Adhesion of hematopoietic progenitor cells to human bone marrow or umbilical vein derived endothelial cell lines

In vitro assessment of bone marrow endothelial colonies (CFU-En) in non-Hodgkin's lymphoma patients undergoing peripheral blood stem cell transplantation

“…3,4 Porcine microvascular endothelial cell stromal layers and embryonic aorta-gonadmesonephro region-derived endothelium are able to support the initial expansion of human hematopoietic progenitor cells, and have the ability to protect hematopoietic progenitors from extensive differentiation. [3][4][5] There is increasing evidence that EC residing in the BM can contribute to maintenance and proliferation of stem/ progenitor cells. They also seem to be implicated in the regulation of stem cells trafficking during progenitor cell mobilization and homing, but the mechanisms involved are not yet completely understood.…”

“…They also seem to be implicated in the regulation of stem cells trafficking during progenitor cell mobilization and homing, but the mechanisms involved are not yet completely understood. 5,6 There is also evidence for circulating EC that are probably mobilized in response to endogenous stimuli or exogenously by cytokine therapy. 7 However, most studies on stromal regulation of hematopoiesis derive from investigation of BM fibroblasts while few studies have investigated the in vitro characteristics and the role of human BM endothelial cells in hematological diseases.…”

Proteoglycans on bone marrow endothelial cells bind and present SDF-1 towards hematopoietic progenitor cells

“…It has been described by a number of groups that the firm adhesion step of HPC to BMEC or other endothelial cells is mediated via binding of the integrins very late activation antigen-4 (VLA-4), VLA-5 and leukocyte function-associated antigen-1 (LFA-1) on the HPC to their endothelial receptors, respectively VCAM-1 and intercellular adhesion molecule-1 (ICAM-1). [9][10][11] Recently, Peled et al 9,12 reported that the integrins VLA-4 and LFA-1 on umbilical cord blood CD34 + cells can be activated via signaling of the CXCR4 receptor when the chemokine stromal cell-derived factor-1 (SDF-1) is present on endothelial cells or integrin-binding substrates, resulting in firm adhesion and spreading of the cells.…”

The role of novel and known extracellular matrix and adhesion molecules in the homeostatic and regenerative bone marrow microenvironment

“…It is specifically co-expressed with CXCL12 in BM sinusoids and form targets of HSPC homing after transplantations. 162 E-selectin mediates adhesion of HSPCs to BM endothelial cells 164 and promotes HSC proliferation and maturation. 61 Knock-down of E-selectin or treatment of mice with an E-selectin antagonists enhanced HSC quiescence and self-renewal, which was partially mediated by interaction of PSGL-1 and HCELL on HSPCs with endothelial E-selectin.…”