Adhesion of Blood Plasma Proteins and Platelet-rich Plasma on l-Valine-Based Poly(ester urea)

Abstract

The competitive absorption of blood plasma components including fibrinogen (FG), bovine serum albumin (BSA), and platelet-rich plasma (PRP) on l-valine-based poly(ester urea) (PEU) surfaces were investigated. Using four different PEU polymers, possessing compositionally dependent trends in thermal, mechanical, and critical surface tension measurements, water uptake studies were carried out to determine in vitro behavior of the materials. Quartz crystal microbalance (QCM) measurements were used to quantify the adsorption characteristics of PRP onto PEU thin films by coating the surfaces initially with FG or BSA. Pretreatment of the PEU surfaces with FG inhibited the adsorption of PRP and BSA decreased the absorption 4-fold. In vitro studies demonstrated that cells cultured on l-valine-based PEU thin films allowed attachment and spreading of rat aortic cells. These measurements will be critical toward efforts to use this new class of materials in blood-contacting biomaterials applications.

Preclinical in Vitro and in Vivo Assessment of Linear and Branched l-Valine-Based Poly(ester urea)s for Soft Tissue Applications

“…Thermogravimetric analyses (TGA) for linear PEUs and 2% branched PEUs (Figures SI3A and B) (Table ) show high degradation temperatures that make it suitable for compression mold processing. Poly­(1-VAL-12) shows a broader degradation temperature which is consistent with previously published work and could be attributed to greater chain flexibility which allows for more degradation processes . Values of T d are significantly higher than the reported T g values (Figure SI4).…”

“…Poly(1-VAL-12) shows a broader degradation temperature which is consistent with previously published work and could be attributed to greater chain flexibility which allows for more degradation processes. 39 Values of T d are significantly higher than the reported T g values (Figure SI4). As the methylene units within the polymer backbone increase, chain flexibility increases concomitantly, resulting in suppression of the T g with values ranging between 29 and 42 °C (Table 1).…”

Low-fouling, mixed-charge poly-l-lysine polymers with anionic oligopeptide side-chains

“…pH, ionic strength and temperature) play an important role in triggering protein-surface interactions. [10][11][12] Proteins show higher affinity for hydrophobic surfaces than hydrophilic ones, 10,13 and for rough substrates compared to flat substrates. 14 Biomaterial surfaces may be chemically treated to form antifouling layers capable of reducing or, ideally eliminating, fouling.…”

Platelet adhesion on commercially pure titanium plates in vitro I: effects of plasma components and involvement of the von Willebrand factor and fibronectin

“…Interestingly, adsorbed FGN (and vWF) can promote platelet adhesion more potently than FN [29], and it is adsorbed on the top of serum albumin in a bilayer or multilayer [23, 28]. Moreover, FGN stimulates platelet adhesion after being adsorbed onto a surface but not in its native form (nonadsorbed) [30], and it associates with TiO 2 markedly faster than human serum albumin [31].…”

“…Integrins function as dynamic scaffolds, which facilitate platelet adhesion onto surrounding cells or artificial surfaces, and as receptors for external signaling molecules, such as FGN, vWF, and FN [19, 24, 30, 32–36]. In this study, we examined platelet activation by the development of cytoskeletal actin fibers and the expression of surface markers.…”