Adhesion force between calcium oxalate monohydrate crystal and kidney epithelial cells and possible relevance for kidney stone formation

Rabinovich, Ya. I.; Esayanur, Madhavan S; Daosukho, Saijit; Byer, Karen J.; El‐Shall, H.; Khan, Saeed R.

doi:10.1016/j.jcis.2006.03.070

Cited by 37 publications

(24 citation statements)

References 35 publications

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“…[6]. The intersection of the crystal planes 12 1, 02 1 and 010 faces of the COM crystal was used in the measurements.…”

Section: Methodsmentioning

confidence: 99%

“…All measurements were conducted in a Tris-buffer solution (TBS) of the following composition: 10 mM Tris (NH 4 C(CH 2 OH) 3 [1,6]. Both LLC-PK 1 and MDCK have a microvillous brush border and form continuous monolayers in culture with uniform cell-to-cell contacts.…”

Section: Methodsmentioning

confidence: 99%

“…The authors took a different approach [6] and measured the adhesion force between the edge of the COMcrystal mounted on the AFM tip and the layer of the kidney epithelial cells, more closely replicating what may happen inside the kidneys. The adhesion force for Madin-Darby canine kidney (MDCK) epithelial cells (a collecting duct renal epithelium line) was found to be in the range 0.25-0.5 nN, however no adhesion was found for porcine kidney (LLC-PK 1 ) epithelial cells (a proximal tubular line).…”

Section: Introductionmentioning

confidence: 99%

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Direct AFM measurements of adhesion forces between calcium oxalate monohydrate and kidney epithelial cells in the presence of Ca2+ and Mg2+ ions

Rabinovich

Daosukho

Byer

et al. 2008

Journal of Colloid and Interface Science

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“…[6]. The intersection of the crystal planes 12 1, 02 1 and 010 faces of the COM crystal was used in the measurements.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Direct AFM measurements of adhesion forces between calcium oxalate monohydrate and kidney epithelial cells in the presence of Ca2+ and Mg2+ ions

Rabinovich

Daosukho

Byer

et al. 2008

Journal of Colloid and Interface Science

Self Cite

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“…In turn, this was associated with a markedly increased deposition of CaOx stones in the kidney. Previous studies showed that renal tubular epithelial cell injury is a key factor in the stone formation process following shockwave lithotripsy [17][18][19]. Tissue injury during ultrasound lithotripsy can be due to either thermal or mechanical eVect on the urothelium with direct application [15].…”

Section: Discussionmentioning

confidence: 99%

Tissue effects of intracorporeal lithotripsy techniques during percutaneous nephrolithotomy: comparison of pneumatic and ultrasonic lithotripters on rat bladder

et al. 2011

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The objectives of this study were to determine the tissue effects of ultrasonic and pneumatic lithotripsy on the rat urothelium. The rats were divided into three groups. Groups I and II consisted of ten rats each that underwent intracorporeal lithotripsy (pneumatic and ultrasonic lithotripsy, respectively). Group III contained ten control rats and no lithotripsy method was used, they served as references for absence of injury. The light microscopy findings were evaluated as follows: squamous metaplasia, papillary projection, inflammation, increased stratification, and stone formation. In five (71.4%) animals of group II, bladders were edematous and hemorrhagic, macroscopically. Histologically, the bladder wall was normal in four rats of group I and in one of group II. There was a significant increase in inflammation (31.5%), squamous metaplasia (85.7%), papillary projection (71.4%), increased stratification (71.4%), and microscopic or macroscopic stone formation (85.7%) in the bladder wall of group II rats in comparison with group I and control group. In the rat model, we noted that ultrasonic devices have a potential risk for tissue injury. In turn, this was associated with a markedly increased deposition of CaOx stones in the kidney. When confronted with harder stones, pneumatic lithotripsy can be more effective while also minimizing tissue injury.

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“…This will allow sufficient crystal growth and aggregation for problematic stone size. Studies have confirmed crystal retention on the tubular wall of nephron and also highlighted the importance of specific anionic sites at the cell surface for the binding of crystals on tubular epithelium (Lieske et al, 1996b(Lieske et al, , 2000Asselman et al, 2003Asselman et al, , 2005Rabinovich et al, 2006). HA is a linear polysaccharide whose length can reach tenths of micrometers with a carboxyl group every two sugar rings.…”

Section: Introductionmentioning

confidence: 98%

Characterization of hyaluronic acid interaction with calcium oxalate crystals: implication of crystals faces, pH and citrate

Lamontagne

Plante

Grandbois

2011

J of Molecular Recognition

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Interaction between hyaluronic acid (HA) present at the surface of tubular epithelial cells and calcium oxalate monohydrate (COM) crystals is thought to play an important role in kidney stone formation. AFM-based force spectroscopy, where HA is covalently attached to AFM-probes, was used to quantify the interaction between HA and the surfaces of COM crystals. The work of adhesion of the HA-probe as well as the rupture force of single HA molecules were quantified in order to understand the molecular regulation of HA binding to COM crystals. Our results reveal that HA adsorbs to the crystal surface in physiological conditions. We also observed increased adhesion when the pH is lowered to a value that increases the risk of kidney stone formation. HA adhesion to the COM crystal surface can be suppressed by citrate, a physiological inhibitor of stone retention currently used in the treatment and prevention of kidney stone formation. Interestingly, we also observed preferential binding of HA onto the [100] face versus the [010] face, suggesting a major contribution of the [100] faces in the crystal retention process at the surface of tubular epithelial cells and the promotion of stone formation. Our results clearly establish a direct role for the glycosaminoglycan HA present at the surface of kidney tubular epithelium in the process of COM crystal retention.

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Adhesion force between calcium oxalate monohydrate crystal and kidney epithelial cells and possible relevance for kidney stone formation

Cited by 37 publications

References 35 publications

Direct AFM measurements of adhesion forces between calcium oxalate monohydrate and kidney epithelial cells in the presence of Ca2+ and Mg2+ ions

Direct AFM measurements of adhesion forces between calcium oxalate monohydrate and kidney epithelial cells in the presence of Ca2+ and Mg2+ ions

Tissue effects of intracorporeal lithotripsy techniques during percutaneous nephrolithotomy: comparison of pneumatic and ultrasonic lithotripters on rat bladder

Characterization of hyaluronic acid interaction with calcium oxalate crystals: implication of crystals faces, pH and citrate

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