Adhesion forces between AFM tips and superficial dentin surfaces

Pelin, Irina M.; Piednoir, Agnès; Machon, Denis; Farge, Pierre; Pirat, Christophe; Ramos, S.M.M.

doi:10.1016/j.jcis.2012.03.013

Cited by 24 publications

(20 citation statements)

References 32 publications

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“…Adhesion of bacteria to the tooth's surface is a prerequisite for biofilm formation [Chau et al, 2014]. Gaining an understanding of the adhesion force of dental biofilms to enamel is one key to the development of fundamental knowledge of dental caries [Pelin et al, 2012]. As shown in Figure 4, it suggested that adhesion force is positively related to rnc expression.…”

Section: The Weakened Biofilm Forming Ability Was Associated With a Rmentioning

confidence: 99%

The Regulator Gene rnc Is Closely Involved in Biofilm Formation in Streptococcus mutans

Mao

Lei

et al. 2018

Caries Res

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Streptococcus mutans is an important factor in the etiology and pathogenesis of dental caries, largely owing to its ability to form a stable biofilm. Previous animal studies have indicated that rnc could decrease the amount of sulcal caries, and that the downregulation of cariogenicity might be due to its capacity to disrupt biofilm formation. However, the biofunctions by which rnc is involved in biofilm formation remain to be elucidated. In this study, we further investigate the role of rnc based on the study of mature biofilm. Scanning electron microscopy and the crystal violet assay were used to detect the biofilm forming ability. The production and distribution of exopolysaccharides within biofilm was analyzed by exopolysaccharide staining. Gel permeation chromatography was used to perform molecular weight assessment. Its adhesion force was measured by atomic force microscopy. The expression of biofilm formation-associated genes was analyzed at the mRNA level by qPCR. Here, we found that rnc could occur and function in biofilm formation by assembling well-structured, exopolysaccharide-encased, stable biofilms in S. mutans. The weakened biofilm forming ability of rnc-deficient strains was associated with the reduction of exopolysaccharide production and bacterial adhesion. Over all, these data illustrate an interesting situation in which an unappreciated regulatory gene acquired for virulence, rnc, most likely has been coopted as a potential regulator of biofilm formation in S. mutans. Further characterization of rnc may lead to the identification of a possible pathogenic biofilm-specific treatment for dental caries.

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Section: The Weakened Biofilm Forming Ability Was Associated With a Rmentioning

confidence: 99%

The Regulator Gene rnc Is Closely Involved in Biofilm Formation in Streptococcus mutans

Mao

Lei

et al. 2018

Caries Res

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“…Todos os condicionadores ácidos utilizados apresentaram ácido fosfórico em concentrações que variavam de 30 a 40%, quantidade que se tem demonstrado efetiva na produção de padrão adequado de condicionamento da dentina 6,13,14 . Além da concentração do condicionador ácido, o tempo de condicionamento também teve importante papel sobre o padrão de condicionamento da dentina 15 , sendo que o tempo de 15 segundos parece ser o ideal 13,16 .…”

Section: Discussionunclassified

“…Essas alterações possibilitam a penetração do adesivo nas porosidades do esmalte, formando a zona de interdifusão, responsável pela união 1,2,5 . Diversamente, na dentina, o condicionamento remove a smear layer (formada durante o preparo cavitário), alarga a entrada dos túbulos dentinários e desmineraliza as dentinas peri e intertubular, expondo a rede de fibrilas colágenas 6,7 . A penetração do adesivo por entre as fibrilas colágenas forma a camada híbrida responsável pela união do adesivo ao tecido dentinário 2,6,8,9 .…”

Section: Introductionunclassified

Influência da marca do condicionador ácido na resistência de união da resina composta à dentina

Passos

Santos

Rodrigues

et al. 2012

Rev. odontol. UNESP

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ResumoIntrodução: Apesar da importância da etapa de condicionamento ácido nos procedimentos adesivos, pouco se sabe sobre o efeito da marca do condicionador ácido sobre a união do adesivo à dentina. Objetivo: Este estudo avaliou o efeito de diferentes condicionadores ácidos na união da resina composta à dentina. Material e método: Vinte e cinco incisivos bovinos tiveram a coroa dividida em duas hemissecções. As superfícies vestibulares foram desgastadas com lixa carbeto de silício (SiC) #180 até a exposição de dentina, seguida por lixas SiC #600 para padronização da smear layer. As amostras foram aleatoriamente alocadas em cinco grupos (n = 10), de acordo com marca do condicionador ácido: 3M ESPE, Ultradent, Dentsply, Villevie e DFL. Apenas um sistema de união convencional de dois passos (Ambar, FGM) foi utilizado. Matrizes de silicone foram utilizadas na confecção de três cilindros de resina composta em cada amostra. Após 24 horas, os cilindros foram testados sob carga de cisalhamento até a falha. Os dados foram analisados com ANOVA a um critério e teste de Tukey (α = 0,05). O modo de falha foi avaliado em microscopia óptica e os dados submetidos ao Teste Qui-quadrado (α = 0,05). Resultado: Não houve diferença estatística na resistência de união obtida entre os condicionadores ácidos avaliados. Houve predomínio de falhas adesivas, exceto para os condicionadores da Dentsply e DFL, que demonstraram similaridade entre falhas adesivas e mistas. Conclusão: As diferentes marcas de condicionadores ácidos avaliados não influenciaram a resistência de união de resina composta à dentina.Descritores: Adesivos dentinários; resistência ao cisalhamento; ataque ácido dentário. AbstractIntroduction: Despite the importance of the acid etching step on bonding procedures, little is known about the acid etchant brand effect on bond strength of adhesive to dentin. Objective: The aim of this study was to evaluate the effect of different acid etchants at the bond strength of composite resin to dentine. Material and method: Twenty five bovine incisors had their crown sectioned in two hemi-sections. The buccal surfaces were grounded with #180 silicon carbide (SiC) abrasive paper to exposure of dentin, followed by #600 SiC paper to standardize the smear layer. The samples were randomly allocated into five groups (n = 10) according to acid etchant brands: 3M ESPE, Ultradent, Dentsply, Villevie and DFL.Only a two-step etch-and-rinse adhesive system (Ambar, FGM) was used. Silicon molds were used to build-up three resin composite cylinder per sample. After 24 hours, cylinders were submitted to shear load until the failure. Data were analyzed by one way ANOVA and Tukey`s test (α = 0.05). The failure mode was evaluated under optical microscopy and the data were submitted to Chi-square test (α = 0.05). Result: There was no statistical difference on bond strength obtained between the acid etchants evaluated. There was predominance of adhesive failures, except for etchants of Dentsply and DFL that showed similarity between mixed and adhesive fail...

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“…ese methods have also been used to evaluate the surface morphology [7]. AFM provides three-dimensional detailed topographical images of surface roughness on a nanometer scale and has been used in dental research [8][9][10][11][12][13][14]. Roughness could be defined as a complex role of irregularities or little indentations that characterize a surface and influence on, inter alia, metalceramic bond quality [15].…”

Section: Introductionmentioning

confidence: 99%

Effect of Recasted Material Addition on the Quality of Metal‐Ceramic Bond: A Macro‐, Micro‐, and Nanostudy

Beer–Lech

Pałka

Skic

et al. 2018

Advances in Materials Science and Engineering

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Using the recasted alloys in dental prosthetics could affect the quality of the metal-ceramic bond. However, scientists, alloys producers, and prosthetists are still of different opinions. e purpose of this study was to estimate the influence of recasting of the NiCrMo alloy on the metal-ceramic bond quality. e research was carried out on macro-, micro-and nanoscales using the threepoint bending test procedure and hardness tests as well as atomic force microscopy and SEM analyses. e SEM analyses showed good integrity of the metal-ceramic bond. e τ b index of all test samples was greater than 45 MPa. e highest values were recorded for the samples made of 50% and 100% of a brand new material. SEM analysis made after the bending test confirmed good metal-ceramic bond and exhibited adhesive-cohesive fracture. e largest hardness of metal plates was found for the samples containing 50% of the recycled material. Atomic force microscopy studies showed that the alloy containing 50% of the recycled material was characterized by the highest values of surface roughness parameters.

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Adhesion forces between AFM tips and superficial dentin surfaces

Cited by 24 publications

References 32 publications

The Regulator Gene <b><i>rnc</i></b> Is Closely Involved in Biofilm Formation in <b><i>Streptococcus mutans</i></b>

The Regulator Gene <b><i>rnc</i></b> Is Closely Involved in Biofilm Formation in <b><i>Streptococcus mutans</i></b>

Influência da marca do condicionador ácido na resistência de união da resina composta à dentina

Effect of Recasted Material Addition on the Quality of Metal‐Ceramic Bond: A Macro‐, Micro‐, and Nanostudy

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