Adherence of cells to myelin basic protein I. Adherence of red and white blood cells from patients with multiple sclerosis to myelin basic protein

Najbauer, Joseph; Kosaras, Béla; Tigyi, Gábor

doi:10.1111/j.1600-0404.1987.tb03563.x

Cited by 2 publications

(1 citation statement)

References 12 publications

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“…Immunolabeling of myelin basic protein (MBP) consisted of incubation of slides in a blocking agent (5% normal donkey serum + 0.5% triton X‐100 in 0.1 M phosphate buffer (PB; CSHP pdb.rec12291 ) followed by an overnight incubation in anti‐MBP primary antibody (Millipore Cat# MAB386 RRID: AB_94975, 1:2000). Anti‐MBP is also known to cross‐react with red blood cells within brain vasculature (Eylar, ; Najbauer, Kosaras, & Tigyi, ). The next day, the slides were washed in 0.1 M PB, followed by incubation in the appropriate fluorescently conjugated donkey anti rat secondary antibody (Thermo Fisher Scientific Cat# A‐21208, RRID:AB_2535794, lot #1789917 or Thermo Fisher Scientific Cat# A‐21209, RRID:AB_2535795, lot #1807726, 1:200) for 3 hr.…”

Section: Methodsmentioning

confidence: 99%

A novel cerebellar commissure and other myelinated axons in the Purkinje cell layer of a pond turtle (Trachemys scripta elegans)

Daly

Ariel

2018

J of Comparative Neurology

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Parallel fibers in the molecular layer of the vertebrate cerebellum mediate slow spike conduction in the transverse plane. In contrast, electrophysiological recordings have indicated that rapid spike conduction exists between the lateral regions of the cerebellar cortex of the red-ear pond turtle (Trachemys scripta). The anatomical basis for this commissure is now examined in that species using neuronal tracing techniques. Fluorescently tagged dextrans and lipophilic carbocyanine dyes placed in one lateral edge of this nonfoliated cortex are transported across the midline of living brains in vitro and along the axonal membranes of fixed tissues, respectively. Surprisingly, the labeled commissural axons traversed the cortex within the Purkinje cell layer, and not in the white matter of the molecular layer or the white matter below the granule cell layer. Unlike thin parallel fibers that exhibit characteristic varicosities, this commissure is composed of smooth axons of large diameter that also extend beyond the cerebellar cortex via the cerebellar peduncles. Double labeling with myelin basic protein antibody demonstrated that these commissural axons are ensheathed with myelin. In contrast to this transverse pathway, an orthogonal myelinated tract was observed along the cerebellar midline. The connections of this transverse commissure with the lateral cerebellum, the vestibular nuclear complex, and the cochlear vestibular ganglia indicate that this commissure plays a role in bilateral vestibular connectivity.

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Section: Methodsmentioning

confidence: 99%

A novel cerebellar commissure and other myelinated axons in the Purkinje cell layer of a pond turtle (Trachemys scripta elegans)

Daly

Ariel

2018

J of Comparative Neurology

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Adherence of cells to myelin basic protein II. Adherence of red blood cells of SJL mice with chronic relapsing EAE

Najbauer

Bozóky

Tigyi

1987

Acta Neurologica Scandinavica

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Adherence of red blood cells from SJL mice suffering of chronic relapsing experimental allergic encephalomyelitis was studied to myelin basic protein coated microtiter plates. Control animals received either bovine serum albumine or "protein-antigen free" adjuvant using the same immunization protocol. Characteristic changes in adherence were found in bovine or human myelin basic protein injected animals compared to the bovine serum albumine immunized group. After a nonspecific increase in adherence between Days 2 to 6 observed in all 3 groups, in the encephalitogen challenged animals on Days 13-14 a marked decrease in red blood cell adherence was detected which maintained at this decreased level during the clinically active stage of the disease and reappeared with the relapse of EAE. No such decreased adherence of red blood cells was observed in BSA immunized animals or in adherence of cells from myelin basic protein injected animals to other basic type protein such as histone. Thus, decreased adherence of red blood cells in animals with EAE appears to be an interestingly unique measure of the disease activity.

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Adherence of cells to myelin basic protein I. Adherence of red and white blood cells from patients with multiple sclerosis to myelin basic protein

Cited by 2 publications

References 12 publications

A novel cerebellar commissure and other myelinated axons in the Purkinje cell layer of a pond turtle (Trachemys scripta elegans)

A novel cerebellar commissure and other myelinated axons in the Purkinje cell layer of a pond turtle (Trachemys scripta elegans)

Adherence of cells to myelin basic protein II. Adherence of red blood cells of SJL mice with chronic relapsing EAE

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