Adherence of ruminant mastitis Staphylococcus aureus strains to epithelial cells from ovine mammary gland primary cultures and from a rat intestinal cell line

Iturrlde, M.; Aguilar, Begoña; Baselga, R.; Amorena, B.

doi:10.1016/0378-1135(93)90079-m

Cited by 19 publications

(14 citation statements)

References 17 publications

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“…Cultures were carried out in the 20 animals under study as previously described [5,13] and were examined daily for the development of a cytopathic effect (CPE) over a one month period. Detection of CPE was confirmed by Giemsa staining and viral proteins were detected by immunocytochemistry (ICC) using anti-p25 antibody (supplied by G.D. Harkiss, University of Edinburgh, United Kingdom).…”

Section: Cultures Of Ovine Mammary Epithelial Cellsmentioning

confidence: 99%

“…Pure MVV-free OMEC were obtained from the three control animals as previously described [5,13]. These cultures were 100% positive for cytokeratin by ICC, negative to CPE after at least 3 weeks and negative to RT-PCR for MVV, using primers for pol and gag regions of the EV1 strain of MVV and sheep Na, K-ATPase primers as internal control for RT-PCR amplification [47].…”

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confidence: 99%

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Maedi-visna virus infection of ovine mammary epithelial cells

et al. 2006

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-The aim of this work was to perform a complete study of maedi-visna virus (MVV) infected mammary glands of naturally-infected sheep, and to determine if cells other than macrophages undergo a productive viral infection in this organ. Fifteen seropositive and two seronegative ewes were selected from MVV-infected flocks on the basis of clinical indurative mastitis and three sheep from an MVV-free flock. Within the mammary gland, MVV-positive cells were located by immunohistochemistry in the stroma and the epithelial alveolar barrier, most likely the ovine mammary epithelial cells (OMEC) of the acini. In situ hybridization confirmed these findings. Ultrastructural studies showed the presence of lentivirus-like particles budding off the cell surface in the alveolar barrier and also free in the acinar lumen. The presence of mammary histopathological lesions and MVV together with clear indications of productive infection (demonstration of a cytopathic effect in OMEC cultures and infection of co-cultures) were observed in the 15 seropositive and one of the seronegative sheep from the infected flock. These findings demonstrate that the OMEC were infected in vivo and probably underwent productive infection when studied ex-vivo. The OMEC of MVV-free sheep, which had subsequently been infected in vitro with MVV, also showed productive infection when challenged in vitro, confirming the replication of MVV in OMEC in vitro. The presence of MVV-infected OMEC in the mammary gland from infected animals, the productive infection in these OMEC and the release of lentiviral particles to the acinar lumen may have relevance in the pathogenesis and transmission of MVV infection. maedi-visna / mammary gland / epithelial cells / indurative mastitis / ovine

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Section: Cultures Of Ovine Mammary Epithelial Cellsmentioning

confidence: 99%

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confidence: 99%

Maedi-visna virus infection of ovine mammary epithelial cells

et al. 2006

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“…These all promote adherence. Differences between bacterial species and strains with regard to exopolysaccharide production have been reported [1,17].…”

Section: Introductionmentioning

confidence: 99%

“…Staphylococcus aureus is the most frequent pathogen associated with metal surfaces [8], acute and chronic osteomyelitis [19]. The increasing difficulty of eliminating chronic S. aureus infections may be partly attributed to the fact that microorganisms which produce exopolysaccharides (slime) may have a special capacity to form large microcolonies and adhere to different surfaces [8,14,17]. These films are less affected by antibiotics and, because of their size, they cannot be eliminated by phagocytes [3,6].…”

Section: Introductionmentioning

confidence: 99%

Adherence of Staphylococcus aureus slime-producing strain variants to biomaterials used in orthopaedic surgery

Gracia¹,

Fernández

Conchello

et al. 1997

International Orthopaedics

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“…In this context and using highly adherent strains, we have observed that S. aureus in vitro adherence and biofilm formation on inert or mammalian cell surfaces is associated with (i) exopolysaccharide production (6,23); (ii) rough colony morphology phenotype in Congo red agar (CRA) (6); (iii) higher resistance to phagocytosis (32); (iv) lower susceptibility to antibiotics when forming biofilms (2); (v) higher capacity to attach to different surfaces and biomaterials used in orthopedic surgery, causing osteomyelitis (16); and (vi) higher capacity to colonize the ovine mammary gland, causing mastitis (6). In addition, active immunizations with exopolysaccharides extracted from a highly adherent S. aureus isolate have been shown to trigger protective immunity against mastitis (3).…”

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Bap, a Staphylococcus aureus Surface Protein Involved in Biofilm Formation

et al. 2001

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Identification of new genes involved in biofilm formation is needed to understand the molecular basis of strain variation and the pathogenic mechanisms implicated in chronic staphylococcal infections. A biofilmproducing Staphylococcus aureus isolate was used to generate biofilm-negative transposon (Tn917) insertion mutants. Two mutants were found with a significant decrease in attachment to inert surfaces (early adherence), intercellular adhesion, and biofilm formation. The transposon was inserted at the same locus in both mutants. This locus (bap [for biofilm associated protein]) encodes a novel cell wall associated protein of 2,276 amino acids (Bap), which shows global organizational similarities to surface proteins of gram-negative (Pseudomonas aeruginosa and Salmonella enterica serovar Typhi) and gram-positive (Enteroccocus faecalis) microorganisms. Bap's core region represents 52% of the protein and consists of 13 successive nearly identical repeats, each containing 86 amino acids. bap was present in a small fraction of bovine mastitis isolates (5% of the 350 S. aureus isolates tested), but it was absent from the 75 clinical human S. aureus isolates analyzed. All staphylococcal isolates harboring bap were highly adherent and strong biofilm producers. In a mouse infection model bap was involved in pathogenesis, causing a persistent infection.

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Adherence of ruminant mastitis Staphylococcus aureus strains to epithelial cells from ovine mammary gland primary cultures and from a rat intestinal cell line

Cited by 19 publications

References 17 publications

Maedi-visna virus infection of ovine mammary epithelial cells

Maedi-visna virus infection of ovine mammary epithelial cells

Adherence of Staphylococcus aureus slime-producing strain variants to biomaterials used in orthopaedic surgery

Bap, a Staphylococcus aureus Surface Protein Involved in Biofilm Formation

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