Adherence and invasion of Bacteroidales isolated from the human intestinal tract

Nakano, Viviane; Piazza, Roxane M. F.; Cianciarullo, Aurora Marques; Bueris, Vanessa; Santos, M. F.; Menezes, M.A.; Mendes-Ledesma, Meire R. B.; Szulczewski, V; Elias, Waldir P.; Pumbwe, Lilian; Wexler, Hannah M.; Avila-Campos, Mario Júlio

doi:10.1111/j.1469-0691.2008.02069.x

Cited by 17 publications

(14 citation statements)

References 32 publications

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“…This important finding has been achieved both in vitro on EPCs by confocal microscopy (Figure 1) and in vivo on infected mice tissues analyzed by ultrastructural analysis (Figure 6 and S6). To our knowledge, TEM examination has been used only in a few in vitro studies to explore cell internalization of B. fragilis but never for B. fragilis ΔPSA [32], [33]. A previous study showing the ability of B. fragilis PSA to stimulate an anti-inflammatory background in the intestine suggested that PSA may be critical for the establishment of a commensal intestinal flora [34].…”

Section: Discussionmentioning

confidence: 99%

Evidence of Bacteroides fragilis Protection from Bartonella henselae-Induced Damage

et al. 2012

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Bartonella henselae is able to internalize endothelial progenitor cells (EPCs), which are resistant to the infection of other common pathogens. Bacteroides fragilis is a gram-negative anaerobe belonging to the gut microflora. It protects from experimental colitis induced by Helicobacter hepaticus through the polysaccharide A (PSA). The aim of our study was to establish: 1) whether B. fragilis colonization could protect from B. henselae infection; if this event may have beneficial effects on EPCs, vascular system and tissues. Our in vitro results establish for the first time that B. fragilis can internalize EPCs and competes with B. henselae during coinfection. We observed a marked activation of the inflammatory response by Real-time PCR and ELISA in coinfected cells compared to B. henselae-infected cells (63 vs 23 up-regulated genes), and after EPCs infection with mutant B. fragilis ΔPSA (≅90% up-regulated genes) compared to B. fragilis. Interestingly, in a mouse model of coinfection, morphological and ultrastructural analyses by hematoxylin-eosin staining and electron microscopy on murine tissues revealed that damages induced by B. henselae can be prevented in the coinfection with B. fragilis but not with its mutant B. fragilis ΔPSA. Moreover, immunohistochemistry analysis with anti-Bartonella showed that the number of positive cells per field decreased of at least 50% in the liver (20±4 vs 50±8), aorta (5±1 vs 10±2) and spleen (25±3 vs 40±6) sections of mice coinfected compared to mice infected only with B. henselae. This decrease was less evident in the coinfection with ΔPSA strain (35±6 in the liver, 5±1 in the aorta and 30±5 in the spleen). Finally, B. fragilis colonization was also able to restore the EPC decrease observed in mice infected with B. henselae (0.65 vs 0.06 media). Thus, our data establish that B. fragilis colonization is able to prevent B. henselae damages through PSA.

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Section: Discussionmentioning

confidence: 99%

Evidence of Bacteroides fragilis Protection from Bartonella henselae-Induced Damage

et al. 2012

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“…Although adhesion is essential for maintaining members of the normal microflora in their host, it is also the crucial first stage in any infectious disease (Wilson, 2002). The capsular polymer can first protect B. fragilis cells from phagocytosis and then, probably when produced in larger amounts, stabilize adhesion of cells to epithelia (Nakano et al , 2008). In this study, all the examined B. fragilis strains were encapsulated, and these results are according to other studies (Domingues et al , 1995; Nakano and Avila-Campos, 2004).…”

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confidence: 99%

Virulence factors and biofilm production by isolates of Bacteroides fragilis recovered from dog intestinal tracts

Reis¹,

Silva²,

Laranjeira³

et al. 2014

Braz. J. Microbiol.

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Bacteroides fragilis colonizes dog guts both as a commensal and as an opportunistic pathogen. This study aims to evaluate virulence factors of 13 B. fragilis strains isolated from dog intestinal tracts and their ability for biofilm formation. Capsules were detected in all the evaluated strains. A total of 61.5% of all strains were biofilm producers. These attributes most likely play an important role in B. fragilis persistent colonization in the gut.

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“…Moreover, it has been shown that the polysaccharide capsule is considered the major determinants in pathogen virulence [13]. It is probable that the capsular polysaccharide, when produced in high amounts, alleviates cells' adhesion to epithelia [14]. Other authors reported capsule production allows the bacterium to develop at the infection site [15] and, through its biopolymer components, contributes to the formation of biofilm [16].…”

Section: Introductionmentioning

confidence: 99%

Interaction of Vibrio to Biotic and Abiotic Surfaces: Relationship between Hydrophobicity, Cell Adherence, Biofilm Production, and Cytotoxic Activity

2018

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Vibrio parahaemolyticus and Vibrio alginolyticus are important pathogenic agents for both humans and aquatic animals. Twenty-five bacterial strains were isolated from infected sea bass (Dicentrarchus labrax) on thiosulfate citrate bile salts sucrose (TCBS) agar plates. For the species-specific detection of V. alginolyticus and V. parahaemolyticus, a multiplex PCR assay using two collagenase-targeted primer pairs allows the detection of four strains of V. parahaemolyticus and three strains of V. alginolyticus. The seven identified isolates were partitioned for capsule production, hydrophobicity, adherence, biofilm formation, invasion, and cytotoxicity against Hep-2 cells. Two V. parahaemolyticus (Spa2 and Spa3) and one V. alginolyticus (Va01) were capsule producers developing almost black colonies on CRA, they showed a strong hydrophobicity using bacterial adhesion to hydrocarbons test (BATH), and were able to produce high biofilm. Isolates were able to adhere and invade Hep-2 cells and exhibited dissimilar levels of cytotoxicity in epithelial cells. This study shows the strong relationship between adhesion, biofilm formation, invasion and the cytotoxicity of Vibrio strains. Thus, we found a strong and significant positive correlation between different virulence properties of these isolates. The present study shows that bacterial contact with the cells as well as adhesion and invasion are essential steps to induce cytotoxicity. However, the invasion is seen to be a post adherence event.

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Adherence and invasion of Bacteroidales isolated from the human intestinal tract

Cited by 17 publications

References 32 publications

Evidence of Bacteroides fragilis Protection from Bartonella henselae-Induced Damage

Evidence of Bacteroides fragilis Protection from Bartonella henselae-Induced Damage

Virulence factors and biofilm production by isolates of Bacteroides fragilis recovered from dog intestinal tracts

Interaction of Vibrio to Biotic and Abiotic Surfaces: Relationship between Hydrophobicity, Cell Adherence, Biofilm Production, and Cytotoxic Activity

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