Adhesion and proliferation of human Schwann cells on adhesive coatings

Vleggeert-Lankamp, Carmen; Pêgo, Ana Paula; Lakke, E.A.J.F.; Deenen, M.G.M.; Marani, Enrico; Thomeer, R.T.W.M.

doi:10.1016/j.biomaterials.2003.09.067

Cited by 59 publications

(41 citation statements)

References 55 publications

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“…Cell alignment has been reported effective on chemical cues alone, such as on alternating 20 mm laminin and BSA coating (94%, Thompson and Buettner, 2001), and on 20-30 mm laminin coated stripes (50%, Schmalenberg and Uhrich, 2005). In our study, the laminin coating on grooved surfaces had a better effect in shorter term (<24 h), but not as noteworthy after 48 h. This agrees with the previous findings that laminin improved adhesion of Schwann cells onto the substrates (Miller et al, 2001a;Vleggeert-Lankamp et al, 2004), but microgrooves caused the Schwann cells to align when the laminin was adsorbed (Miller et al, 2001a). The finding that the deeper grooves lessened the impact with ECM proteins also suggests it is repulsion from the edge that causes the aligned morphology and not anything intrinsic to the groove.…”

Section: Groove Width/spacing/depthsupporting

confidence: 95%

“…In our study, the effect of laminin on Schwann cell attachment and alignment at 24 h were both superior to that of collagen type I. In spite of a similar conclusion, the effect of these two coatings on cell adhesion was unequal, disagreeing that reported by Vleggeert-Lankamp et al (2004) (Miller et al, 2001a;Schmalenberg and Uhrich, 2005) for studying alignment have been used. The seeding density selected in our study ($10 4 cells/cm 2 ) fell in the middle of such range.…”

Section: Groove Width/spacing/depthcontrasting

confidence: 81%

“…The effect of a number of different ECM coatings including laminin and collagen type I on the adhesion and proliferation of human Schwann cells has been studied recently (Vleggeert-Lankamp et al, 2004). Laminin and collagen type I on glass coverslips enhanced the adhesion of human Schwann cells equally at 24 h, but the cell proliferation after 72 h on laminin was much better than on collagen type I.…”

Section: Groove Width/spacing/depthmentioning

confidence: 99%

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Oriented Schwann cell growth on microgrooved surfaces

Hsu

Chen

et al. 2005

Biotech & Bioengineering

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Silicon wafers bearing microgrooved surfaces with various groove width, spacing, and depth were fabricated using microlithography. The orientation of rat Schwann cells along the direction of the grooves was measured at 24 h after seeding the cells. When the width/spacing of the grooves was fixed at 10/10 microm, the mean percentage of aligned cells was 12% for grooves of 0.5 microm depth, 15% for those of 1 microm depth, and 26% for those of 1.5 microm depth (P < 0.05). When the depth of grooves was fixed at 1.5 microm, the mean percentage of aligned cells increased from 26% for width/spacing 10/10 microm, to 33% for 10/20 microm or 20/10 microm, and up to 41% for 20/20 microm (P < 0.05). On the surface with grooves of width/spacing/depth = 20/20/1.5 microm and modified by laminin, the alignment at 24 h approached 60%, versus 51% for collagen-coated surface and 41% for uncoated surface (P < 0.05). At 48 h after seeding, about 66% of the cells were aligned on the above laminin-modified surface. The groove depth influenced orientation of Schwann cells significantly. The cell alignment on 20/20/3 microm microgrooved poly(D,L-lactide-co-glycolide) 90:10 (PLGA) surfaces transferred from silicon reached 72% at 48 h and 92% at 72 h (P < 0.05). Coating this surface with laminin enhanced cell alignment only in short term (67% vs. 62% at 24 h, P < 0.05). The cell alignment guided by surface microgrooves was time dependent.

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Section: Groove Width/spacing/depthsupporting

confidence: 95%

Section: Groove Width/spacing/depthcontrasting

confidence: 81%

Section: Groove Width/spacing/depthmentioning

confidence: 99%

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Oriented Schwann cell growth on microgrooved surfaces

Hsu

Chen

et al. 2005

Biotech & Bioengineering

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“…To examine whether specific activation of CD151-associated ␤ 1 integrins affects association between CD151-␤ 1 integrin complexes and small GTPases, we precoated the culture plate surface with either poly-L-lysine or laminin before seeding the cells. Compared with cell binding to poly-L-lysine, a cationic polyelectrolyte that minimally activates any types of integrins (51)(52)(53), the attachment of CD151-transfected MelJuSo cells to laminin, which strongly activates CD151-associated ␣ 3 ␤ 1 /␣ 6 ␤ 1 integrins, significantly increased the levels of small GTPases associated with CD151 and ␤ 1 integrins (Fig. 4A).…”

Section: Engagement Of ␤ 1 Integrins By Laminin Binding Augments Molementioning

confidence: 99%

Tetraspanin CD151 Stimulates Adhesion-dependent Activation of Ras, Rac, and Cdc42 by Facilitating Molecular Association between β1 Integrins and Small GTPases

Hong

Jeoung

et al. 2012

Journal of Biological Chemistry

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Background: CD151 associates with integrins and regulates integrin-dependent small GTPase-mediated cellular behaviors. Results: CD151-expressing cells exhibited increased Ras, Rac, and Cdc42 complexes with integrins and active forms of small GTPases, as compared with CD151-deficient cells. Conclusion: CD151 contributes to integrin signaling to small GTPases by facilitating association of integrins with small GTPases. Significance: Investigating integrin interactions with small GTPases is critical for understanding adhesion signaling.

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“…It is well known that cells can attach to areas where proteins such as fibronectin, collagen, etc. had absorbed [11][12][13] . Thus, we investigated the adsorption of fibronectin and albumin to films by QCM in this study.…”

Section: Discussionmentioning

confidence: 99%

Biological and Chemical Assessment of DNA/Chitosan Complex Film

Inoue¹,

Kawaguchi²,

Masui³

et al. 2014

J. Hard Tissue Biology.

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Transparent DNA/chitosan complex film was prepared from DNA/chitosan complex powder via hydrothermal hot pressing. In this study, we investigated the binding of daunorubicin hydrochloride (DH), proteins adsorption (fibronectin and albumin), mineral deposition, and rat soft-tissue response to the DNA/ chitosan complex film to determine whether this film has potential for use in membranes for bone tissue engineering. The binding ratio of DH to DNA/chitosan complex film was very close to the excision number of 3.7 for the binding of DH by native DNA. This indicated that DNA in the complex did not denature as a result of hot pressing. Apatite was formed on the surface of the film after immersion in simulated body fluid. However, quartz crystal microbalance analysis showed that protein absorption to the DNA/chitosan complex was very low. It was found that this film has an affinity for minerals rather than proteins. Although the film remained in an almost unchanged configuration at 21 days after subcutaneous implantation, the response of rat soft tissues to the film was mild. These results suggest that DNA/chitosan complex film has potential for use as a membrane in bone tissue engineering.

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Adhesion and proliferation of human Schwann cells on adhesive coatings

Cited by 59 publications

References 55 publications

Oriented Schwann cell growth on microgrooved surfaces

Oriented Schwann cell growth on microgrooved surfaces

Tetraspanin CD151 Stimulates Adhesion-dependent Activation of Ras, Rac, and Cdc42 by Facilitating Molecular Association between β1 Integrins and Small GTPases

Biological and Chemical Assessment of DNA/Chitosan Complex Film

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