Adherence and colonization properties of Lactobacillus rhamnosus TB1, a broiler chicken isolate

Abstract

T . B O U Z A I N E , R . D . D A U P H I N , P H . T H O N A R T , M . C . U R D A C I A N D M . H A M D I . 2005.Aims: Selected lactic acid bacteria (LAB) isolated from intestinal tract of chicken have been studied in order to investigate their ability to adhere in vitro to Basement Membrane Matrigel (BMM). A selected strain showing a good adherence in BMM test was used for in vivo colonization assays. Methods and Results: In vitro assessment of adhesion of broiler chicken isolates was performed using BMM assay. Among LAB strains tested, Lactobacillus rhamnosus TB1 showed a good adherence that was comparable to the one of an Escherichia coli EPEC strain used as positive control. For in vivo colonization assays this strain was fluorescently stained with the carboxyfluorescein diacetate succinimidyl ester (cFDA-SE) thus allowing its detection in different layers of intestinal tract after inoculation in broiler chicken. Further, stained L. rhamnosus were found with a highest value in rectum, jejunum and ileum both 3 and 24 h after administration. Conclusions: BMM assay is a quick method to test in vitro adhesion properties of bacterial strains and cFDA-SE-stained bacteria may be considered as an alternative method to test in vivo adhesion and colonization properties. Significance and Impact of the Study: Lactobacillus rhamnosus TB1 was therefore showed to be able to adhere strongly in vitro to BMM and in vivo to intestinal epithelial cells of chicken and may be considered as a potential probiotic for chicken.

The effects of Blood Rumen Content Mixture (BRCM) meal supplemented with yeast on the performance and gut microbial populations of broiler chickens

“…A further report by Bouzaineet al (2005) reveals that at 2 to 4 days post hatch, Streptococci and Enterobacteria colonize the small intestine and caecum. The isolation of Bacillus and Streptococcus and species of bacteria from the ileum and caecum of broiler chicken fed BRCM meal in this study corroborates the earlier reports (Cummings andMacfarlane, 1997 andBouzaine, et al, 2005).…”

“…For example, Mead (1989) reported that the major species present in the small intestines of young chicks was lactobacillus with bifidiobacteria populations becoming more dominant in the caeca at older age. Bouzaine et al (2005) also reported that after the first week, lactobacillus predominates in the small intestine. A further report by Bouzaineet al (2005) reveals that at 2 to 4 days post hatch, Streptococci and Enterobacteria colonize the small intestine and caecum.…”

Lactobacillus Strain Ecology and Persistence within Broiler Chickens Fed Different Diets: Identification of Persistent Strains

“…While several articles have described Lactobacillus strains which are able to survive and persist within the GIT of poultry (8,16,31,42,46), the persistence ranged from 1 day through 4 weeks after cessation of administration. Ehrmann et al (16) described the persistence of their two selected strains within ducks for up to 28 days using only a single dose.…”

In vitro characterization of aggregation and adhesion properties of viable and heat-killed forms of two probiotic Lactobacillus strains and interaction with foodborne zoonotic bacteria, especially Campylobacter jejuni

“…Several probiotic bacteria including bifidobacteria and lactobacilli adhere to mucosal tissue in a strain-specific manner (O'Hara & Shanahan, 2007). Selected probiotic strains should be able to compete with pathogens for the same receptors and to occupy their potential binding sites in the gut, thus representing a potential therapeutic strategy (Bouzaine et al, 2005;O'Hara & Shanahan, 2007). This enhances the intestinal persistence of the probiotic bacteria, and limits pathogen access to the epithelium (O'Hara & Shanahan, 2007).…”