Adherence of<i>Candida albicans</i>to host cells

Pendrak, Michael L.; Klotz, Stephen A.

doi:10.1111/j.1574-6968.1995.tb07566.x

Cited by 31 publications

(5 citation statements)

References 48 publications

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“…with comparative adherence to standard strain of Candida albicans. [26][27][28][29][30] In the present study all the oral Candida isolates (100%) showed adherence to buccal epithelial cells, which is in accordance with the study of Arati et al 31 Adherence of Candida species to BEC from cases of oral candidiasis was significant when compared with a standard strain Candida albicans MTCC 227.…”

Section: Discussionsupporting

confidence: 91%

An in vitro study on comparative adherence of oral Candida albicans and non–albicans Candida to human buccal epithelial cells and its antifungal susceptibility pattern

P. A.,

Kumar K. S.,

Kuruvilla

et al. 2023

Int J Community Med Public Health

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Background: Candida spp. are intra-oral commensals in 40-60% of human subjects and the precise factors involved in the development of Candidiasis are still not clear. The adherence of Candida to epithelial cells is one of the main pathogenic characteristics of the genus. Studies have shown that there are variations in the adherence capabilities of different Candida spp., which may explain why some species colonize mucosal surfaces more frequently than others. The aim of this study was to estimate the adherence properties of Candida spp. to buccal epithelial cells and to determine the antifungal susceptibility. Methods: 87 isolates of Candida spp. from known non-diabetic patients with oral Candidiasis were collected from diagnostic microbiology laboratories in central Kerala. The method of Kimura and Pearsall et al was followed with minor modifications to test the adherence. Results: All the three species exhibited adherence with varying capacity. C. albicans was significantly more adherent to buccal epithelial cells than C. tropicalis and C. krusei. Resistance was not observed among the isolates against fluconazole, voriconazole and clotrimazole. Conclusions: In conclusion Candida spp. isolated from oral candidiasis patients exhibited a higher adherence capacity to normal human buccal epithelial cells considered an essential virulence property. As anticipated C. albicans displayed higher virulence activity than non-Candida albicans species.

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Section: Discussionsupporting

confidence: 91%

An in vitro study on comparative adherence of oral Candida albicans and non–albicans Candida to human buccal epithelial cells and its antifungal susceptibility pattern

P. A.,

Kumar K. S.,

Kuruvilla

et al. 2023

Int J Community Med Public Health

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“…The initial observation by Klotz et al of increased aggregation as the cells and beads are mixed led to the discovery of force-induced surface ␤-aggregates in AFM experiments (Fig. 4 to 7) (63,71,89,90). Other force-generating regimens also give similar results.…”

Section: Force Initiates ␤-Aggregation and Aggregation Enhancement Force Induces ␤-Aggregationmentioning

confidence: 81%

Amyloid-Like β-Aggregates as Force-Sensitive Switches in Fungal Biofilms and Infections

Lipke

Klotz

Dufrêne

et al. 2018

Microbiol Mol Biol Rev

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118

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Cellular aggregation is an essential step in the formation of biofilms, which promote fungal survival and persistence in hosts. In many of the known yeast cell adhesion proteins, there are amino acid sequences predicted to form amyloid-like β-aggregates. These sequences mediate amyloid formation , these sequences mediate a phase transition from a disordered state to a partially ordered state to create patches of adhesins on the cell surface. These β-aggregated protein patches are called adhesin nanodomains, and their presence greatly increases and strengthens cell-cell interactions in fungal cell aggregation. Nanodomain formation is slow (with molecular response in minutes and the consequences being evident for hours), and strong interactions lead to enhanced biofilm formation. Unique among functional amyloids, fungal adhesin β-aggregation can be triggered by the application of physical shear force, leading to cellular responses to flow-induced stress and the formation of robust biofilms that persist under flow. Bioinformatics analysis suggests that this phenomenon may be widespread. Analysis of fungal abscesses shows the presence of surface amyloids, a finding which supports the idea that phase changes to an amyloid-like state occur . The amyloid-coated fungi bind the damage-associated molecular pattern receptor serum amyloid P component, and there may be a consequential modulation of innate immune responses to the fungi. Structural data now suggest mechanisms for the force-mediated induction of the phase change. We summarize and discuss evidence that the sequences function as triggers for protein aggregation and subsequent cellular aggregation, both and .

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“…Moreover, surface expressions of Hwp1 have been studied to be more important in mucosal candidiasis (Kartasova et al 1988 ; Balish et al 2001 ). It is well known that C. albicans binds to different extracellular membrane proteins namely fibronectin, entactin, laminin and collagen, and are considered as potential target molecules when Candida spreads in the host body (Calderone 1993 ; Pendrak and Klotz 1995 ; Chaffin et al 1998 ). C. albicans has many fibronectin receptors on its surface, including 105-kDa and 60kDa glycoproteins and mammalian homologs of integrins (Sa et al 1994 ).…”

Section: Characterised Adhesins Of Common Opportunistic Human Fungal Pathogensmentioning

confidence: 99%

Adhesins in the virulence of opportunistic fungal pathogens of human

et al. 2021

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Aspergillosis, candidiasis, and cryptococcosis are the most common cause of mycoses-related disease and death among immune-compromised patients. Adhesins are cell-surface exposed proteins or glycoproteins of pathogens that bind to the extracellular matrix (ECM) constituents or mucosal epithelial surfaces of the host cells. The forces of interaction between fungal adhesins and host tissues are accompanied by ligand binding, hydrophobic interactions and protein-protein aggregation. Adherence is the primary and critical step involved in the pathogenesis; however, there is limited information on fungal adhesins compared to that on the bacterial adhesins. Except a few studies based on screening of proteome for adhesin identification, majority are based on characterization of individual adhesins. Recently, based on their characteristic signatures, many putative novel fungal adhesins have been predicted using bioinformatics algorithms. Some of these novel adhesin candidates have been validated by in-vitro studies; though, most of them are yet to be characterised experimentally. Morphotype specific adhesin expression as well as tissue tropism are the crucial determinants for a successful adhesion process. This review presents a comprehensive overview of various studies on fungal adhesins and discusses the targetability of the adhesins and adherence phenomenon, for combating the fungal infection in a preventive or therapeutic mode.

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Adherence ofCandida albicansto host cells

Cited by 31 publications

References 48 publications

An in vitro study on comparative adherence of oral Candida albicans and non–albicans Candida to human buccal epithelial cells and its antifungal susceptibility pattern

An in vitro study on comparative adherence of oral Candida albicans and non–albicans Candida to human buccal epithelial cells and its antifungal susceptibility pattern

Amyloid-Like β-Aggregates as Force-Sensitive Switches in Fungal Biofilms and Infections

Adhesins in the virulence of opportunistic fungal pathogens of human

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