Adherence to stainless steel by foodborne microorganisms during growth in model food systems

Hood, Scott; Zottola, E. A.

doi:10.1016/s0168-1605(97)00071-8

Cited by 240 publications

(175 citation statements)

References 24 publications

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“…A number of studies have shown that Salmonella spp are capable of adhering and forming biofilms on diverse surfaces including metal, glass and rubber surfaces (Hood and Zottola, 1997;Joseph et al, 2001;Stepanovic´et al, 2004). The assessment of biofilm formation by Salmonella on microtitre plate showed that all Salmonella isolates were able to form biofilms.…”

Section: Resultsmentioning

confidence: 99%

Biofilm formation of <i>Salmonella</i> species isolated from fresh cabbage and spinach

Isoken¹

2015

Journal of Applied Sciences and Environmental Management

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ABSTRACT:The aim of the study was to isolate Salmonella from fresh cabbage and spinach vegetables, determine antimicrobial resistance and biofilm formation of the isolates. Spinach and cabbage farm vegetables were found to harbour Salmonella. A total of eighty-two Salmonella isolates were recovered from both vegetables and subjected to antimicrobial reactions. Salmonella isolate showed sensitivity against the aminoglycoside and quinolones.Isolates from cabbage showed ≥ 80% susceptibility to nalidixic acid and ciprofloxacin and an average of 72% susceptibility was exhibited against gentamicin and ofloxacin. Also, isolates from spinach vegetable demonstrated excellent sensitivity against chloramphenicol (94%), nalidixic acid (90%) and ofloxacin (82%). Variable resistant patterns was observed for tetracycline (58%: 47%), ampicillin (55.5%: 31.4%), erythromycin (58.1%: 62.7%), streptomycin (64.5%: 76.5%), cephalothin (35.5%: 39.2%) against isolates from cabbage and spinach respectively. The assessment of biofilm formation by Salmonella on microtitre plate showed that all Salmonella isolates were able to form biofilms. Isolates from cabbage were mainly strong producer 15(48.3%), while 11(35.5%) of the isolates were moderate producers and 6(16.1%) weak producer. On the other hand, 28(54.9%) of Salmonella isolates from spinach vegetable were moderate producer, 12(23.5%) weak producer and 11(21.5%) strong producer. The finding of this study shows that cabbage and spinach is potential host for the transmission of Salmonella to humans or other animals. The ability of the isolates to form biofilm reveals the potential of the isolates to persist on the vegetable and the pathogenic status of the isolates as well as ability to resist antimicrobial chemotherapy. © JASEM http://dx

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Section: Resultsmentioning

confidence: 99%

Biofilm formation of <i>Salmonella</i> species isolated from fresh cabbage and spinach

Isoken¹

2015

Journal of Applied Sciences and Environmental Management

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“…Two publications [11,17] reported that L. monocytogenes sticks to the surface of stainless steel with vast amounts of polymeric materials. Hood and Zottola [12] showed that a level of about 10 3 L. monocytogenes cells/cm 2 adhered to the surface of stainless steel chips in reconstituted skim milk at 23°C for 1 hr. As the tank of farm A was not thoroughly cleaned with detergent, L. monocytogenes on the surface was probably protected by a layer of milk fat, protein or saccharide.…”

mentioning

confidence: 99%

Sources and Routes of Contamination of Raw Milk with Listeria monocytogenes and Its Control.

Yoshida

Kato

Sato

et al. 1998

The Journal of Veterinary Medical Science

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ABSTRACT. To identify the source of contamination of raw bulk milk with Listeria, we attempted to isolate the bacteria from various samples such as cattle-related samples, bulk storage tanks and the environments on three farms. On farms A and B, Listeria monocytogenes was repeatedly isolated from raw milk, while on farm C, it was scarcely isolated from it. On the former farms, Listeria was detected in cattle-related samples and the environments. On the other hand, only one fecal sample on the latter farm was Listeria-positive. Especially, we demonstrated that the bulk tank on farm A was contaminated with L. monocytogenes. Then, L. monocytogenes was controlled by continuously washing the bulk tank on farm A with alkaline detergent. -KEY WORDS : contamination route and source, Listeria monocytogenes, raw milk.

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“…The obtained results correspond to the reports of other researchers, indicating that a nutrient-poor medium enhances the biofilm formation of Salmonella spp. (hOOD and ZoTToLA, 1997;STEPANoVIć et al, 2004). This can be explained by the fact that the maximum expression of the major transcriptional regulator CsgD (curli subunit gene D, also described as agfD) under aerobic conditions occurs in a nutrient-limited medium (GERSTEL and RöMLING, 2001;STEPANoVIć et al, 2004).…”

Section: Congo Red Agar Test All S Tennessee Isolates (mentioning

confidence: 99%

Biofilm forming ability of Salmonella enterica serovar Tennessee isolates originating from feed

Milanov¹,

Prunić²,

Ljubojević³

2017

Vet. arhiv

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Salmonella enterica subspecies enterica serovar tennessee was the most frequent Salmonella serovar isolated from plant-based animal feed throughout a two-year period (2012)(2013) of research conducted in the region of the Autonomous Province of Vojvodina. In this study, the ability of biofilm formation was investigated on 20 isolates of S. Tennessee. The starting point for this research was the established biofilm-forming ability of serovar Tennessee strains, which significantly contributes to their wide distribution in the environment (plant materials), and hence in animal feed. In this study, biofilm formation was investigated on polystyrene (microplate biofilm assay) and stainless steel (scanning electron microscopy) surfaces by culturing in Luria Bertani broth (LB) and Triptone Soya Broth (TSB). The expression of major matrix components of Salmonella biofilm (curli fimbriae and cellulose) was examined by cultivation on Congo Red agar. All isolates of S. tennessee produced a biofilm on a polystyrene surface in a microtiter plate test, by cultivation in LB at an incubation temperature of 20 °C. Scanning electron microscopy revealed biofilm formation of S. tennessee on a stainless steel surface by cultivation in LB, but not in TSB. The expression of curli fimbriae and cellulose, as well as the formation of characteristic "rdar" colonies on Congo red agar has been observed in all isolates of S. tennessee. this serovar is not specifically adapted to humans and animals, so the available epidemiological data do not indicate its major implication in food-borne infection outbreaks. however, this serovar manifests a pronounced ability for adherence to plant surfaces, biofilm formation and increased resistance to long-term desiccation, which is the most likely explanation for its frequent identification in low water activity feed.

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Adherence to stainless steel by foodborne microorganisms during growth in model food systems

Cited by 240 publications

References 24 publications

Biofilm formation of <i>Salmonella</i> species isolated from fresh cabbage and spinach

Biofilm formation of <i>Salmonella</i> species isolated from fresh cabbage and spinach

Sources and Routes of Contamination of Raw Milk with Listeria monocytogenes and Its Control.

Biofilm forming ability of Salmonella enterica serovar Tennessee isolates originating from feed

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