Adhesion force measurement of live cypris tentacles by scanning probe microscopy in seawater

Shiomoto, Shohei; Yamaguchi, Yuka; Yamaguchi, Kazuo; Nogata, Yasuyuki; Kobayashi, Motoyasu

doi:10.1038/s41428-018-0120-0

Cited by 8 publications

(9 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A typical strategy for the immobilization of water-soluble polymers is based on the introduction of water-insoluble units into the polymer, which act as an anchor to the support. − ,, Other strategies are based on covalent grafting techniques using certain initiators for polymerization or at specific reactive sites introduced at the support surface, called “grafting from” and “grafting to” methods, respectively. ,− These methods are based on precise chemical synthesis, and various kinds of grafting surface modifiers for polymers have been developed hitherto. However, such methods applicable to versatile polymeric materials are still quite limited.…”

Section: Introductionmentioning

confidence: 99%

A Facile Surface Functionalization Method for Polymers Using a Nonsolvent

Hirata¹,

Taneda²,

Nishio³

et al. 2020

ACS Appl. Bio Mater.

View full text Add to dashboard Cite

Surface treatment of polymeric solids without impairing their bulk properties is a crucial functionalization strategy for the promotion of their wider application. We here propose a facile method using a nonsolvent which can subtly alter or swell the polymer surface to be modified. A thin film of poly(methyl methacrylate) (PMMA) was immersed in a methanol solution of poly(2methoxyethyl acrylate) (PMEA). Electron spectroscopy for chemical analysis and neutron reflectometry revealed that a PMEA layer formed on the PMMA film with a diffused interface. The PMEA layer was very swollen in water and exhibited the ability to suppress serum protein adsorption and platelet adhesion on it. The functionalization technique using a nonsolvent was also applicable to the surface of other polymeric solids such as polyurethane.

show abstract

Section: Introductionmentioning

confidence: 99%

A Facile Surface Functionalization Method for Polymers Using a Nonsolvent

Hirata¹,

Taneda²,

Nishio³

et al. 2020

ACS Appl. Bio Mater.

View full text Add to dashboard Cite

show abstract

“…Recently, Shiomoto et al immobilized live cyprids on AFM cantilevers, and the adhesion force of a cyprid’s tentacles was determined by measuring the torsion of the cantilever when the tentacles were detached from glass surfaces coated with polymer brushes. 50…”

Section: Introductionmentioning

confidence: 99%

“…Recently, Shiomoto et al immobilized live cyprids on AFM cantilevers, and the adhesion force of a cyprid's tentacles was determined by measuring the torsion of the cantilever when the tentacles were detached from glass surfaces coated with polymer brushes. 50 In our previous work, we reported on the active marine antifouling performance of three sulfobetaine-based systems. 11 Bearing the same sulfobetaine group (−NH 2 + −(CH 2 ) 3 − SO 3 − ), the systems were characterized by the differences in linkage groups between the zwitterion and brush backbone.…”

Section: ■ Introductionmentioning

confidence: 99%

“…A quantitative assessment of interactions of cyprid footprint proteins with surfaces was achieved by colloidal probe-based AFM force spectroscopy. , Briefly, the footprint proteins were covalently attached by chemical coupling to previously chemically activated colloids of AFM probes, and the adhesion strength of the proteins against the surfaces was measured. Recently, Shiomoto et al immobilized live cyprids on AFM cantilevers, and the adhesion force of a cyprid’s tentacles was determined by measuring the torsion of the cantilever when the tentacles were detached from glass surfaces coated with polymer brushes …”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Brush Swelling and Attachment Strength of Barnacle Adhesion Protein on Zwitterionic Polymer Films as a Function of Macromolecular Structure

et al. 2019

View full text Add to dashboard Cite

The exceptional hydration of sulfobetaine polymer brushes and their resistance toward nonspecific protein absorption allows for the construction of thin films with excellent antibiofouling properties. In this work, swollen sulfobetaine brushes, prepared by surface-initiated atom transfer radical polymerization of two monomers, differentiated by the nature of the polymerizable group, are studied and compared by a liquid-cell atomic force microscopy technique and spectroscopic ellipsometry. Colloidal AFM-based force spectroscopy is employed to estimate brush grafting density and characterize nanomechanical properties in salt water. When the ionic strength-induced swelling behaviors of the two systems are compared, the differences observed on the antipolyelectrolyte response can be correlated with the stiffness variation on brush compression, likely to be promoted by solvation differences. The higher solvation of amide groups is proposed to be responsible for the lower adhesion force of the barnacle cyprid’s temporary adhesive proteins. The adhesion results provide further insights into the antibiofouling activity against barnacle cyprid settlement attributed to polysulfobetaine brushes.

show abstract

“…Many bioinert polymers have thus far been developed as a coating material that leads to the durability, efficacy, and safety of various articles such as ship bottoms and food packaging. In particular, poly(ethylene oxide) (PEO), , zwitterionic polymers, , and their derivatives , have been widely used in biorelated fields. Recently, a possible mechanism for how such polymers including poly(vinyl ether) derivatives and poly(2-methoxyethyl acrylate) − exhibit such excellent anti-biofouling properties has been proposed on the basis of the interfacial aggregation states and dynamics in water.…”

mentioning

confidence: 99%

Design of a Bioinert Interface Using an Amphiphilic Block Copolymer Containing a Bottlebrush Unit of Oligo(oxazoline)

Hong

Totani

Kawaguchi

et al. 2020

ACS Appl. Bio Mater.

View full text Add to dashboard Cite

We designed an amphiphilic block copolymer, poly(methyl methacrylate)-block-poly[oligo(2-ethyl-2-oxazoline) methacrylate] (PMMA-b-P[O(Ox)MA]), suitable for bioinert coating. Angular-dependent X-ray photoelectron spectroscopy and neutron reflectivity measurements revealed that the outermost surface of a dried film of PMMA-b-P[O(Ox)MA] was covered with the PMMA block-rich layer. Once the film came into contact with water, the P[O(Ox)MA] bottlebrush block was segregated toward the water interface. This structural rearrangement in the outermost region of the film resulted in the formation of the swollen oligo(oxazoline) layer with excellent bioinertness in terms of the suppression of serum protein adsorption and NIH3T3 fibroblast adhesion.

show abstract

Adhesion force measurement of live cypris tentacles by scanning probe microscopy in seawater

Cited by 8 publications

References 36 publications

A Facile Surface Functionalization Method for Polymers Using a Nonsolvent

A Facile Surface Functionalization Method for Polymers Using a Nonsolvent

Brush Swelling and Attachment Strength of Barnacle Adhesion Protein on Zwitterionic Polymer Films as a Function of Macromolecular Structure

Design of a Bioinert Interface Using an Amphiphilic Block Copolymer Containing a Bottlebrush Unit of Oligo(oxazoline)

Contact Info

Product

Resources

About