Adhesion of culture cells to their substratum

Revel, Jean‐Paul; Hoch, Pamela; Ho, Dimitar

doi:10.1016/0014-4827(74)90398-x

Cited by 215 publications

(83 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…12) and are associated with the plasma membrane (13,14,27). In untransformed nonmuscle cells, actin-containing microfilaments have been implicated in a number of cellular functions such as cytokinesis, endocytosis and exocytosis, cell adhesion to a substratum, cell locomotion, membrane ruffling, and maintenance of cell shape (7,(27)(28)(29)(30)(31).…”

Section: Discussionmentioning

confidence: 99%

Changes in microfilament organization and surface topogrophy upon transformation of chick embryo fibroblasts with Rous sarcoma virus.

Wang¹,

Goldberg²

1976

Proc. Natl. Acad. Sci. U.S.A.

159

View full text Add to dashboard Cite

A series of morphological changes occurred when chick embryo fibroblasts infected with the NY68 mutant of Rous sarcoma virus were shifted from nonpermissive temperature (410) to permissive temperature (370). We observed three distinct stages in cell morphology and surface topography that were correlated with a reduction in the organization and assembly of actin-containing microfilament bundles. Our observations suggest that control of microfilament organization and surface topography are responsive to the presence of a functioning transforming gene (src) product of Rous sarcoma virus.Transformation of chick embryo fibroblasts by Rous sarcoma virus is independent of virus replication (1, 2) and is under the influence of a viral gene that is called the src gene because it contains sequences specific for sarcomagenesis (3). Cells infected with virus mutants that have a temperature-sensitive lesion in the src gene product are transformed at a permissive temperature but not at a nonpermissive temperature. Thermal denaturation of the src gene product of some of these mutants is reversible and thus provides a useful system for studying changes in cell metabolism that are associated with transformation.The presence of a functional src gene can be correlated with a broad array of alterations (2), many of which involve the membrane. Included in this category of membrane-associated phenomena in transformed cells are increased agglutinability with plant lectins, loss of surface proteins, changes in adhesion properties, changes in contact inhibition of growth and locomotion, changes in membrane transport, elevated levels of a protease that is a plasminogen activator, and changes in cell morphology. In particular, alteration in cell shape has been used as a convenient marker for assaying cellular transformation (4-6). Changes in the morphology and surface topography of mammalian cells are associated with the assembly and organization of cytoplasmic fibers such as microtubules, 100 A filaments, and microfilament bundles (7-9).The cytoskeleton of transformed cell lines seems to be altered (7, 9). Studies using fluorescent antibody directed against actin showed that the arrangement of actin filaments in cables is lost or much reduced in mouse and rat cells transformed by simian virus 40 (10-12). McNutt et al. (13,14) had drawn a similar conclusion from their earlier results using transmission electron microscopy. In the present study we describe changes in cell shape, topography, and cytoplasmic fibers in chick cells infected with Rous sarcoma virus that are associated with the action, either direct or indirect, of the src gene product. MATERIALS AND METHODSCells and Viruses. The growth and maintenance of normal and virus-infected chick embryo fibroblasts was as described (15). In all experiments, cultures were grown in Scherer's medium containing 10% tryptose phosphate broth and 5% calf serum. The temperature-sensitive transformation mutant NY68 of the Schmidt-Ruppin strain of Rous sarcoma virus was obtained from Drs. K...

show abstract

Section: Discussionmentioning

confidence: 99%

Changes in microfilament organization and surface topogrophy upon transformation of chick embryo fibroblasts with Rous sarcoma virus.

Wang¹,

Goldberg²

1976

Proc. Natl. Acad. Sci. U.S.A.

159

View full text Add to dashboard Cite

show abstract

“…Macrophages were collected and maintained in culture for [3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18] days on 25 mm round glass cover slips as previously described (17). There was considerable variability in cellular activity, as determined by extent of macrophage spreading and ruffling, between cultures prepared with different lots of serum.…”

Section: Cultivation Of Macrophagesmentioning

confidence: 99%

Cell shape changes induced by cationic anesthetics.

Rabinovitch¹,

DeStefano²

1976

The Journal of Experimental Medicine

View full text Add to dashboard Cite

Cell functions such as adhesion, movement, and phagocytosis involve changes in both cell shape and cell surface motility. These changes are the result of membrane perturbations caused by interactions of cells with substrates, particles, or other cells. Surface perturbations generate signals that are presumably transduced into changes in the state of aggregation and distribution of submembranous cytoskeletal constituents (1-3). Except for the role of Fc and C3 receptors in the interaction of particles or substrates with phagocytes (3), other cell surface-"sensing" components, as well as the nature of the membrane perturbations and the transducing mechanisms are unknown. Induced cell shape changes permit the analysis of factors involved in maintenance of cell shape and in cell motility. A few diverse agents promote rapid and reversible changes in cell shape. Examples are the spreading of cells induced by Mn 2÷ (4) and the cell rounding induced by high hydrostatic pressure or exposure to trypsin, strychnine, EDTA, or cytochalasins (5-9). Cationic anesthetics inhibit a variety of cell surface phenomena, including cell to substrate adhesion, spreading, phagocytesis, locomotion, cell fusion, capping of surface Ig, or cell mediated-cytotoxicity (10-16). We show here that exposure of cultivated macrophages to cationic anesthetics results in extensive and fully reversible contraction and rounding. Single cells were followed before, during, and after exposure to the drugs and the observations recorded in still pictures and time-lapse movies. We examined several features of anesthetic-induced rounding, investigated structure-activity relationships of the drugs, and compared anesthetic effects to those of EDTA. Materials and Methods

show abstract

“…In a previous paper, we used monoclonal antibodies (Mabs) ~ that specifically recognize carbohydrate determinants on the gangliosides GD2 and GD32 (8) to localize them on the surface of human melanoma cells and their focal adhesion plaques. The contention that gangliosides are indeed important molecules in cell-substratum interactions is strengthened by the fact that pretreatment of melanoma cells with specific Mabs directed to either GD2 or GD3 significantly decreased their ability to attach and spread on a variety of extracellular matrix proteins, which include fibronectin, vitronectin, laminin, and collagen (10).The attachment and spreading of cells on extracellular matrix components undoubtedly involve very complex interactions which include not only specific receptors (1,4,5,13,15, 27, 31,33,45,46) on the surface of the membrane but also cytoskeletal components beneath it (22,35,36). Components of substrate-attached cell adhesion plaques include cytoskeletal proteins (22, 35, 36) and proteoglycans (14, 21, …”

mentioning

confidence: 99%

“…The attachment and spreading of cells on extracellular matrix components undoubtedly involve very complex interactions which include not only specific receptors (1,4,5,13,15,27,31,33,45,46) on the surface of the membrane but also cytoskeletal components beneath it (22,35,36). Components of substrate-attached cell adhesion plaques include cytoskeletal proteins (22,35,36) and proteoglycans (14, 21, I.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Disialoganglioside GD2 distributes preferentially into substrate-associated microprocesses on human melanoma cells during their attachment to fibronectin.

Cheresh¹,

Klier²

1986

The Journal of Cell Biology

View full text Add to dashboard Cite

Abstract. Human melanoma cells (M21) actively attach and spread on a fibronectin substrate. Indirect immunofluorescence assays with specific monoclonal antibodies directed to the disialoganglioside GD2, the major ganglioside expressed on M21 melanoma cells, indicate that during the cell attachment process this molecule redistributes into microprocesses that make direct contact with the fibronectin substrate. Scanning and transmission immunoelectron microscopic studies with anti-GD2 monoclonal antibodies and immunogold staining demonstrate that GD2 preferentially localizes into substrate-associated microprocesses that emanate from the plasma membrane of the M21 cells.Staining with monoclonal antibodies directed to other melanoma surface antigens fails to demonstrate a similar distribution pattern on these cells. Direct evidence is provided that GD2 is involved in M21 cell attachment to fibronectin, since treatment of these cells with anti-GD2 monoclonal antibodies causes cell rounding and detachment from a fibronectin substrate. Moreover, scanning electron microscopy demonstrates that this loss of attachment of fibronectin is characterized by a perturbation of the cell attachment-promoting microprocesses that in the presence of these antibodies lose contact with the fibronectin substrate.umber of studies have indicated that the carbohydrate moiety of gangliosides plays a direct role in cellsubstratum interactions, thus implicating them as putative cell surface receptors for fibronectin and collagen (8,18,29,49). In a previous paper, we used monoclonal antibodies (Mabs) ~ that specifically recognize carbohydrate determinants on the gangliosides GD2 and GD32 (8) to localize them on the surface of human melanoma cells and their focal adhesion plaques. The contention that gangliosides are indeed important molecules in cell-substratum interactions is strengthened by the fact that pretreatment of melanoma cells with specific Mabs directed to either GD2 or GD3 significantly decreased their ability to attach and spread on a variety of extracellular matrix proteins, which include fibronectin, vitronectin, laminin, and collagen (10).The attachment and spreading of cells on extracellular matrix components undoubtedly involve very complex interactions which include not only specific receptors (1,4,5,13,15, 27, 31,33,45,46) on the surface of the membrane but also cytoskeletal components beneath it (22,35,36). Components of substrate-attached cell adhesion plaques include cytoskeletal proteins (22, 35, 36) and proteoglycans (14, 21,

show abstract

Adhesion of culture cells to their substratum

Cited by 215 publications

References 35 publications

Changes in microfilament organization and surface topogrophy upon transformation of chick embryo fibroblasts with Rous sarcoma virus.

Changes in microfilament organization and surface topogrophy upon transformation of chick embryo fibroblasts with Rous sarcoma virus.

Cell shape changes induced by cationic anesthetics.

Disialoganglioside GD2 distributes preferentially into substrate-associated microprocesses on human melanoma cells during their attachment to fibronectin.

Contact Info

Product

Resources

About