Adhesion, Autoaggregation and Hydrophobicity of Six Lactobacillus Strains

Abdulla, Anwar A.; Abed, Thikra A.; Saeed, A. Mahdi

doi:10.9734/bmrj/2014/6462

Cited by 66 publications

(50 citation statements)

References 24 publications

(37 reference statements)

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“…Similar results were obtained by Pelletier et al, (1997) and Harty, Patrikakis, and Knox (1993) concerning the L. casei cells and by Zavisic et al, (2011), Jamaly, Benjouad, andBouksaim (2011) and Abdulla, Abed, and Saeed (2014) concerning the L. plantarum cells.…”

supporting

confidence: 87%

In vitro study on the cell adhesion ability of immobilized lactobacilli on natural supports

Sidira

Kourkoutas

Charalampopoulos

2015

Food Research International

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The aim of the present study was to investigate the effect of probiotic immobilization onto wheat grains, both wet and freeze dried, on the adhesion properties of the probiotic cells and make comparisons with wet and freeze dried free cells. Lactobacillus casei ATCC 393 and Lactobacillus plantarum NCIMB 8826 were used as model probiotic strains. The results showed satisfactory adhesion ability of free cells to a monolayer of Caco-2 cells (>1000CFU/100 Caco-2 cells for wet cells). Cell immobilization resulted in a significant decrease in adhesion, for both wet and freeze dried formulations, most likely because immobilized cells did not have direct access to the Caco-2 cells, but it still remained in adequate levels (>100CFU/100 Caco-2 cells for wet cells). No clear correlation could be observed between cell adhesion and the hydrophobicity of the bacterial cells, measured by the hexadecane adhesion assay. Most notably, immobilization enhanced the monolayer integrity of Caco-2 cells, demonstrated by a more than 2-fold increase in transepithelial electrical resistance (TEER) compared to free cells. SEM micrographs ascertained the adhesion of both immobilized and free cells to the brush border microvilli. Finally, the impact of the food matrix on the adhesion properties of probiotic bacteria and on the design of novel functional products is discussed.

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supporting

confidence: 87%

In vitro study on the cell adhesion ability of immobilized lactobacilli on natural supports

Sidira

Kourkoutas

Charalampopoulos

2015

Food Research International

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“…The adherence of our Lactobacillus isolates were quantities by light microscopy after Gram staining. 5 strains of our Lactobacillus strains showed a strongly potent adhesion to cells; however, other 3strains showed weak adhesion and L. reuteri strain M20 determined as non-adherent.Overall, the capacity of adhesion for all tested strains was different; this different species adhesion is not a new phenomenon and has reported by various investigators (Abdulla et al, 2014;Granato et al, 2004 ).…”

Section: Bacteria Pathogenssupporting

confidence: 60%

Selection and characterization of potential probiotic <italic>Lactobacilli spp</italic> isolated from chicken feces may be used as a potent antibacterial agent

Aazami

Kalantar

Poormazaheri

et al. 2016

AJDFR

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Lactobacillus strains commonly used as probiotics which possess some properties such as resistance to gastric acidity and bile salts, production of antimicrobial compounds, the ability to modulate immune responses, and adhesion to gut tissues. This study aimed to isolate, identify, production and characterization of the probiotic strains. Total of 10 digestive tracts of chickens fed without antibiotics for presence of Lactobacillus spp was evaluated. All isolates were grown with different conditions like effect of temperature, pH, pepsine, bile, sodium chloride tolerance, aggregation, adhesion and antibiotic resistance test tested and then antibacterial activity was determined.In this study, 34 lactic acid bacteria were investigated as putative probiotic candidates. Based on the molecular identification using 16S rDNA sequencing the isolates belonged to Lactobacillus species. The strains showed potent antibacterial activity. The results on acid tolerance showed that all the strains tested survived at pH 2.0 to 5.5. L. agilis strain M18, L. salivarius strain M10, L.vaginalis strains M8 and M19, and L. crispatus strains Or2 and M10 showed the lowest sensitivity to pepsine. All the studied Lactobacillus strains were able to grow at both 15 and 45°C . Five Lactobacillus strains adhered well to the Caco-2 cells. The result of antibiotic resistance test of 34 lactic acid bacteria showed that most of them were resistant or intermediate. Based on 34 Lactobacillus strains, L. vaginalis strain M8 and L. reuteri strain M6 were selected for the further in vivo assays and possible eventual use as prophylactic and therapeutic agent.

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“…Physiochemical properties of the bacterial cell surface can in fact be modified depending on the physiological state of the cell, nutrient composition [29,30] and pH of the growth medium [28]. …”

Section: Discussionmentioning

confidence: 99%

In Vitro Assessment of the Probiotic Potential of Lactococcus lactis LMG 7930 against Ruminant Mastitis-Causing Pathogens

2017

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Mastitis in dairy ruminants is considered to be the most expensive disease to farmers worldwide. Recently, the intramammary infusion of lactic acid bacteria has emerged as a potential new alternative to antibiotics for preventing and treating bovine mastitis. In this study we have investigated in vitro the probiotic potential of Lactococcus lactis LMG 7930, a food-grade and nisin-producing strain, against mastitis-causing pathogens. We have characterized its carbohydrate fermentation and antibiotic susceptibility profiles, cell surface properties and antimicrobial activity, as well as its capabilities to adhere to and inhibit the invasion of pathogens into the bovine mammary epithelial cell line BME-UV1d. We found that L. lactis LMG 7930 was sensitive to tested drugs, according to the EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP), and showed an improved carbohydrate fermentation capacity compared to starter strains. Moreover, the strain exhibited antagonistic properties towards many of the pathogens tested. It presented medium surface hydrophobicity, a low basic property and no electron acceptor capability. It showed low auto-aggregation and no co-aggregation abilities towards any of the tested pathogens. The strain was one of the most adhesive to bovine mammary epithelial cells among tested bacteria, but its internalisation was low. The strain did not affect significantly pathogen invasion; however, a trend to decrease internalization of some pathogens tested was observed. In conclusion, our results suggest that this strain might be a promising candidate for the development of new strategies of mastitis control in ruminants. Future investigations are needed to evaluate its safety and efficacy under field conditions.

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Adhesion, Autoaggregation and Hydrophobicity of Six Lactobacillus Strains

Cited by 66 publications

References 24 publications

In vitro study on the cell adhesion ability of immobilized lactobacilli on natural supports

In vitro study on the cell adhesion ability of immobilized lactobacilli on natural supports

Selection and characterization of potential probiotic <italic>Lactobacilli spp</italic> isolated from chicken feces may be used as a potent antibacterial agent

In Vitro Assessment of the Probiotic Potential of Lactococcus lactis LMG 7930 against Ruminant Mastitis-Causing Pathogens

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