Adenylyl Cyclase-Associated Protein 1 Is a Receptor for Human Resistin and Mediates Inflammatory Actions of Human Monocytes

Lee, Sahmin; Lee, Hyun Chae; Kwon, Yoo Wook; Lee, Sang Eun; Cho, Youngjin; Kim, Joonoh; Lee, Soobeom; Kim, Ju Young; Lee, Jaewon; Yang, Han Mo; Mook‐Jung, Inhee; Nam, Ky Youb; Chung, Junho; Lazar, Mitchell A.; Kim, Hyo Soo

doi:10.1016/j.cmet.2014.01.013

Cited by 220 publications

(237 citation statements)

References 45 publications

Supporting

Mentioning

212

Contrasting

Unclassified

Order By: Relevance

“…Previous research found no evidence for a direct interaction between resistin and TLR4 in a biochemical binding assay (22). Decorin and ROR1 were only expressed at low levels in human monocytes and neither expression level increased following resistin stimulation (22). Therefore, the inflammatory response induced by resistin was not directly related to TLR4, ROR1 or decorin.…”

Section: Discussionmentioning

confidence: 80%

“…However, none of these are considered to be the specific receptor of resistin in vivo (22). Previous research found no evidence for a direct interaction between resistin and TLR4 in a biochemical binding assay (22). Decorin and ROR1 were only expressed at low levels in human monocytes and neither expression level increased following resistin stimulation (22).…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…However, another study reported that the inflammatory response induced by resistin has no direct link with the TLR4 pathway (22). Other previous reports have stated that resistin is related to the insulin-like growth factor 1 receptor and receptor tyrosine kinase-like orphan receptor 1 (ROR1) (22)(23)(24). However, the specific receptor of resistin and its signaling pathway have not yet been identified in vivo.…”

Section: Introductionmentioning

confidence: 91%

“…A previous study reported that the resistin-induced inflammatory response may involve the Toll-like receptor 4 (TLR4) signaling pathway (21). However, another study reported that the inflammatory response induced by resistin has no direct link with the TLR4 pathway (22). Other previous reports have stated that resistin is related to the insulin-like growth factor 1 receptor and receptor tyrosine kinase-like orphan receptor 1 (ROR1) (22)(23)(24).…”

Section: Introductionmentioning

confidence: 92%

See 4 more Smart Citations

Resistin increases the expression of NOD2 in mouse monocytes

Ren

Taomei

Zuo

et al. 2017

Experimental and Therapeutic Medicine

View full text Add to dashboard Cite

Abstract. Previous studies have indicated that resistin, a type of adipokine, contributes to the development of insulin resistance and type 2 diabetes mellitus, and mediates inflammatory reactions. However, a specific receptor for resistin has not yet been identified. In this study, the relationship between resistin and nucleotide-binding oligomerization domain-like receptors, as well as resistin signal transduction, was examined through transfection, quantitative polymerase chain reaction, western blot analysis and ELISA. The mRNA expression of nucleotide-binding oligomerization domain-containing protein 2 (NOD2), a key immune receptor related to insulin resistance, was significantly increased by resistin treatment at concentrations of 100, 150 and 200 ng/ml (P<0.05, P<0.01 and P<0.01, respectively). The mRNA expression of downstream signaling molecules in the NOD2 signaling pathway, receptor-interacting serine/threonine-protein kinase 2 (RIP2; P<0.01 at 6, 12 and 24 h) and inhibitor of NF-κB kinase subunit beta (P<0.01 at 3, 6, 12 and 24 h) were significantly increased by resistin treatment compared with the control. The mRNA expression of key proinflammatory cytokines, tumor necrosis factor α, IL (interleukin)-6 and IL-1β, were also significantly increased by resistin treatment compared with the control (P<0.01). NOD2 knockdown by small interfering RNA (siRNA) significantly decreased the expression of NOD2 and RIP2 (P<0.01), and there was no significant increase in the levels of cytokines, as compared with treatment with control siRNA. These findings indicate that the inflammatory reaction induced by resistin involves the NOD2-nuclear factor (NF)-κB signaling pathway. The inhibition of NF-κB significantly decreased the secretion of key inflammatory cytokines (P<0.01), suggesting that NF-κB signaling mechanisms are essential to the resistin-induced inflammatory response.

show abstract

Section: Discussionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 92%

See 3 more Smart Citations

Resistin increases the expression of NOD2 in mouse monocytes

Ren

Taomei

Zuo

et al. 2017

Experimental and Therapeutic Medicine

View full text Add to dashboard Cite

show abstract

Resistin causes G1 arrest in colon cancer cells through upregulation of SOCS3

et al. 2017

View full text Add to dashboard Cite

Resistin, a proinflammatory cytokine, is elevated in a number of pathological disorders, including cancer. The serum resistin level in colon cancer patients is elevated and correlates with tumor grade. However, the implications of increased resistin on colon cancer cells remain unclear. In the present study, we find that resistin binds to TLR4 on colon cancer cell membrane and initiates TLR4-MyD88-dependent activation of ERK. In addition, the upregulation of SOCS3 by ERK downregulates the JAK2/TAT3 pathway and causes the arrest of cells in G1 phase. Interestingly, we observe that resistin-exposed cells survive 5-fluorouracil treatment because of a decrease in drug uptake due to the arrest of cells in G1 phase.

show abstract

Identification of carbonylated proteins from monocytic cells under diabetes‐induced stress conditions

Nair

Nedungadi

Mishra

et al. 2021

Biomedical Chromatography

View full text Add to dashboard Cite

Diabetes is a metabolic disorder characterized by the presence of elevated glucose in the blood and enhanced oxidative stress. It affects the cellular homeostasis that leads to the development of micro‐and macro‐vascular complications. Monocytes are the primary immune cells present in the circulatory system. Under high‐glucose conditions, the cells undergo oxidative stress and secrete reactive oxygen species. The enhanced release of reactive species is known to modify biomolecules like proteins and nucleic acids. Protein carbonylation, one of the most harmful and irreversible protein modifications, is considered as a key player in the progression of diabetes and associated complications. Hence, the present study explores the identification of carbonylated proteins from the monocytes under diabetic stress and determination of their site of modification. Combined avidin affinity chromatography and bottom‐up proteomics experiments identified 13 consistently expressed carbonylated proteins. Most of the identified proteins were reported to have altered functions under diabetic conditions that contribute to the development of diabetes‐associated inflammation and complications. We were able to determine oxidative stress‐induced modifications on Lys, Val, Ile, Cys, Thr and Asp residues.

show abstract

Adenylyl Cyclase-Associated Protein 1 Is a Receptor for Human Resistin and Mediates Inflammatory Actions of Human Monocytes

Cited by 220 publications

References 45 publications

Resistin increases the expression of NOD2 in mouse monocytes

Resistin increases the expression of NOD2 in mouse monocytes

Resistin causes G1 arrest in colon cancer cells through upregulation of SOCS3

Identification of carbonylated proteins from monocytic cells under diabetes‐induced stress conditions

Contact Info

Product

Resources

About