Adherence and Pathogenesis of <i>Salmonella enteritidis</i> in Mice

Aslanzadeh, Jaber; Paulissen, Leo J.

doi:10.1111/j.1348-0421.1990.tb01067.x

Cited by 18 publications

(9 citation statements)

References 23 publications

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“…These include some of the more virulent and troublesome microorganisms that afflict the human host, such as Vibrio cholerae (4), Salmonella spp. (3,9), Bordetella pertussis (6), and Escherichia coli (10). It is thus reasonable to expect that the hemagglutinins of P. gingivalis are also involved in virulence.…”

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confidence: 99%

The hemagglutinin gene A (hagA) of Porphyromonas gingivalis 381 contains four large, contiguous, direct repeats

1996

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Porphyromonas gingivalis is a gram-negative anaerobic bacterial species strongly associated with adult periodontitis. One of its distinguishing characteristics and putative virulence properties is the ability to agglutinate erythrocytes. We have previously reported the cloning of multiple hemagglutinin genes from P. gingivalis 381. Subsequent sequencing of clone ST 2 revealed that the cloned fragment contained only an internal portion of the gene which lacked both start and stop codons. We here report the cloning and sequencing of the entire gene, designated hagA, as well as its relationship to other genes of this species. By use of inverse PCR technology and the construction of several additional genomic libraries, the complete open reading frame of hagA was found to be 7,887 bp in length, encoding a protein of 2,628 amino acids with a molecular mass of 283.3 kDa, which is among the largest genes ever cloned from a prokaryote to date. Within its open reading frame, four large, contiguous, direct repeats (varying from 1,318 to 1,368 bp) were identified. The repeat unit (HArep), which is assumed to contain the hemagglutinin domain, is also present in other recently reported protease and hemagglutinin genes in P. gingivalis. Thus, we propose that hagA and the other genes which share the HArep sequence form a multigene family with hagA as a central member.

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confidence: 99%

The hemagglutinin gene A (hagA) of Porphyromonas gingivalis 381 contains four large, contiguous, direct repeats

1996

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“…However, the bacterial adherence and subsequent invasion are believed to be important steps in the process of Salmonella infection (9,18). Although the type 1 pili of some of the Salmonella serovars are known to be highly adhesive to HeLa and HEp-2 cells (1,5,19), there are only a few reports on the role of pili in invasion of S. typhimurium to cultured cells (9). Ernst et al (6) reported that type 1 pili of S. typhimurium contribute to the bacterial invasion to HEp-2 cells.…”

Section: Introductionmentioning

confidence: 99%

Type 1 Pili Enhance the Invasion of Salmonella braenderup and Salmonella typhimurium to HeLa Cells

Horiuchi

Inagaki

Okamura

et al. 1992

Microbiology and Immunology

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The relationship between type 1 pili-associated adhesion and invasion to HeLa cells by Salmonella braenderup and S. typhimurium was studied.When the clinical isolates of these strains were grown in L-broth, they showed both type 1 pili formation and mannose-sensitive adhesion to HeLa cells. On the other hand, the type 1 pili-defective mutants, which were obtained either by repeated subcultures on L-agar plates or by the transposon Tn1-insertion mutagenesis of the S. braenderup and S. typhimurium strains, concomitantly lost mannose-sensitive adhesion to HeLa cells. When the HeLa cells were incubated with Salmonella, the type 1 piliated strains invaded the HeLa cells with much higher infection rate than did the type 1 pilidefective strains.The invasion of type 1 piliated strains to HeLa cells was markedly inhibited in the presence of D-mannose.The infectivity of the strain, which lost type 1 pili but still had mannose-resistant adhesion, was slightly higher than that of the strains defective in both mannose-sensitive and mannose-resistant adhesion. These results suggested that type 1 pili have a role in enhancing the invasion of S. braenderup and S. typhimurium to HeLa cells.

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“…Hemagglutinins specific for human erythrocytes have also been described previously in Escherichia coli (32,34). In addition, Salmonella strains able to agglutinate erythrocytes from a range of animals, or which show only weak hemagglutination of human erythrocytes have been reported (2,3).…”

Section: Resultsmentioning

confidence: 99%

Hemagglutinating properties of Salmonella enterica serovar Enteritidis isolated from different sources

Mikcha

Ferreira

et al. 2004

Braz. J. Microbiol.

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Twenty-five strains of Salmonella enterica serovar Enteritidis isolated from different sources were examined for hemagglutinating activity. Bacteria cultured in different media induced hemagglutination of human erythrocytes, but no reaction was observed with erythrocytes from other animal species. The hemagglutinating expression activity was better for cultures on CFA agar at 37ºC than other conditions examined. The hemagglutination was inhibited by D-mannose, D-mannitol, melibiose, D-raffinose, L-rhamnose and sucrose. The absence of cell-surface appendages in electron microscope examinations suggested a nonfimbrial hemagglutinin. The data suggest that Salmonella Enteritidis produces nonfimbrial mannose-sensitive hemagglutinin, specific for human erythrocytes, which could be extracted in soluble form.

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Adherence and Pathogenesis of Salmonella enteritidis in Mice

Abstract: Adherence of many pathogenic organisms to the host cells has been associated with the presence of fimbriae.

Cited by 18 publications

References 23 publications

The hemagglutinin gene A (hagA) of Porphyromonas gingivalis 381 contains four large, contiguous, direct repeats

The hemagglutinin gene A (hagA) of Porphyromonas gingivalis 381 contains four large, contiguous, direct repeats

Type 1 Pili Enhance the Invasion of Salmonella braenderup and Salmonella typhimurium to HeLa Cells

Hemagglutinating properties of Salmonella enterica serovar Enteritidis isolated from different sources

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