Adenylyl cyclase isoforms in rat testis and spermatozoa from the cauda epididymidis

Wade, M. A.; Roman, Shaun D.; Jones, R. C.; Aitken, R. John

doi:10.1007/s00441-003-0801-6

Cited by 19 publications

(9 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Round and elongating spermatids possess mRNA for the olfactory ADCY3 and for the G aolf homologue of G s [36][37][38]. Western blot analysis and immunocytochemistry indicated that post-meiotic germ cells also may produce the ADCY3 protein [49,50]. However, a role for ADCY3 in mature sperm remains controversial.…”

Section: No Involvement Of Transmembrane Adenylyl Cyclasementioning

confidence: 94%

Signaling Pathways for Modulation of Mouse Sperm Motility by Adenosine and Catecholamine Agonists1

Schuh¹,

Carlson²,

McKnight³

et al. 2006

Biology of Reproduction

View full text Add to dashboard Cite

Capacitation of mammalian sperm, including alterations in flagellar motility, is presumably modulated by chemical signals encountered in the female reproductive tract. This work investigates signaling pathways for adenosine and catecholamine agonists that stimulate sperm kinetic activity. We show that 2-chloro-2'-deoxyadenosine and isoproterenol robustly accelerate flagellar beat frequency with EC50s near 10 and 0.05 microM, respectively. The several-fold acceleration is maximal by 60 sec. Although extracellular Ca2+ is required for agonist action on the flagellar beat, agonist treatment does not elevate sperm cytosolic [Ca2+] but does increase cAMP content. Acceleration does not require the conventional transmembrane adenylyl cyclase ADCY3, since it persists in sperm of ADCY3 knockout mice and in wild-type sperm in the presence of the inhibitors of conventional adenylyl cyclases SQ-22536, MDL-12330A, or 2', 5'-dideoxyadenosine. In contrast, the acceleration by these agents is absent in sperm that lack the predominant atypical adenylyl cyclase, SACY. Responses to these agonists are also absent in sperm from mice lacking the sperm-specific Calpha2 catalytic subunit of protein kinase A (PRKACA). Agonist responses also are strongly suppressed in wild-type sperm by the protein kinase inhibitor H-89. These results show that adenosine and catecholamine analogs activate sperm motility by mechanisms that require extracellular Ca2+, the atypical sperm adenylyl cyclase, cAMP, and protein kinase A.

show abstract

Section: No Involvement Of Transmembrane Adenylyl Cyclasementioning

confidence: 94%

Signaling Pathways for Modulation of Mouse Sperm Motility by Adenosine and Catecholamine Agonists1

Schuh¹,

Carlson²,

McKnight³

et al. 2006

Biology of Reproduction

View full text Add to dashboard Cite

show abstract

“…To confirm the presence of mRNA during RT, adenyl cyclase III was selected to act as a control gene as its presence had previously been identified in rat caudal epididymal spermatozoa (Wade et al 2003). Additionally, it was probable that this particular splice variant would be present in rat MII oocytes and liver.…”

Section: Primer Sequencementioning

confidence: 99%

The effect of glycosaminoglycans on rat gametes in vitro and the associated signal pathway

Borg

Holland²

2008

Reproduction

View full text Add to dashboard Cite

The effects of adding the extracellular glycosaminoglycans (GAGs), hyaluronic acid (HA) and chondroitin sulphate (CS) to rat in vitro fertilisation (IVF) media were assessed. Metaphase II (MII) oocytes were also incubated in GAG-supplemented modified rat 1-cell embryo culture medium (mR1ECMCBSA) for 3 days. Cytoplasmic fragmentation was significantly reduced in mR1ECMCBSA with HA (39.0-48.0%) compared with the control (82.0%). In IVF experiments, neither HA (8.0-30.8%) nor CS (9.7-42.5%) improved fertilisation rates compared with controls fertilised in M16 (47.2%) or enriched Krebs-Ringer bicarbonate solution (61.5%). RT-PCR and Western blot were used to probe for CD44 mRNA and protein in Sprague-Dawley gametes and cumulus cells. CD44 was identified in cumulus cells, suggesting a role for oocyte maturation and cumulus expansion. The CD44 protein was also present on caudal epididymal spermatozoa that were highly stimulated by CS in vitro implicating a role in fertilisation for CS and CD44.

show abstract

“…Proteins that are differently phosphorylated with or without AC inhibitors may be the key to prove this model. Several studies demonstrate distinct localization of AC isoforms in mammalian [40,41,42] and sea urchin sperm [14]. Studies on immunolocalization of ACs in Ciona sperm would surely provide further evidence for the roles of ACs in the regulation of sperm motility.…”

Section: Resultsmentioning

confidence: 99%

Distinct Roles of Soluble and Transmembrane Adenylyl Cyclases in the Regulation of Flagellar Motility in Ciona Sperm

Shiba

Inaba

2014

IJMS

View full text Add to dashboard Cite

Adenylyl cyclase (AC) is a key enzyme that synthesizes cyclic AMP (cAMP) at the onset of the signaling pathway to activate sperm motility. Here, we showed that both transmembrane AC (tmAC) and soluble AC (sAC) are distinctly involved in the regulation of sperm motility in the ascidian Ciona intestinalis. A tmAC inhibitor blocked both cAMP synthesis and the activation of sperm motility induced by the egg factor sperm activating and attracting factor (SAAF), as well as those induced by theophylline, an inhibitor of phoshodiesterase. It also significantly inhibited cAMP-dependent phosphorylation of a set of proteins at motility activation. On the other hand, a sAC inhibitor does not affect on SAAF-induced transient increase of cAMP, motility activation or protein phosphorylation, but it reduced swimming velocity to half in theophylline-induced sperm. A sAC inhibitor KH-7 induced circular swimming trajectory with smaller diameter and significantly suppressed chemotaxis of sperm to SAAF. These results suggest that tmAC is involved in the basic mechanism for motility activation through cAMP-dependent protein phosphorylation, whereas sAC plays distinct roles in increase of flagellar beat frequency and in the Ca2+-dependent chemotactic movement of sperm.

show abstract

Adenylyl cyclase isoforms in rat testis and spermatozoa from the cauda epididymidis

Cited by 19 publications

References 55 publications

Signaling Pathways for Modulation of Mouse Sperm Motility by Adenosine and Catecholamine Agonists1

Signaling Pathways for Modulation of Mouse Sperm Motility by Adenosine and Catecholamine Agonists1

The effect of glycosaminoglycans on rat gametes in vitro and the associated signal pathway

Distinct Roles of Soluble and Transmembrane Adenylyl Cyclases in the Regulation of Flagellar Motility in Ciona Sperm

Contact Info

Product

Resources

About