Adherence of probiotic bacteria to human colon epithelial cells and inhibitory effect against enteric pathogens – In vitro study

Abstract

The aim of this study was to determine the anti‐adherence properties of three probiotic lactobacillus strains (Lb. rhamnosus 0900, Lb. rhamnosus 0908 and Lb. casei 0919), and their mixture against pathogens: Escherichia coli ATCC 10536, Salmonella enterica serovar Typhimurium ATCC 14028 and Candida albicans ATCC 10231 using Caco‐2 human colon adenocarcinoma cells. All strains of lactobacilli and the probiotic mixture to the greatest extent inhibited adherence of S. Typhimurium, up to 91%. Lb. rhamnosus 0900 inhibited E. coli by 75.9%, and Lb. casei 0919 decreased adherence of C. albicans by 49%. All pathogens activated the adherence of the mixture of probiotic bacteria.

Comparative genomics and functional analysis of a highly adhesive dairy Lactobacillus paracasei subsp. paracasei IBB3423 strain

“…For adherence tests to epithelial tissue, the colorectal carcinoma cell line Caco-2 (Cell Line Service GmbH, Germany) from 42nd passage was cultured in T75 Roux bottles (Greiner Bio-One GmbH, Germany) as described previously (Nowak et al 2016) in DMEM (Dulbecco’s Modified Eagle’s Medium; Sigma-Aldrich, USA) supplemented with 10% FBS (fetal bovine serum; Thermo Fisher Scientific, USA), 25 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; Sigma-Aldrich, USA), 4 mM GlutaMAX (Thermo Fisher Scientific, USA), a mixture of 100 μg/ml streptomycin, and 100 IU/ml penicillin (Sigma-Aldrich, USA). Cells were incubated (37 °C, 5% CO 2 , humidity > 95%) for 7 days to reach confluence and then were detached with Tryple TM Express (Thermo Fisher Scientific, USA) according to manufacturer’s instruction.…”

Protective Activity of Probiotic Bacteria Against Candida albicans: An In Vitro Study

“…Probiotics are believed to improve the health status by decreasing pH, stimulating defense mechanism in epithelial cells, and preventing pathogen attachment. 1 A critical step in the pathogenesis and development of candidiasis is yeast adhesion to the epithelial cells. 1,15 Candida strains are able to adhere to different cell types, such as oral epithelial cells and cultured epithelial cell lines.…”

“…1 A critical step in the pathogenesis and development of candidiasis is yeast adhesion to the epithelial cells. 1,15 Candida strains are able to adhere to different cell types, such as oral epithelial cells and cultured epithelial cell lines. 11,14,16 In the present study, about 60% of C. albicans could adhere to the Hep2 epithelial cell lines.…”

“…Hence, the study of fungi is a research priority since they are eukaryotic organisms and share many biological processes with humans. [1][2][3] Among these fungi, Candida species are frequently found in the human microflora and are capable of colonizing in the oral, intestinal, and vaginal mucosa, as well as the skin.…”

Statistical optimisation of the exopolysaccharide production by Lactobacillus fermentum Lf2 and analysis of its chemical composition

“…Lactic acid bacteria (LAB) are generally recognised as safe (GRAS) microorganisms that are widely used in the food industry. Apart from their technological properties, they have been associated with several functional effects such as the inhibition of pathogens (Adriana et al ; De Sant’Anna et al ) and immunomodulatory properties (Pérez‐Cano et al ; Gao et al ). Besides, they were successfully included in several dairy matrices as well (Cordeiro et al ; Zhang et al ).…”