Adhesion molecule expression in local-macrophage-depleted rats bearing orthotopic corneal allografts

Slegers, Tanja P. A. M.; Veen, Gerard van; Hermans, Liesbeth; Broersma, Lourens; Rooijen, Nico van; Völker-Dieben, H. J. M.; Rij, Gabriël van; Gaag, R van der

doi:10.1007/s00417-003-0665-1

Cited by 5 publications

(2 citation statements)

References 20 publications

(19 reference statements)

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“…Unfortunately, many cell types can be affected by clodronate, including endothelial cells (31), so the relative role of lung macrophages remains unclear. This compound also has additional effects in the lung, including the following: inhibition of matrix metalloproteinases (32,33), altered hematopoiesis (34), and blunted expression of both intercellular adhesion molecule-1 and ␤ 2 -integrin (35). Furthermore, because gain of function was not demonstrated in this study (29), it is possible that macrophages must act in combination with other cell types to facilitate neutrophilic recruitment to the lung.…”

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confidence: 90%

The Critical Role of Hematopoietic Cells in Lipopolysaccharide-induced Airway Inflammation

Hollingsworth

Chen

Brass

et al. 2005

Am J Respir Crit Care Med

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Rapid and selective recruitment of neutrophils into the airspace in response to LPS facilitates the clearance of bacterial pathogens. However, neutrophil infiltration can also participate in the development and progression of environmental airway disease. Previous data have revealed that Toll-like receptor 4 (tlr4) is required for neutrophil recruitment to the lung after either inhaled or systemically administrated LPS from Escherichia coli. Although many cell types express tlr4, endothelial cell expression of tlr4 is specifically required to sequester neutrophils in the lung in response to systemic endotoxin. To identify the cell types requiring trl4 expression for neutrophil recruitment after inhaled LPS, we generated chimeric mice separately expressing tlr4 on either hematopoietic cells or on structural lung cells. Neutrophil recruitment into the airspace was completely restored in tlr4-deficient mice receiving wild-type bone marrow. By contrast, wild-type animals receiving tlr4-deficient marrow had dramatically reduced neutrophil recruitment. Moreover, adoptive transfer of wild-type alveolar macrophages also restored the ability of tlr4-deficient recipient mice to recruit neutrophils to the lung. These data demonstrate the critical role of hematopoietic cells and alveolar macrophages in initiating LPS-induced neutrophil recruitment from the vascular space to the airspace.

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confidence: 90%

The Critical Role of Hematopoietic Cells in Lipopolysaccharide-induced Airway Inflammation

Hollingsworth

Chen

Brass

et al. 2005

Am J Respir Crit Care Med

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“…Several adhesion molecules, including LFA-1 and ICAM, have been implicated in corneal graft rejection. [9][10][11] In this study, we used array technology to investigate the changes in the expression of genes involved in cell interactions and adhesion in peripheral blood at the time of corneal graft rejection episodes. Identifying such genes may further advance our knowledge of the processes involved in graft rejection and provide useful prognostic markers of graft rejection…”

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confidence: 99%

Increased Rac2 mRNA expression in peripheral blood during human corneal graft rejection

Huang

Tullo

Hillarby

2008

Eye

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Purpose Allograft rejection is the main cause of graft failure in human corneal transplantation, for which underlying pathomechanism is not yet clear. We compared gene expression in the peripheral blood of patients who after undergoing corneal transplantation experienced graft rejection with those patients who accepted grafts. Methods Sixty-six patients who underwent corneal transplantation were studied including 18 patients who suffered subsequent graft rejection. cDNA array technology was used to survey and quantify transcript expression . A semiquantitative reverse transcriptase-PCR (RT-PCR) was used to confirm the gene expression pattern measured by a cDNA array of selected genes. Results Among 265 genes present on the array, eight genes were found to be differentially expressed. Four genes (Rac 2, RhoA, paxillin, and CD18) were further analysed by semiquantitative RT-PCR, and significant differences in mRNA expression levels in the rejection group were confirmed. Conclusions Our study demonstrated that the expression of Rac2 mRNA was upregulated in the peripheral blood of patients experiencing corneal transplantation rejection compared to those patients who had no rejection episodes. In addition, three genes, RhoA, paxillin, and CD18, showed decreased expression in rejecting patients. cDNA array technology provides a potentially useful approach to identify novel genes that might participate in pathogenic pathways during corneal graft rejection.

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New Developments in Topical and Systemic Immunomodulation Following Penetrating Keratoplasty

Reis¹,

Reinhard

2010

Essentials in Ophthalmology

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Adhesion molecule expression in local-macrophage-depleted rats bearing orthotopic corneal allografts

Abstract: Prolonged corneal graft survival in rats, obtained via local depletion of macrophages, correlates with diminished expression of adhesion molecules.

Cited by 5 publications

References 20 publications

The Critical Role of Hematopoietic Cells in Lipopolysaccharide-induced Airway Inflammation

The Critical Role of Hematopoietic Cells in Lipopolysaccharide-induced Airway Inflammation

Increased Rac2 mRNA expression in peripheral blood during human corneal graft rejection

New Developments in Topical and Systemic Immunomodulation Following Penetrating Keratoplasty

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