Adhere today, here tomorrow: oral bacterial adherence

Kolenbrander, Paul E.; London, Jack

doi:10.1128/jb.175.11.3247-3252.1993

Cited by 614 publications

(543 citation statements)

References 46 publications

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“…P. intermedia and Prevotella nigrescens are frequently isolated from the periodontal lesions of patients with chronic periodontitis (8,9,10), aggressive periodontitis (11,12), puberty-associated gingivitis (13,14), and acute necrotizing ulcerative gingivitis (15). Recently, our research group reported that the average number of periodontopathic bacterial species, including P. intermedia and P. nigrescens, was significantly higher in plaque samples 5 harboring F. nucleatum and/or F. periodontium than in those without in Japanese children and mothers (16). Previous studies showed that P. intermedia and P. nigrescens possessed specific adherence activity (17,18), and that they were late colonizers (6).…”

Section: Introductionmentioning

confidence: 99%

“…Fusobacterium species play a central role in biofilm formation, mediating coaggregation between early and late colonizers (5,6,7). Fusobacterium species including F. nucleatum, are detected in high frequency in lesions of gingivitis and chronic periodontitis (8,9).…”

Section: Introductionmentioning

confidence: 99%

“…In dental plaque biofilm formation, early colonizers, including streptococci, adhere to pellicles, while late colonizers, including periodontopathic bacteria such as Porphyromonas gingivalis and Prevotella intermedia, adhere to antecedent microorganisms on the tooth surface (5). Fusobacterium species play a central role in biofilm formation, mediating coaggregation between early and late colonizers (5,6,7).…”

Section: Introductionmentioning

confidence: 99%

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Synergy in biofilm formation between Fusobacterium nucleatum and Prevotella species

et al. 2012

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Japan Running title: F. nucleatum-Prevotella species CoaggregationThe aim of this study was to characterize the role of coaggregation between Fusobacterium nucleatum and Prevotella species in biofilm formation. Materials and Methods:ATCC and clinically isolated strains of F. nucleatum, P.intermedia and P. nigrescens were subjected. Coaggregation between these species was determined by visual assay. Biofilm formation was assessed by crystal violet staining. Effect of co-culture of F. nucleatum with Prevotella species was evaluated. Enhancement of biofilm formation via soluble factor was also examined by addition of culture supernatant and a two-compartment separated co-culture system. Production of autoinducer-2 (AI-2) by the tested organisms was evaluated using Vibrio harveyi BB170.Results: Cells of all F. nucleatum strains coaggregated with P. intermedia or P.nigrescens. Addition of EDTA or L-lysine inhibited coaggregation, the other sugars and amino acids tested did not. Coaggregation disappeared after heating of P. intermedia and P. nigrescens cells, but not after heating of F. nucleatum cells. Proteinase K treatment of P. nigrescens cells affected coaggregation with F. nucleatum, but no significant effect was observed with the treatment of cells of P. intermedia or F. nucleatum. The quantities of biofilm formation by strains of F. nucleatum, P. intermedia and P. nigrescens varied.Co-culture of F. nucleatum ATCC 25586 with P. intermedia or P. nigrescens strains increased biofilm formation than by the single culture (p<0.05).Production levels of AI-2 in strains of F. nucleatum, P. intermedia and P.3 nigrescens varied. Addition of culture supernatant of P. intermedia or P.nigrescens did not enhance biofilm formation by F. nucleatum, and no biofilm formation enhancement by F. nucleatum was observed by the two-compartment co-culture system. Conclusion:These findings indicate that physical contact by coaggregation of F.nucleatum strains with P. intermedia or P. nigrescens is the key factor in the formation of biofilm by these strains, rather than the AI-2 signaling molecule. 4 IntroductionDental plaque, a multispecies biofilm, is organized on the tooth surfaces and periodontal tissues of the human oral cavity (1). More than 500 taxa of bacteria have been identified in the human oral cavity (2), and an increase in specific anaerobic, Gram-negative bacteria in the biofilm is involved in the progression of periodontal disease (3). Bacterial gene expression in the biofilm is regulated by quorum sensing according to increase in cell density (1). After incorporation into the biofilm, microorganisms become resistant not only to host defense mechanisms, but also antimicrobial agents. Moreover, this change has been suggested to be involved in the persistence of infection by such microorganisms in the subgingival crevice (4).In dental plaque biofilm formation, early colonizers, including streptococci, Coaggregation assayVisual assay of coaggregation of F. nucleatum with P. intermedia or P. leaving a water-clear supernatant...

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Synergy in biofilm formation between Fusobacterium nucleatum and Prevotella species

et al. 2012

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“…2014)tooth, gingival crevice and dorsum linguae, etc.) [13], and sterilization of the all oral bacteria is very hard. Hence, we believe that the decrease in inactivation to 80 % is adequate results for the oral biocidal activity.…”

Section: Resultsmentioning

confidence: 99%

Micro-Biocidal Activity of Yeast Cells by Needle Plasma Irradiation at Atmospheric Pressure

Kurumi

Takahashi²,

Taima³

et al. 2014

Proceedings of the 12th Asia Pacific Physics Conference (APPC12)

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In this study, we report on the biocidal activity technique by needle helium plasma irradiation at atmospheric pressure using borosilicate capillary nozzle to apply for the oral surgery. The diameter of needle plasma was less than 50 m, and temperature of plasma irradiated area was less than body temperature. Needle plasma showed emission due to OH and O radical. Raman spectra and methylene blue stain showed yeast cells were inactivated by needle plasma irradiation.

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“…The significance of F. nucleatum in the development of the periodontal disease, as well as infections in other organs, has generated interest due to its pathogenic potential, its frequency in periodontal lesions, its synergism in mixed infections and its ability to form aggregates with other suspect pathogens in the periodontal disease 7,12 .…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity of oral Fusobacterium nucleatum isolated from patients with different clinical conditions

Ávila-Campos

Rivera

Nakano

2006

Rev. Inst. Med. trop. S. Paulo

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SUMMARYThe genetic diversity of 23 oral Fusobacterium nucleatum isolated from 15 periodontal patients, eight from seven healthy subjects, nine from nine AIDS patients and two from two Cebus apella monkeys were analyzed. EcoRI restricted the bacterial DNA and 28 ribotypes grouped from A to J groups were obtained. Isolates formed 24 ribotypes which were contained into A, B, C, D, E and F groups, and three reference strains and two clinical isolates of A. actinomycetemcomitans, and E. coli CDC formed four different ribotypes into the G, H, I and J groups. Moreover, from nine F. nucleatum from AIDS patients, six were ribotyped as group C and three as group D. By using ribotyping we distinguished F. nucleatum recovered from different sources. It is possible that isolates from AIDS patients may contain some phenotypic or genotypic factor did not observed in this study.

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Adhere today, here tomorrow: oral bacterial adherence

Cited by 614 publications

References 46 publications

Synergy in biofilm formation between Fusobacterium nucleatum and Prevotella species

Synergy in biofilm formation between Fusobacterium nucleatum and Prevotella species

Micro-Biocidal Activity of Yeast Cells by Needle Plasma Irradiation at Atmospheric Pressure

Genetic diversity of oral Fusobacterium nucleatum isolated from patients with different clinical conditions

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