi:10.1080/09603123.2018.1429580

Cited by 17 publications

(10 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several studies have reported that the growth and the proliferation of L. pneumophila are influenced by many factors, such as pipe materials, temperature, stagnation, flow circumstances, corrosion, and pipe roughness (Assaidi et al 2018a(Assaidi et al , 2018b. In our study, the aggravating effect of the pipe materials on colonization was also confirmed.…”

Section: Discussionsupporting

confidence: 82%

Environmental surveillance of Legionella pneumophila in hot water systems of hotels in Morocco

Assaidi

Soummane

Ellouali

et al. 2021

Journal of Water and Health

Self Cite

View full text Add to dashboard Cite

Objective: Environmental monitoring of Legionella in hot water systems of hotels in Morocco was performed during the period from January 2016 to April 2018. A total of 149 water samples from 118 different hotels were analyzed. Methods: A total of 149 water samples from 118 different hotels were analyzed. Possible risk factors were prospectively recorded, and data were analyzed in connection with building and plumbing systems characteristics. Data about building and risk factors were collected through a questionnaire survey. Results: Out of the 149 samples, 77(51.7%) were positive for L. pneumophila. Serological typing of the isolates revealed that 54(70.1%) are L. pneumophila serogroup 2–15 and 23 (29.9%) are L. pneumophila serogroup 1. 56.8% of all building were colonized by L. pneumophila. Counts were over 1,000 CFU/L in 44%. Contamination was strongly correlated with temperature in the circulation, the age of the premise plumbing and the size of the building. Conclusions: The results showed a relevant exposure to L. pneumophila in the community and the identified risk factors can serve as indicators for risk assessment and relevant actions.

show abstract

Section: Discussionsupporting

confidence: 82%

Environmental surveillance of Legionella pneumophila in hot water systems of hotels in Morocco

Assaidi

Soummane

Ellouali

et al. 2021

Journal of Water and Health

Self Cite

View full text Add to dashboard Cite

show abstract

“…According to the literature, the hydrophobicity of a bacterial cell is largely influenced by the residues and structures on the cell surfaces, which can be hydrophilic or hydrophobic [ 10 , 19 , 37 ]. Hence, bacterial hydrophobicity varies among species and strains, even within the same strain, depending on the mode and stage of growth, growth medium composition, and even the analysis technique [ 7 , 9 , 38 , 39 ], potentially explaining the variation of hydrophilicities even within the same species in our work.…”

Section: Discussionmentioning

confidence: 99%

Adhesion Behavior of Escherichia coli Strains on Glass: Role of Cell Surface Qualitative and Quantitative Hydrophobicity in Their Attachment Ability

Elfazazi¹,

Zahir²,

Tankiouine³

et al. 2021

International Journal of Microbiology

Self Cite

View full text Add to dashboard Cite

Microbial adhesion to surfaces is thought to involve physicochemical interactions between the substrate and microbial cells. Understanding the physicochemical aspects involved in the adhesion phenomenon, as a critical step in biofilm formation, is essential to finding ways to prevent their formation and control biocontamination risks. The aim of this study was to investigate the relation between the adhesion behavior of 12 Escherichia coli strains isolated from food and their surface hydrophobicities using qualitative ( θ w ) and quantitative (ΔGiwi) approaches. The surface physicochemical properties of both bacterial cells and glass material were estimated through contact angle measurements. The adhesive behavior of E. coli strains on a glass surface was assessed. The results showed a good logarithmic relation between the percentage of the adhered cells and their surface hydrophobicity with the quantitative approach ΔGiwi; however, qualitative hydrophobicity ( θ w ) appeared to demonstrate no effect regarding adhesion behavior. This work lays the foundation for future studies and opens an important debate on the mechanisms underlying the adhesion behavior of E. coli strains by using the thermodynamic approach (ΔGiwi) as an important model of hydrophobicity that could explain and predict better bacterial adhesion ability.

show abstract

“…Adherence governs the ability of bacteria to colonize different surfaces. For this reason, the limitation of adhesion process is favorable and highly encouraged in the course of the creation of novel biomaterials, both for external use and implanted, as well as the development of anti-infectious therapeutics or production of improved materials for industrial purposes [ 30 , 44 , 102 ]. The capability of AFM to measure adhesion forces between the catilever tip and scanned sample is particularly useful in this manner.…”

Section: Afm-based Investigation As a Novel Approach To Fight Withmentioning

confidence: 99%

“…However, studies have shown that after a long period of time there is no difference between copper and plastic materials [ 127 , 128 ]. In another study, Assaidi et al tested the adhesion changes of Legionella pneumophila serogroup 1 and serogroup 2 to different materials used in water systems [ 102 ].…”

Section: Afm-based Investigation As a Novel Approach To Fight Withmentioning

confidence: 99%

Physics Comes to the Aid of Medicine—Clinically-Relevant Microorganisms through the Eyes of Atomic Force Microscope

et al. 2020

View full text Add to dashboard Cite

Despite the hope that was raised with the implementation of antibiotics to the treatment of infections in medical practice, the initial enthusiasm has substantially faded due to increasing drug resistance in pathogenic microorganisms. Therefore, there is a need for novel analytical and diagnostic methods in order to extend our knowledge regarding the mode of action of the conventional and novel antimicrobial agents from a perspective of single microbial cells as well as their communities growing in infected sites, i.e., biofilms. In recent years, atomic force microscopy (AFM) has been mostly used to study different aspects of the pathophysiology of noninfectious conditions with attempts to characterize morphological and rheological properties of tissues, individual mammalian cells as well as their organelles and extracellular matrix, and cells’ mechanical changes upon exposure to different stimuli. At the same time, an ever-growing number of studies have demonstrated AFM as a valuable approach in studying microorganisms in regard to changes in their morphology and nanomechanical properties, e.g., stiffness in response to antimicrobial treatment or interaction with a substrate as well as the mechanisms behind their virulence. This review summarizes recent developments and the authors’ point of view on AFM-based evaluation of microorganisms’ response to applied antimicrobial treatment within a group of selected bacteria, fungi, and viruses. The AFM potential in development of modern diagnostic and therapeutic methods for combating of infections caused by drug-resistant bacterial strains is also discussed.

show abstract

Adhesion of Legionella pneumophila on glass and plumbing materials commonly used in domestic water systems

Cited by 17 publications

References 38 publications

Environmental surveillance of Legionella pneumophila in hot water systems of hotels in Morocco

Environmental surveillance of Legionella pneumophila in hot water systems of hotels in Morocco

Adhesion Behavior of Escherichia coli Strains on Glass: Role of Cell Surface Qualitative and Quantitative Hydrophobicity in Their Attachment Ability

Physics Comes to the Aid of Medicine—Clinically-Relevant Microorganisms through the Eyes of Atomic Force Microscope

Contact Info

Product

Resources

About