Adherence of <i>Yersinia enterocolitica</i> to Mammalian Epithelial Cell Lines

Okamoto, Keinosuke; Inoue, Takashi; Ichikawa, Hidetaka; Kawamoto, Yoko; Hara, Shotaro; Miyama, Akio

doi:10.1111/j.1348-0421.1980.tb02907.x

Cited by 15 publications

(12 citation statements)

References 19 publications

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“…Measurement of the association of bacteria with animal cells in vitro has most commonly been made by microscopy of stained monolayers (Hartley et al, 1978;Hackney et al, 1980;Tavendale et al, 1983). Some procedures have divided the bacterial-animal cell interaction into two phases, the infection phase and the intracellular growth phase (Lee et al, 1977;Une, 1977 ;Une et al, 1977 ;Pedersen et al, 1979 ;Okamoto et al, 1980;Soemitro et al, 1981), a consideration that appears unnecessary with Y. enterocolitica because it does not multiply intracellularly (Devenish and Schiemann,198 1). Bacteria that do not associate with the animal cells during the interaction period are usually removed by repeated washing (Maki et al, 1978;Portnoy et al, 1981;Mintz et al, 1983) but density-gradient Received 10 Jul.…”

Section: Introductionmentioning

confidence: 99%

Surface properties of Yersinia species and epithelial cell interactions in vitro by a method measuring total associated, attached and intracellular bacteria

Schiemann

Crane²,

Swanz³

1987

Journal of Medical Microbiology

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Summary.A procedure was developed for enumeration of total associated, attached and intracellular bacteria after interaction of Yersinia spp. with epithelial cells in vitro. Isogenic cultures of Y. enterocolitica grown at 25°C had greater affinity for epithelial cells (Henle, HeLa and Vero) than for polystyrene, and they invaded the cells. Y. kristenseni and Y. intermedia showed less attachment to either surface and were non-invasive. The degree of attachment to cells and invasion by Y . enterocolitica was related to number of bacteria added and interaction time, whereas attachment to polystyrene occurred rapidly and did not change. Y. enterocolitica was more hydrophobic when grown at 35°C than at 25°C according to partitioning in a biphasic dextran-polyethylene glycol system, and attached strongly to both polystyrene and epithelial-cell monolayers. Y. kristenseni grown at 25°C was also hydrophobic but did not have the same attachment properties. Y. kristenseni and Y . intermedia showed slightly reduced electrostatic interactions with the anion exchangers DEAE-Sepharose and DEAE-Trisacryl. Attachment of Y. enterocolitica to epithelial cells probably involves non-specific surface properties that are not entirely explicable by hydrophobic and electrostatic interactions, whereas invasion of epithelial cells appears to resemble "receptor-mediated endocytosis".

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Section: Introductionmentioning

confidence: 99%

Surface properties of Yersinia species and epithelial cell interactions in vitro by a method measuring total associated, attached and intracellular bacteria

Schiemann

Crane²,

Swanz³

1987

Journal of Medical Microbiology

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“…enterocolitica at 35 -37°C has also been previously reported (Lee et al 1979;Okamoto et al 1980). Yersinia enterocolitica demonstrates many other temperature-dependent characteristics, including the synthesis of certain outer membrane proteins (Portnoy et al 1981;Portnoy et al 1984).…”

Section: Discussionmentioning

confidence: 52%

“…Recent studies using tissue culture monolayers have shown that association with Vero cells (ATCC CCL-81) was greater than with HeLa or Henle cells (unpublished data). Okamoto et al (1980) reported that Y . enterocolitica adhered equally well to HeLa, FL, Y-1 adrenal, Henle, and conjunctival cells.…”

Section: Discussionmentioning

confidence: 97%

“…, including enterocolitica (Devenish and schiemann at -20°C. Subcultures were made onto trypticase soy -0.6% yeast extract (TSY) agar, incubated at room temperature for 48 h. A suspen-1981; Kapperud 19' O; Lee et 1977;Maki et 1978; sion was prepared in saline for inoculation of a defined medium with Okamoto et al 1980;Old and Robertson 1981;Pedersen et al 104-bacteria/mL, which was incubated at 250C on a roller appa-1979; Schiemann and Devenish 1982;Soemitro et al 1981; ratus for 48 h, ~h~ defined medium N a 2~p 0 4 , 0,09 M ; Une 1977;Une et al 1977 (Lee et al 1977;Okamoto et al 1980). The work (average 245 bacteria per dpm, 1 dpm = 1 Bq).…”

Section: Introductionmentioning

confidence: 99%

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Some conditions influencing the association of Yersinia enterocolitica with epithelial cells in vitro

Schiemann¹,

Swanz²

1985

Can. J. Microbiol.

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The association of Yersinia enterocolitica serotype 0:5,27 with Henle 407 epithelial cells in vitro was measured by using 35S-labelled bacteria with separation of unassociated bacteria by filtration (Nuclepore polycarbonate 5-micron membrane). The number of associated bacteria was related to the initial multiplicity. Changes in beginning pH, the presence of protein, availability of Ca2+ and Mg2+, and nature of carbohydrate in a defined bacterial growth medium did not change the degree of epithelial cell association. Bacteria recovered from the log phase of growth at 25 degrees C, or after growth to stationary phase at 35 degrees C, showed no association with epithelial cells. Optimal association occurred when the pH provided during interaction was between 7.6 and 8.6 and the temperature was either 25 or 35 degrees C. No association occurred within 30 min at 4 degrees C. The presence of Ca2+ and (or) Mg2+ during interaction had no effect, but the addition of peptone increased association. The results of this study demonstrate the importance of controlling both conditions provided for bacterial growth and those provided for interaction to achieve optimal association of Y. enterocolitica with epithelial cells in vitro.

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“…Some Y . enterocolitica adhere to epithelial cells when grown at 25°C but not at 37°C (Okamoto et al, 1980;Old and Robertson, 1981) and some adhere equally well after cultivation at 25°C or at 37°C (Lee et al, 1981 ;Old and Robertson, 1981). Because the VWderivatives showed adhesive ability with epithelial cells (Portnoy et al, 1981;Schiemann, 1981;Heesemann et al, 1983), the plasmid had not been considered to be involved in the adherence of pathogenic Y. enterocolitica.…”

Section: Discussionmentioning

confidence: 99%

Lack of correlation between haemagglutination and adherence to epithelial cells in Yersinia pseudotuberculosis

Kawaoka

Mitani²,

Hoshina³

et al. 1988

Journal of Medical Microbiology

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Summary. Yersinia pseudotuberculosis was examined for its haemagglutinating activity and adherence to cultured epithelial cells (HEp-2) in relation to possession of a virulence (VW) plasmid and to growth conditions. VW-lacking (VW -) bacteria were isolated from ten VW' strains of each serovar which, after they were grown on CFA plates at 37"C, agglutinated the erythrocytes from five different species. In contrast to the bacteria possessing the plasmid (VW') half of the VW-bacteria, grown on CFA plates at 37"C, did not agglutinate any of the erythrocytes used and the other half agglutinated only human erythrocytes. Furthermore, when grown on CFA plates at 25"C, neither VW' nor VW-bacteria showed a haemagglutinating activity. When the bacteria were grown in CFA broth, only two strains grown at 25°C did not agglutinate any of the erythrocytes tested. The VW+ and VW-bacteria of the remaining strains, grown either at 25°C or 37"C, showed relatively high haemagglutinating activity. Adherence to HEp-2 cells did not correlate with haemagglutinating activity in Y . pseudotuberculosis; the VW + bacteria grown at 37°C adhered to HEp-2 cells more efficiently than either the VW -derivatives or the VW ' bacteria grown at 25"C, regardless of the growth medium. These results indicate that some of the haemagglutinins detected on Y. pseudotuberculosis are not involved in the adherence to HEp-2 cells.

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Adherence of Yersinia enterocolitica to Mammalian Epithelial Cell Lines

Cited by 15 publications

References 19 publications

Surface properties of Yersinia species and epithelial cell interactions in vitro by a method measuring total associated, attached and intracellular bacteria

Surface properties of Yersinia species and epithelial cell interactions in vitro by a method measuring total associated, attached and intracellular bacteria

Some conditions influencing the association of Yersinia enterocolitica with epithelial cells in vitro

Lack of correlation between haemagglutination and adherence to epithelial cells in Yersinia pseudotuberculosis

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