Adhesion Molecule Polymorphisms in Acute Renal Allograft Rejection

Khazen, D.; Jendoubi-Ayed, S.; Gorgi, Yousr; Sfar, Imen; Abderrahim, E.; Abdallah, T. Ben; Ayed, K.

doi:10.1016/j.transproceed.2007.08.031

Cited by 14 publications

(11 citation statements)

References 8 publications

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“…The prevalence of ICAM1 gene polymorphisms genotype (GG:GA:AA) in our controls was in complete agreement with previously published reports for the Caucasian population (34) and for Tunisian population (35). One other possible explanation for this conflict might be the small sample size in these studies (13, 31, 32).…”

Section: Clinical Characteristics Of Patients With Behçet's Disease Asupporting

confidence: 92%

Intercellular adhesion molecule 1 K469E gene polymorphism is associated with presence of skin lesions in Tunisian Behçet's disease patients

et al. 2009

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Intercellular adhesion molecule 1 (ICAM1) gene polymorphisms have been implicated in the susceptibility to inflammatory diseases. The expression of both soluble and tissue ICAM1 were increased in Behçet's disease (BD) but the contribution of ICAM1 gene polymorphisms to this disease remains unknown. We sought to establish the association of ICAM1 gene K469E polymorphism in exon 6 with susceptibility for BD. One hundred and thirty-five Tunisian patients who satisfied the International Study Group criteria for BD and 157 healthy blood donor controls from the same geographic area were genotyped by polymerase chain reaction method for the K469E ICAM1 gene polymorphisms in exon 6. There were no significant differences in the distribution of the K469E allele or genotype frequencies between the BD patients and healthy controls in the ICA1 gene. Among patients, significant association was found between the presence of skin lesions and the studied polymorphism in the distribution of the K469E allele (P = 0.004; odds ratio = 1.26; 95% confidence interval = 2.13-3.62) and genotype frequencies (P = 0.0028; chi(2) = 11.75). Our findings suggest that K469E ICAM1 gene polymorphism was associated with Tunisian BD patients with skin lesions.

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Section: Clinical Characteristics Of Patients With Behçet's Disease Asupporting

confidence: 92%

Intercellular adhesion molecule 1 K469E gene polymorphism is associated with presence of skin lesions in Tunisian Behçet's disease patients

et al. 2009

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“…Worldwide interest in this group of antigens has been generated in the last few years because several reports concur on its major role in immune complications such as hematopoietic stem cell transplantation (HSCT) outcomes; graft rejection of HLA‐matched solid organ transplants; and foeto‐maternal allo‐immunization (4–12). HA‐1 and HA‐2 are the two human MiHAgs recognized by their restricted expression to hematopoietic and leukemic cells (13, 14).…”

Section: Ha‐1 and Ha‐2 Genotype Frequencies In Tunisiansmentioning

confidence: 99%

HA-1 and HA-2 minor histocompatibility antigens in Tunisians

Sellami¹,

Ahmed²,

Kâabi³

et al. 2010

Tissue Antigens

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Minor histocompatibility antigens (MiHAgs), such as HA-1 and HA-2, are the main targets of immune responses after allogeneic stem cell transplantation (SCT). HA-1 and HA-2 are two hematopoietic system-restricted antigens encoded, respectively, by HMHA1 and MYO1G genes. In order to estimate their frequencies in Tunisians, we performed a molecular-based allele analysis for 160 healthy and unrelated subjects. Genomic DNAs were extracted mainly by the salting out method. Single nucleotide polymorphism (SNP) genotyping assays for selected sites at HMHA1 gene (rs3764653 and rs1801284) and at MYO1G gene (rs61739531) were performed with a sequence specific primers-polymerase chain reaction (SSP-PCR) method. Statistical analysis of our results showed that the HA-2 antigen is more frequent than the HA-1 antigen in the Tunisian population because their frequencies were 97% and 57%, respectively. Allele analysis for HMHA1 gene showed that the R variant (500T-504G) was predominant in our population (64%). For the MYO1G gene, the C allele was predominant (84%). All loci were in Hardy-Weinberg equilibrium (minimum P value = 0.06). Our frequencies were close to those reported in African and Caucasian groups.

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“…This study clearly demonstrates that increased immunogenicity after ischemia-reperfusion stress is an integral part of renal HSR. Based on studies of MHC class II and ICAM-1 as key markers of renal immunogenicity (Khazen et al 2007;Mendoza-Carrera et al 2008;Shackleton 1998;Shoskes and Halloran 1991) and Hsp70 as the key marker and HSF-1 as the key regulator of the renal HSR (Shamovsky and Nudler 2008;Pirkkala et al 2001), our data show that HSF-1 plays a role in the concordant upregulation of tubular immunogenicity and HSR, representing a direct molecular link between ischemia-reperfusion injury and renal immunogenicity.…”

Section: Discussionmentioning

confidence: 71%

“…Restitution of blood flow with reperfusion sustains renal allograft injury by further generation of free oxygen radicals. Ischemia-reperfusion and the associated oxidative stress not only disrupt cellular homeostasis but also increase the expression of adhesion molecules such as intercellular adhesion molecule-1 (ICAM-1) and major histocompatibility complex (MHC) classes I and II in interstitial cells and importantly also in renal tubular cells, thereby increasing immunogenicity and risk for allograft rejection (Mendoza-Carrera et al 2008;Khazen et al 2007;Shackleton et al 1990;Shoskes et al 1990;Shackleton 1998). However, the direct molecular link between renal ischemia-reperfusion injury and tubular immunogenicity is still unknown.…”

Section: Introductionmentioning

confidence: 99%

Increased immunogenicity is an integral part of the heat shock response following renal ischemia

Bidmon

Kratochwill

Rusai

et al. 2012

Cell Stress and Chaperones

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Renal ischemia increases tubular immunogenicity predisposing to increased risk of kidney allograft rejection. Ischemia-reperfusion not only disrupts cellular homeostasis but also induces the cytoprotective heat shock response that also plays a major role in cellular immune and defense processes. This study therefore tested the hypothesis that upregulation of renal tubular immunogenicity is an integral part of the heat shock response after renal ischemia. Expressions of 70 kDa heat shock protein (Hsp70), major histocompatibility complex (MHC) class II, and intercellular adhesion molecule-1 (ICAM-1) were assessed in normal rat kidney (NRK) cells following ATP depletion (antimycin A for 3 h) and heat (42°C for 24 h). In vitro, transient Hsp70 transfection and heat shock factor-1 (HSF-1) transcription factor decoy treatment were performed. In vivo, ischemic renal cortex was investigated in Sprague-Dawley rats following unilateral renal artery clamping for 45 min and 24 h recovery. Upregulation of Hsp70 was closely and significantly correlated with upregulation of MHC class II and/or ICAM-1 following ATP depletion and heat injury. Bioinformatics analysis searching the TRANSFAC database predicted HSF-1 binding sites in these genes. HSF-1 decoy significantly reduced the expression of immunogenicity markers in stressed NRK cells. In the in vivo rat model of renal ischemia, concordant upregulation of MHC class II molecules and Hsp70 suggests biological relevance of this link. The results demonstrate that upregulation of renal tubular immunogenicity is an integral part of the heat shock response after renal ischemia. Bioinformatic analysis predicted a molecular link to tubular immunogenicity at the level of the transcription factor HSF-1 that was experimentally verified by HSF-1 decoy treatment. Future studies in HSF-1 knockout mice are needed.

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Adhesion Molecule Polymorphisms in Acute Renal Allograft Rejection

Cited by 14 publications

References 8 publications

Intercellular adhesion molecule 1 K469E gene polymorphism is associated with presence of skin lesions in Tunisian Behçet's disease patients

Intercellular adhesion molecule 1 K469E gene polymorphism is associated with presence of skin lesions in Tunisian Behçet's disease patients

HA-1 and HA-2 minor histocompatibility antigens in Tunisians

Increased immunogenicity is an integral part of the heat shock response following renal ischemia

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