Adherence of non-enteropathogenic Escherichia coli to HeLa cells

Bouzari, Saeid; Jafari, Ameneh; Farhoudi-Moghaddam, A. A.; Shokouhi, F.; Parsi, Mostafa

doi:10.1099/00222615-40-2-95

Cited by 30 publications

(26 citation statements)

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“…The association of certain serogroups of EPEC with endemic infantile diarrhoea has also been reported from several countries, including Bangladesh (6), Saudi Arabia (11), Brazil (25), Uruguay (2), and in several studies (3, 23). Results of a study in Iran showed that 65% of isolates identified by commercial antisera were probably a putative EPEC (26), and although O serogrouping is not a perfect tool, in our setting it is still a useful method for presumptive identification of these strains.…”

Section: Discussionmentioning

confidence: 73%

R2205 Prevalence of enteropathogenic and shiga toxin-producing Escherichia coli among children with and without diarrhoea in Iran

Alikhani¹,

Aslani²,

Mirsalehian³

et al. 2007

International Journal of Antimicrobial Agents

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Section: Discussionmentioning

confidence: 73%

R2205 Prevalence of enteropathogenic and shiga toxin-producing Escherichia coli among children with and without diarrhoea in Iran

Alikhani¹,

Aslani²,

Mirsalehian³

et al. 2007

International Journal of Antimicrobial Agents

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“…Although LA/AA co-expression in some human E. coli has been previously reported by Bouzari et al (6), further information on these isolates is lacking. Moreover, since the expression of LA and AA is used to classify fecal E. coli as typical EPEC and EAEC (1,3), respectively, the classification of such strains within the DEC pathotypes is difficult.…”

Section: Escherichia Colimentioning

confidence: 92%

“…TOSV has also been isolated from the brain of a bat in areas where P. perniciosus and P. perfiliewi were present, but no hemagglutinationinhibiting antibodies were found in sera from these bats (5). In Spain, 2 isolates of TOSV were recovered from 103 pools of sandflies; sequence analyses showed that they were genetically divergent from the Italian strains (6). To date, TOSV had not been isolated from sandflies collected in France.…”

Section: Escherichia Colimentioning

confidence: 99%

ClassifyingEscherichia coli

Girão¹,

Girao²,

Irino³

et al. 2006

Emerg. Infect. Dis.

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“…10 DNA was extracted by means of the alkaline lysis method and dotted to nitrocellulose. 11 Hybridization was performed under stringent conditions by the digoxigenin labeling and detection system (Boehringer Mannheim, Indianapolis, IN).…”

mentioning

confidence: 99%

Short report: characterization of enteroaggregative Escherichia coli isolates from Iranian children.

Bouzari

Jafari²,

Azizi³

et al. 2001

The American Journal of Tropical Medicine and Hygiene

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Abstract.We have previously shown that enteroaggregative Escherichia coli (EAEC) is an important pathogen among Iranian infants and children. To better understand the characteristics of EAEC in Iran, we analyzed EAEC isolates for the presence of pAA plasmid-borne factors. Ninety-eight E. coli strains that displayed the aggregative adherence (AA) pattern on HeLa cells were hybridized with the CVD432 (AA) probe and with genes encoding enteroaggregative heat-stable enterotoxin-1 and aggregative adherence fimbriae (AAF) I and II. Our data suggest that AAF/II is common in this population and that AAF/I and AAF/II can sometimes be detected in the same E. coli isolate. Surprisingly, we have found that AA probe-negative strains in Iran share virulence factors with AA probepositive isolates and therefore may be more similar to probe-positive strains than previously believed. Enteroaggregative Escherichia coli (EAEC) has been associated with persistent and acute diarrhea in developing countries and in Europe. [1][2][3] Most EAEC strains harbor a 60 to 65 MDa virulence plasmid (pAA), from which Baudry and others 4 derived empirically a DNA fragment probe (called the aggregative adherence [AA] or CVD432 probe); the probe has been widely used for epidemiologic studies. 3,5,6 The pAA plasmid also encodes aggregative adherence fimbriae (AAF) I and II 7,8 and the toxin enteroaggregative heatstable enterotoxin-1 (EAST-1). 9 Notably, neither the AA probe nor any specific virulence factor is found in all EAEC strains, suggesting that EAEC strains may be highly heterogeneous.To assess the genetic characteristics of EAEC from Iranian children, we studied strains isolated from a previously described study. 10 DNA was extracted by means of the alkaline lysis method and dotted to nitrocellulose. 11 Hybridization was performed under stringent conditions by the digoxigenin labeling and detection system (Boehringer Mannheim, Indianapolis, IN). DNA probes included the 0.7-kb EcoRI/PstI AA probe 4 ; the 125-bp KpnI/XmnI fragment of pSS125 (EAST-1 12 ); the 350-bp EcoRI/PstI fragment from pJPN39 (for AAF/I 13 ); and a 1.7-kb EcoRI fragment from clone pJC2 (for AAF/II 8 ). HeLa adherence was tested by the method described by Nataro and others. 14 Of the 98 strains exhibiting the aggregative HeLa cell adherence pattern, 46 (46.9%) reacted with the AA probe. Hybridization with the EAST-1 probe revealed that only 38 (38.8%) of EAEC isolates harbored the gene encoding EAST-1; 23 (60.5%) of EAST-1-positive strains were AA probe-positive and 15 (39.5%) were AA probe-negative (P Յ 0.05). It has been shown that EAST-1 is not restricted to EAEC isolates 15 ; nonetheless, our data suggest a difference between probe-positive and probe-negative strains with regard to the frequency of this gene (Table 1).AAF/I fimbrial genes were detected in 8 (8%) of the EAEC strains; all of these were AA probe positive (for a prevalence of 17% within the probe-positive group). AAF/ II, in contrast, was detected in 25% of all strains, comprising 41% of AA probe-positive...

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Adherence of non-enteropathogenic Escherichia coli to HeLa cells

Cited by 30 publications

References 0 publications

R2205 Prevalence of enteropathogenic and shiga toxin-producing Escherichia coli among children with and without diarrhoea in Iran

R2205 Prevalence of enteropathogenic and shiga toxin-producing Escherichia coli among children with and without diarrhoea in Iran

ClassifyingEscherichia coli

Short report: characterization of enteroaggregative Escherichia coli isolates from Iranian children.

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