Adhesion control of fungal spores on solid surfaces using hydrophilic nanoparticles

Nomura, Toshiyuki; Minamiura, Mana; Fukamachi, Kazuto; Yumiyama, Shohei; Kondô, Akira; Naito, Makio

doi:10.1016/j.apt.2018.01.007

Cited by 8 publications

(6 citation statements)

References 24 publications

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“…The binding of fungal conidia to surfaces is influenced by both the properties of the surface and of the fungal spores [2]. The binding of fungal spores to a surface is influenced by many factors such as surface topography, chemistry, physicochemistry and electrostatic interactions [2,[7][8][9][10]. However, the interactions at the cell:substratum interface may also be influenced by the experimental methodology.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Surface Hydrophobicity on the Attachment of Fungal Conidia to Substrates of Polyvinyl Acetate and Polyvinyl Alcohol

et al. 2020

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Biofouling of PVAc and PVOH surfaces by fungal conidia can result in surface discolouration and subsequent biodeterioration. In order to understand the interactions of fungal conidia on polymer surfaces, the surface properties of PVAc and PVOH and the hydrophobicity, size and shape of three type of fungal conidia was determined (Aspergillus niger 1957, Aspergillus niger 1988 and Aureobasidium pullulans). Fungal conidia were used in a range of binding assays (attachment, adhesion and retention). The PVAc and PVOH demonstrated different surface topographies and the PVAc demonstrated a higher maximum height (300.6 nm) when compared to the PVOH (434.2 nm). The PVAc surfaces was less wettable (75°) than the PVOH surface (62°). The FTIR demonstrated differences in the chemistries of the two surfaces, whereby the PVOH confirmed the presence of polar moieties. Hydrophobicity assays demonstrated that both A. niger species' were more non-wettable than the A. pullulans. Following the attachment assays, the more hydrophobic Aspergillus spp. conidia attached in greater numbers to the more wettable surface and the A. pullulans was retained in greater numbers to the less wettable PVAc surface. The adhesion and retention assays demonstrated that the more polar surface retained all the types of conidia, regardless of their surface hydrophobicities. This study demonstrated that conidial binding to the surfaces were influenced by the chemistry and physicochemistry of the surfaces and spores. However, the inclusion of a washing stage influenced the adhesion of conidia to surfaces. In environments that were indicative of a attachment or retention assay a PVAc surface would reduce the number of A. niger spp. spores whilst a PVOH surface would reduce the number of A. pullulans spores. However, in an environment similar to a adhesion assay, a PVAc surface would be most beneficial to reduce spore retention. Thus, the use of the correct methodology that reflects the environment in which the surface is to be used is important in order to accurately inform hygienic surface development.

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Section: Introductionmentioning

confidence: 99%

The Effect of Surface Hydrophobicity on the Attachment of Fungal Conidia to Substrates of Polyvinyl Acetate and Polyvinyl Alcohol

et al. 2020

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“…Super hydrophobic structures are often used as biomimetic anti-biofouling and self-cleaning surfaces [ 23 , 24 ]. However, previous studies looking at fungal conidia adhesion to surfaces reported contradicting results of increased adhesion to hydrophobic [ 67 , 68 ] or hydrophilic [ 69 ] surfaces, and some have demonstrated that surface wettability did not play a major role in the adhesion of conidia [ 70 ]. Our study suggests that, in the case of B. cinerea , there is no correlation between surface hydrophobicity and conidia adhesion.…”

Section: Discussionmentioning

confidence: 99%

Self-Cleaning Biomimetic Surfaces—The Effect of Microstructure and Hydrophobicity on Conidia Repellence

et al. 2022

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Modification of surface structure for the promotion of food safety and health protection is a technology of interest among many industries. With this study, we aimed specifically to develop a tenable solution for the fabrication of self-cleaning biomimetic surface structures for agricultural applications such as post-harvest packing materials and greenhouse cover screens. Phytopathogenic fungi such as Botrytiscinerea are a major concern for agricultural systems. These molds are spread by airborne conidia that contaminate surfaces and infect plants and fresh produce, causing significant losses. The research examined the adhesive role of microstructures of natural and synthetic surfaces and assessed the feasibility of structured biomimetic surfaces to easily wash off fungal conidia. Soft lithography was used to create polydimethylsiloxane (PDMS) replications of Solanum lycopersicum (tomato) and Colocasia esculenta (elephant ear) leaves. Conidia of B. cinerea were applied to natural surfaces for a washing procedure and the ratios between applied and remaining conidia were compared using microscopy imaging. The obtained results confirmed the hypothesis that the dust-repellent C. esculenta leaves have a higher conidia-repellency compared to tomato leaves which are known for their high sensitivities to phytopathogenic molds. This study found that microstructure replication does not mimic conidia repellency found in nature and that conidia repellency is affected by a mix of parameters, including microstructure and hydrophobicity. To examine the effect of hydrophobicity, the study included measurements and analyses of apparent contact angles of natural and synthetic surfaces including activated (hydrophilic) surfaces. No correlation was found between the surface apparent contact angle and conidia repellency ability, demonstrating variation in washing capability correlated to microstructure and hydrophobicity. It was also found that a microscale sub-surface (tomato trichromes) had a high conidia-repelling capability, demonstrating an important role of non-superhydrophobic microstructures.

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“…For instance, the species of Deuteromycotina, Ascomycotina along with Basidiomycotina are predominant in outdoor aerosols described by Zoppas et al in the city of Brazil. 16 Fungal spores also exhibit seasonal variations. The report by Lang et al 15 demonstrated that factors such as PM10 content NO 2 , SO 2 , and temperature influence the fungal spores population significantly in the environment.…”

Section: Important Findings Of Aerosol Originated Fungal Sporesmentioning

confidence: 99%

Environmental fungal spore aerosolization: a review

Singh¹,

Bhange²

2023

JBMOA

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Bioaerosol containing fungal spores became public health hazards. The aerosols contain the fungal spores of different species of Aspergillus, Cladosporium, Chaetomium, Penicillium, Wallemia, Stachybotrys etc. and caused various life-threatening respiratory diseases such as hypersensitivity, pneumonia, Aspergillosis, Candidiasis, Mucormycosis, Cancer, etc. They are easily transmitted from one individual to another. They also cause extreme damage to crops and create problems in food security by producing mycotoxins. The transmissions of fungal spores depend upon the environmental factor, seasonal variation, growth surface, type of fungal spore, etc. There are various biophysical, biochemical and molecular techniques that are present to detect fungal spores in aerosol. There are numerous physical and chemical agents that can kill fungi. Good public health and food security can be achieved through the detection and management of fungal spores in aerosols.

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Adhesion control of fungal spores on solid surfaces using hydrophilic nanoparticles

Cited by 8 publications

References 24 publications

The Effect of Surface Hydrophobicity on the Attachment of Fungal Conidia to Substrates of Polyvinyl Acetate and Polyvinyl Alcohol

The Effect of Surface Hydrophobicity on the Attachment of Fungal Conidia to Substrates of Polyvinyl Acetate and Polyvinyl Alcohol

Self-Cleaning Biomimetic Surfaces—The Effect of Microstructure and Hydrophobicity on Conidia Repellence

Environmental fungal spore aerosolization: a review

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