Adhesion Molecules of Multipotent Mesenchymal Stromal Cells Obtained From Adipose Tissue During Simulation of the Effects of Microgravity

Ratushny, A. Yu.; Yakubets, D.A.; Zhivodernikov, I. V.; Буравкова, Л. Б.

doi:10.21687/0233-528x-2017-51-6-38-43

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“…No significant effects of 10-day SMG on the expression of stromal markers and cell adhesion molecules were found. Interestingly, that short-term (96 h) exposure of MSCs under SMG reduced the expression of CD105, CD51/61, CD44, and CD54 and altered the transcription activity of several genes associated with adhesion and extracellular matrix [ 42 ]. Probably, longer exposure allows MSCs to adapt to SMG and restore the normal expression of certain surface molecules.…”

Section: Resultsmentioning

confidence: 99%

“…Among the 16 ITG genes, only ITGA3 and ITGB1 were altered after 10 days of SMG. It should be mentioned that short-term SMG caused impaired cell adhesion in vitro and transcriptomic shifts of adhesion molecules [ 42 ]. It is known that each of the integrin subunits is encoded by an individual gene and, therefore, decreased expression of one integrin subunit can attenuate the cell–matrix interaction.…”

Section: Discussionmentioning

confidence: 99%

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Simulated Microgravity Remodels Extracellular Matrix of Osteocommitted Mesenchymal Stromal Cells

Zhivodernikov

Ratushnyy

Буравкова

2021

IJMS

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The extracellular matrix (ECM) is the principal structure of bone tissue. Long-term spaceflights lead to osteopenia, which may be a result of the changes in composition as well as remodeling of the ECM by osteogenic cells. To elucidate the cellular effects of microgravity, human mesenchymal stromal cells (MSCs) and their osteocommitted progeny were exposed to simulated microgravity (SMG) for 10 days using random positioning machine (RPM). After RPM exposure, an imbalance of MSC collagen/non-collagen ratio at the expense of a decreased level of collagenous proteins was detected. At the same time, the secretion of proteases (cathepsin A, cathepsin D, MMP3) was increased. No significant effects of SMG on the expression of stromal markers and cell adhesion molecules on the MSC surface were noted. Upregulation of COL11A1, CTNND1, TIMP3, and TNC and downregulation of HAS1, ITGA3, ITGB1, LAMA3, MMP1, and MMP11 were detected in RPM exposed MSCs. ECM-associated transcriptomic changes were more pronounced in osteocommitted progeny. Thus, 10 days of SMG provokes a decrease in the collagenous components of ECM, probably due to the decrease in collagen synthesis and activation of proteases. The presented data demonstrate that ECM-associated molecules of both native and osteocommitted MSCs may be involved in bone matrix reorganization during spaceflight.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%