Adhesion and metabolic activity of human corneal cells on PCL based nanofiber matrices

Stafiej, Piotr; Küng, Florian; Thieme, Daniel; Czugala, Marta; Kruse, Friedrich E.; Schubert, Dirk W.; Fuchsluger, Thomas Armin

doi:10.1016/j.msec.2016.10.058

Cited by 48 publications

(34 citation statements)

References 19 publications

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“…A cell culture experiment could be the first step for testing the biocompatibility of natural or modified materials 30 , 31 . Isolated corneal epithelial cells, endothelial cells, corneal keratocytes, and limbal stem cells have been used as a testing protocol in ophthalmological applications 12 . In this experiment, 3D nanofibrous PCL and PCL/collagen mats with specific nano-patterns were studied as potential matrices for ocular tissue reconstruction.…”

Section: Methodsmentioning

confidence: 99%

“…In particular, biomaterials for corneal tissue engineering must demonstrate several important functions for their potential utility in vivo , including transparency, biocompatibility and slow biodegradability 8 – 11 . A typical treatment for injured corneal tissue is an implantation of a patch of amniotic membrane in which the cornea cells or stem cells are cultured 12 . However, since it is difficult to obtain a human amniotic membrane (hAM) for therapeutic use, attention has been paid to developing an alternative carrier having the immune-privileged, anti-inflammatory, and growth-promoting properties of amniotic membrane for the ocular surface reconstruction.…”

Section: Introductionmentioning

confidence: 99%

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Fabrication of transparent hemispherical 3D nanofibrous scaffolds with radially aligned patterns via a novel electrospinning method

Kim

Park

2018

Sci Rep

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Tissue engineering has significantly contributed to the development of optimal treatments for individual injury sites based on their unique functional and histologic properties. Human organs and tissue have three-dimensional (3D) morphologies; for example, the morphology of the eye is a spherical shape. However, most conventional electrospinning equipment is only capable of fabricating a two-dimensional (2D) structured fibrous scaffold and no report is available on a 3D electrospinning method to fabricate a hemispherical scaffold to mimic the native properties of the cornea, including microscopic to macroscopic morphology and transparency. We proposed a novel electrospinning method using a single nonconductive hemispherical device and a metal pin. A designed peg-top shaped collector, a hemispherical nonconductive device with a metal pin in the center and copper wire forming a circle around at the edge was attached to a conventional conductive collector. A 3D hemispherical transparent scaffold with radially aligned nanofibers was successfully fabricated with the designed peg-top collector. In summary, our fabricated 3D electrospun scaffold is expected to be suitable for the treatment of injuries of ocular tissues owing to the hemispherical shape and radially aligned nanofibers which can guide the direction of the main collagen and cellular actin filament in the extracellular matrix.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Fabrication of transparent hemispherical 3D nanofibrous scaffolds with radially aligned patterns via a novel electrospinning method

Kim

Park

2018

Sci Rep

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“…8 Because of its high crystallinity and high hydrophobicity, it degrades at a much slower rate than PLA. 6 PCL has also been approved by the FDA for clinical applications, and is extensively used in lots of biomedical applications due to the proper tensile property and biocompatibility, 9,10,11,12 pure or in combination with other polyesters in order to modulate its degradation kinetics.…”

Section: Introductionmentioning

confidence: 99%

PLA/PCL electrospun membranes of tailored fibres diameter as drug delivery systems

Herrero-Herrero

Gómez-Tejedor

Vallés-Lluch

2018

European Polymer Journal

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The main electrospinning parameters, i.e., polymer concentration in the injectable solution, solvents used and their proportion, flow rate, voltage and distance to collector were herein systematically modified to analyse their particular influence in fibres diameter of electrospun membranes of poly(lactic acid), polycaprolactone and their mixture. As a result of this analysis, the procedures to obtain membranes of these polymers and blend with under-and above-micron-sized fibres were established, in which the solvents ratio (chloroform/methanol and dichloromethane/dimethylformamide) and voltage were found to play the major role. Moreover, the plausible differential effect of these fibres diameters (0.8 and 1.8 µm) in the controlled release of a molecule of interest was explored, using bovine serum albumin (BSA), proving that the most effective configuration for BSA release among those studied was the PLA-PCL combination in membranes of above-micron fibres diameter.

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“…This is mainly due to its tunable degradation profile, in combination with low cost, easy production and availability of medical-grade material [ 3 , 4 ]. It is thus not surprising that PCL nanofibers for biomedical applications have also been developed [ 5 , 6 , 7 , 8 , 9 , 10 ]. The practical application of PCL nanofibers in the biomedical field, however, is severely limited due to the hydrophobic properties adversely affecting cell affinity.…”

Section: Introductionmentioning

confidence: 99%

Nanostructured Hydrogels by Blend Electrospinning of Polycaprolactone/Gelatin Nanofibers

et al. 2018

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Nanofibrous membranes based on polycaprolactone (PCL) have a large potential for use in biomedical applications but are limited by the hydrophobicity of PCL. Blend electrospinning of PCL with other biomedical suited materials, such as gelatin (Gt) allows for the design of better and new materials. This study investigates the possibility of blend electrospinning PCL/Gt nanofibrous membranes which can be used to design a range of novel materials better suited for biomedical applications. The electrospinnability and stability of PCL/Gt blend nanofibers from a non-toxic acid solvent system are investigated. The solvent system developed in this work allows good electrospinnable emulsions for the whole PCL/Gt composition range. Uniform bead-free nanofibers can easily be produced, and the resulting fiber diameter can be tuned by altering the total polymer concentration. Addition of small amounts of water stabilizes the electrospinning emulsions, allowing the electrospinning of large and homogeneous nanofibrous structures over a prolonged period. The resulting blend nanofibrous membranes are analyzed for their composition, morphology, and homogeneity. Cold-gelling experiments on these novel membranes show the possibility of obtaining water-stable PCL/Gt nanofibrous membranes, as well as nanostructured hydrogels reinforced with nanofibers. Both material classes provide a high potential for designing new material applications.

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Adhesion and metabolic activity of human corneal cells on PCL based nanofiber matrices

Cited by 48 publications

References 19 publications

Fabrication of transparent hemispherical 3D nanofibrous scaffolds with radially aligned patterns via a novel electrospinning method

Fabrication of transparent hemispherical 3D nanofibrous scaffolds with radially aligned patterns via a novel electrospinning method

PLA/PCL electrospun membranes of tailored fibres diameter as drug delivery systems

Nanostructured Hydrogels by Blend Electrospinning of Polycaprolactone/Gelatin Nanofibers

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