Adherence and Growth of Coagulase-Negative Staphylococci on Surfaces of Intravenous Catheters

Peters, Georg; Locci, R.; Pulverer, G.

doi:10.1093/infdis/146.4.479

Cited by 549 publications

(252 citation statements)

References 1 publication

Supporting

Mentioning

228

Contrasting

Unclassified

Order By: Relevance

“…In relation to the distribution of slimeproducing species of clinical source, the results are similar to those reported by other authors (8,27), who found that S. epidermidis frequently infects biomedical implants like intravascular catheters and prosthetic valves. It has also been reported (4,24) that most of the isolates of this species from such devices produce a great quantity of extracellular mucoid substance or slime, which was also detected in this study.…”

Section: Discussionsupporting

confidence: 87%

Species identification, slime production and oxacillin susceptibility in coagulase-negative staphylococci isolated from nosocomial specimens

Alcaráz

Satorres

Lucero

et al. 2003

Braz. J. Microbiol.

View full text Add to dashboard Cite

Ninety-two coagulase negative staphylococci (CNS) (forty-five of clinical origin and forty-seven of environmental origin), collected in a hospital in San Luis, Argentina, from March to June, 1999, were identified to species level by the ID 32 Staph and API Staph System (bioMérieux). Slime production was investigated by the quantitative and qualitative methods. Oxacillin susceptibility was determined by the disk diffusion test (1 µg), the agar dilution method (0.125 to 4 mg/ml) and agar screen (6 µg/ml). The presence of mecA gene was investigated by PCR. The clinical CNS species most commonly isolated were S. epidermidis, S. haemolyticus, S. hominis and S. saprophyticus. The frequency of slime production by clinical and environmental isolates was similar (25/45 and 27/47, respectively) and the results obtained by the quantitative and the qualitative methods correlated well. The mecA gene was detected in all S. epidermidis, S. haemolyticus and S. hominis isolates, which were resistant to oxacillin by the phenotypic methods. However, this gene was not present in S. klossii, S. equorum, S. xylosus and S. capitis strains. The gene was neither found in two out of the six S. saprophyticus isolates, in two out of three S. cohnii subsp. urealyticum isolates and in two out of five S. cohnii subsp. cohnii isolates, all of which resulted oxacillin resistant according to MIC. The gene was not found in oxacillin-susceptible strains either. Most of the CNS isolates (enviromental and clinical) that were slime producers were found to be oxacillin resistant, which makes the early detection of these microorganisms necessary to prevent their dissemination in hospitals, particularly among immunocompromised patients.

show abstract

Section: Discussionsupporting

confidence: 87%

Species identification, slime production and oxacillin susceptibility in coagulase-negative staphylococci isolated from nosocomial specimens

Alcaráz

Satorres

Lucero

et al. 2003

Braz. J. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Although rapid detection of S. aureus in clinical specimens is essential for appropriate patient care, the recovery and identification of other staphylococci is also important, especially from catheters and other foreign-body samples and blood cultures (16)(17)(18)(19)(20)(21). Culture-based detection methods are cost-effective and useful, especially when various microbe species are examined.…”

Section: Discussionmentioning

confidence: 99%

Performance of Sa Select , a Chromogenic Medium for Detection of Staphylococci in Clinical Specimens

Hirvonen¹,

Kerttula²,

Kaukoranta³

2014

J Clin Microbiol

View full text Add to dashboard Cite

bIn a preliminary study, known staphylococcus (n ‫؍‬ 86) and other microbial (n ‫؍‬ 12) isolates were plated on three chromogenic media, SaSelect (Bio-Rad, Hercules, CA, USA), CHROMagar Staph. aureus (CHROMagar Microbiology, Paris, France), and S. aureus ID (bioMérieux, Marcy l'Etoile, France). The sensitivities of all the media to detect Staphylococcus aureus after 24 h of incubation were high (100.0%). However, their specificities varied at 93.3% (95% confidence interval [CI], 86.0% to 100.0%) (CHROMagar Staph. aureus), 97.8% (95% CI, 93.5% to 100.0%) (S. aureus ID), and 100.0% (SaSelect). SaSelect also showed the highest sensitivity for recovery and differentiation of other staphylococci. As the best performing chromogenic medium, SaSelect was then prospectively compared to conventional culture and identification tests for the detection of staphylococci from 2,780 clinical specimens. A total of 1,589 staphylococcal isolates were recovered. Of these, 912 were S. aureus and 677 were other staphylococci. The sensitivity and specificity of SaSelect to detect S. aureus in clinical specimens after 24 h of incubation were 99.6% and 99.9% (95% CI, 99.2% to 100.0% and 99.8% to 100.0%), respectively, whereas the sensitivity and specificity using conventional plates combined with laboratory identification methods were 96.8% and 99.5% (95% CI, 95.7 to 97.9% and 99.2% to 99.8%). For the recovery and preliminary identification of other staphylococci, the sensitivity and specificity of SaSelect were 94.4% and 99.9%. SaSelect is a well-performing chromogenic medium that significantly improved the detection of staphylococci, especially S. aureus, compared to conventional culture (P < 0.0001).

show abstract

“…On the other hand, surface defects may be secondary to microbial colonisation. This theory is supported by reports that some strains of S. epidermidis may cause erosion of the surface of a variety of cannula types, including those made of Teflon (Peters et al, 1982).…”

Section: Discussionmentioning

confidence: 71%

“…Recent in-vitro studies have suggested that surface defects in the cannulae favour bacterial attachment and subsequent colonisation (Locci,Peters and Pulverer,198 lb). The production of slime and the breakdown of catheter components by bacteria may promote such colonisation (Peters, Locci and Pulverer, 1982). Moreover, it has been shown that intravenous cannulae provoke a tissue response that results in the formation of a fibrin sleeve around the cannula (Hoshal, Ause and Hoskins, 1971).…”

Section: Introductionmentioning

confidence: 99%

A Morphological Study of Bacterial Colonisation of Intravenous Cannulae

Cheesbrough¹,

Elliott²,

Finch³

1985

Journal of Medical Microbiology

View full text Add to dashboard Cite

SUMMARY. In an investigation of bacterial colonisation of intravascular cannulae, 16 peripheral venous cannulae of the Venflon variety and 24 chronic haemodialysis cannulae were studied after removal from patients. Studies of colonisation included semi-quantitative microbiological culture and scanning electronmicrographic (SEM) observations. The microbiological findings indicated colonisation of the intravascular portion of the catheter in 4 of 16 Venflon cannulae and 18 of 26 haemodialysis cannulae, largely with skin commensal organisms. The results of the SEM studies were in broad agreement with the microbiological observations. Surface defects on the cannulae were shown to be associated with microbial colonisation which occurred either as isolated colonies or in association with a cellular fibrinous matrix. These observations are illustrated and discussed.

show abstract

Adherence and Growth of Coagulase-Negative Staphylococci on Surfaces of Intravenous Catheters

Cited by 549 publications

References 1 publication

Species identification, slime production and oxacillin susceptibility in coagulase-negative staphylococci isolated from nosocomial specimens

Species identification, slime production and oxacillin susceptibility in coagulase-negative staphylococci isolated from nosocomial specimens

Performance of Sa Select , a Chromogenic Medium for Detection of Staphylococci in Clinical Specimens

A Morphological Study of Bacterial Colonisation of Intravenous Cannulae

Contact Info

Product

Resources

About