Adhesion of <i>Candida albicans</i> to epithelial cells ‐ effect of nikkomycin

Segal, Esther; Gottlieb, S.; Altboum, Zeev; Gov, Yael; Berdicevsky, Israela

doi:10.1111/j.1439-0507.1997.tb00168.x

Cited by 9 publications

(9 citation statements)

References 18 publications

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“…These patients had received antifungal treatment, which according to several investigators reduces adhesion of this yeast to human cells significantly. This feature can be a basis for further research, once corroborate the prophylactic use of antifungal on risk populations 42–44 …”

Section: Discussionmentioning

confidence: 73%

Adherence of Candida albicans and Candida parapsilosis to epithelial cells correlates with fungal cell surface carbohydrates

Lima-Neto

Beltrão²,

Oliveira

et al. 2010

Mycoses

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Many studies have described the adherence of Candida albicans to epithelial cells but little is known about Candida parapsilosis adhesion and its role in host cell surface recognition. This study was designed to evaluate the correlation between the adherence of 20 C. albicans and 12 C. parapsilosis strains to human buccal epithelial cells and the expression of fungal cell surface carbohydrates using lectin histochemistry. Adherence assays were carried out by incubating epithelial cells in yeast suspensions (10(7) cells ml(-1) ) and peroxidase conjugated lectins (Con A, WGA, UEA I and PNA at 25 μg ml(-1) ) were used for lectin histochemistry. The results showed that adherence was overall greater for C. albicans than for C. parapsilosis (P < 0.01) and that the individual strain differences correlated with a high content of cell surface α-l-fucose residues as indicated by the UEA I staining pattern. Based on the saccharide specificity of the lectins used, these results suggest that l-fucose residues on cell surface glycoconjugates may represent recognition molecules for interactions between the yeast strain studied and the host (r = 0.6985, P = 0.0045). In addition, our results indicated the presence of α-d-glucose/α-d-mannose, N-acetyl-D-glucosamine/N-acetylneuraminic acid and D-galactose/N-acetyl-D-galactosamine in fungal cell wall.

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Section: Discussionmentioning

confidence: 73%

Adherence of Candida albicans and Candida parapsilosis to epithelial cells correlates with fungal cell surface carbohydrates

Lima-Neto

Beltrão²,

Oliveira

et al. 2010

Mycoses

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“…A chitin-soluble extract (CSE) also inhibited binding of C. albicans to human corneocytes (Kahana et al, 1988). C. albicans cells exposed to nikkomycin, a chitin synthetase inhibitor, had decreased chitin content and showed a corresponding decrease in adherence to BECs (Segal et al, 1997). The site of adherence between the yeast cells and epithelial cells labeled intensely with wheat germ agglutinin, a lectin-recognizing chitin.…”

Section: Removalmentioning

confidence: 99%

Oral Colonization By Candida Albicans

Cannon

Chaffin

1999

Critical Reviews in Oral Biology & Medicine

276

193

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Candida albicans is a commensal yeast normally present in small numbers in the oral flora of a large proportion of humans. Colonization of the oral cavity by C. albicans involves the acquisition and maintenance of a stable yeast population. Micro-organisms are continually being removed from the oral cavity by host clearance mechanisms, and so, in order to survive and inhabit this eco-system, C. albicans cells have to adhere and replicate. The oral cavity presents many niches for C. albicans colonization, and the yeast is able to adhere to a plethora of ligands. These include epithelial and bacterial cell-surface molecules, extracellular matrix proteins, and dental acrylic. In addition, saliva molecules, including basic proline-rich proteins, adsorbed to many oral surfaces promote C. albicans adherence. Several adhesins present in the C. albicans cell wall have now been partially characterized. Adherence involves lectin, protein-protein, and hydrophobic interactions. As C. albicans cells evade host defenses and colonize new environments by penetrating tissues, they are exposed to new adherence receptors and respond by expressing alternative adhesins. The relatively small number of commensal Candida cells in the oral flora raises the possibility that strategies can be devised to prevent oral colonization and infection. However, the variety of oral niches and the complex adherence mechanisms of the yeast mean that such a goal will remain elusive until more is known about the contribution of each mechanism to colonization.

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“…14 Actinomyces species can produce some anti-fungal material, including nikkomycin and geldanamycin. 15 Therefore, we speculated that oral Actinomyces could establish an antagonistic relationship with oral Candida.…”

Section: Introductionmentioning

confidence: 99%