Adhesion molecules and lymphocyte subsets in milk and blood of periparturient Holstein cows

Kampen, Corinne Van; Mallard, Bonnie A.; Wilkie, B.N.

doi:10.1016/s0165-2427(99)00034-3

Cited by 35 publications

(28 citation statements)

References 18 publications

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“…The blood leukocyte subsets data from PO-fed group confirm reports in the literature, where a general predominance of CD4-positive cells over CD8-positive cells was reported, with a non-significant increase at 4 DIM that returned to approximately the initial value at 15 DIM. FO-fed group showed generally stable numbers of CD8-positive cells; these data confirm the findings of previous studies in dairy cattle regarding the quantity of leukocytes in each subset, but not the general decreasing trend in leukocyte subsets associated with parturition (Van Kampen et al, 1999;Harp et al, 2004). The CD4/CD8 ratio was generally stable in PO-fed group, while FO-fed group showed a constant increase in CD4 cells relative to CD8 cells according with Kimura et al (1999).…”

Section: Discussionsupporting

confidence: 88%

Effects of protected fish oil in the diet of periparturient dairy goats on phenotypic variation in blood and milk leukocytes

Bronzo

Puricelli

Agazzi

et al. 2010

Animal

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The goal of this study was to evaluate the effects of dietary protected fish oil (FO) on phenotypic variation in blood, milk leukocytes, and some productive and metabolic parameters in periparturient dairy goats. About 12 Alpine goats, selected from a larger group of second-parity animals, were fed from 15 days before kidding until the 15th day of lactation with the same basal diet that had been supplemented with either 47 g/head per day of FO or 47 g/head per day hydrogenated palm oil (PO). Dry matter intake, live body weight (LBW), body condition score (BCS), and productive performance were evaluated in 2 weeks after kidding. On days 15, 7, and 2 before kidding and days 2, 7, and 15 after kidding, plasma samples were collected for evaluation of alanine aminotransferase, aspartate aminotransferase, non-esterified fatty acids, glucose, beta-hydroxybutyrate, cholesterol, and urea levels. White blood cell and blood leukocyte subsets were counted in whole blood samples on the kidding day, as well as at 1, 4, and 15 days after kidding. In addition, milk somatic cell count, intramammary infection (IMI), and milk leukocyte subsets were evaluated on days 4 and 15 after kidding. No differences were observed in dry matter intake and BCS, while LBW was higher in FO-fed animals. Milk production and composition, plasma metabolites, and liver enzymes were similar in both experimental groups. Blood CD4 positive cells increased constantly ( P 5 0.05) in FO-fed group, while CD8 and CD14 cell counts significantly increased 4 days after kidding ( P , 0.01). Milk leukocyte subsets showed a significant ( P , 0.01) decrease in PO-fed group and a nonsignificant increase ( P 5 0.34) in FO-fed group, despite the presence of coagulase negative staphylococci IMI. The results of the productive performance evaluation agreed with those of many other studies, which did not find any significant differences between dairy goats fed diets enriched with FO or PO supplements. The administration of FO to dairy goats in transition appeared to affect the variation in blood leukocytes with a constant increase in CD4-and CD8-positive cells in comparison with a PO fat-supplemented diet.

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Section: Discussionsupporting

confidence: 88%

Effects of protected fish oil in the diet of periparturient dairy goats on phenotypic variation in blood and milk leukocytes

Bronzo

Puricelli

Agazzi

et al. 2010

Animal

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“…Migration of activated T cells into the gland lumen of sheep can be induced by infusion with lipopolysaccharide (LPS) or with parasite larvae [26]. In general, the distribution of lymphocyte subsets in the mammary gland is strongly influenced by lactation and gestation stage [95,102]. Furthermore, the lactating mammary gland also seems to specifically influence the T cell subset composition in blood after parturition -an effect which can be abolished by mastectomy [36].…”

Section: Mammary Glandmentioning

confidence: 99%

Lymphocyte migration studies

Bimczok

Rothkötter

2006

Vet. Res.

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-For maintenance of immunity and tolerance, the organs and tissues of the organism are connected by migrating lymphoid cells. Understanding lymphocyte migration is essential for many disorders and diseases -especially in the mucosa-lined organs. Detailed analyses of migrating lymphocytes have been performed in many species, especially in laboratory animals. However, important experiments in lymphocyte migration have been carried out in large animals, for example sheep, cattle and pigs. These species allow experimental procedures like in situ-organ labelling, lymphocyte retransfusion studies or lymph vessel cannulations. Such studies have made an important contribution to the understanding of the overall principles of lymphocyte migration especially in the mucosal immune system. Major results on the specific migration of naïve and memory T cells through lymphoid organs, the re-distribution of γ/δ T cells in the intestinal immune system and the emigration of newly produced B cells from the ileal Peyer's patches have been obtained in large animals. Since there are growing numbers of markers for large animals, and molecular biology methods are available in these species, experiments in large animals will be an essential tool for the understanding of lymphocyte migration especially in mucosal organs.

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“…Our results concerning B-lymphocytes agree with others (Taylor et al, 1994;Harp et al, 2004), as T-lymphocytes traffic selectively into bovine mammary gland secretions whereas B-lymphocytes represent a minor population as compared with peripheral blood. In general, the composition of the lymphoid compartment in the lactating mammary gland differs from that in peripheral blood, as demonstrated in the present and other studies (Park et al, 1992;Wirt et al, 1992;Van Kampen et al, 1999). Furthermore, the percentages of the different CMC subpopulations depend on differences in the techniques used to identify them (Prescott and Breed, 1910;Concha et al, 1980;Nonnecke and Kehrli, 1985), the gating strategy (Ohtsuka et al, 2010), the definition of colostrum (Archambault et al, 1988a), and the health status of the udder (Kiku et al, 2010).…”

Section: Discussionmentioning

confidence: 48%

Effect of β-hydroxybutyric acid, parity, and body condition score on phenotype and proliferative capacity of colostral mononuclear leukocytes of high-yielding dairy cows

Meganck

Goddeeris

Campeneere³

et al. 2015

Journal of Dairy Science

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In neonatal calves, the ingestion of colostrum is imperative for preventing infectious diseases. Investigations into the transfer of passive immunity of cattle have primarily focused on the importance of colostral immunoglobulins, with a recent increase in focus on understanding the role of colostral leukocytes. The main objective of the present study was to measure the influence of parity, body condition score, serum nonesterified fatty acids, and serum β-hydroxybutyrate concentrations of periparturient cows on phenotype and mitogen-and antigen-induced proliferative capacity of bovine colostral leukocytes. Holstein-Friesian cows (n = 141) were intramuscularly vaccinated at 60 and 30 d before the expected parturition date with a tetanus toxoid vaccine. Of these 141 animals, 28 primiparous and 72 multiparous cows were sampled immediately after parturition. Colostrum mononuclear cell populations were identified by flow cytometry using bovine cluster of differentiation markers, and the proliferative capacity of these cells was determined using a 3 H-thymidine proliferation assay. Under-conditioned cows had a significantly higher percentage of colostral macrophages than normal-conditioned animals, whereas over-conditioned cows had significantly more colostral B-lymphocytes. Serum β-hydroxybutyrate was significantly associated with higher numbers of colostral T-lymphocytes and macrophages. Heifers had significantly higher mitogen-and antigen-induced proliferation of their colostral leukocytes than third parity or older cows. In conclusion, body condition score, parity, and serum β-hydroxybutyrate concentration of periparturient high-yielding dairy cows were shown to influence the number of colostral macrophages or the mitogen-and antigen-induced proliferation of colostral leukocytes, possibly influencing the cellular immunity of the newborn calf.

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Adhesion molecules and lymphocyte subsets in milk and blood of periparturient Holstein cows

Cited by 35 publications

References 18 publications

Effects of protected fish oil in the diet of periparturient dairy goats on phenotypic variation in blood and milk leukocytes

Effects of protected fish oil in the diet of periparturient dairy goats on phenotypic variation in blood and milk leukocytes

Lymphocyte migration studies

Effect of β-hydroxybutyric acid, parity, and body condition score on phenotype and proliferative capacity of colostral mononuclear leukocytes of high-yielding dairy cows

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